РЕЗЮМЕ. У цьому рукописі представлено ідентифікацію та прогнозну структурну характеристику нового GLUT1-ортолога людини, транспортера мелатоніну (MelT), в рослинах (особливо в рисі) та його функціональну здатність транспортувати мелатонін. Показано, що мелатонін зв’язується з тими самими залишками в GLUT1, що й глюкоза. Моделювання гомології та докінганаліз передбачили загальну гомологію послідовності OsMelT з GLUT1. Було передбачено, що білок містить 12 трансмембранних спіралей та цукротранспортний домен PF00083.24 (відповідальний за зв’язування та вивільнення мелатоніну). Скінець був більш структурованим порівняно з Nкінцем, і по всьому білку були виявлені сайти фосфорилювання. Висхідний аналіз MelT показав наявність цисактивних мотивів, пов’язаних з абсцизовою кислотою, регуляцією мелатоніну та індукцією як до абіотичного, так і до біотичного стресу. Дослідження експресії підтвердили посилення регуляції OsMelT у присутності глюкози та підвищених концентрацій екзогенного мелатоніну. В цілому, дослідження передбачило функціональну здатність OsMelT транспортувати мелатонін і підтримувати поглинання та мобілізацію біомолекули за вищих концентрацій.
Ключові слова: мелатонін, транспортер, філограма, моделювання гомології, докінганаліз, експресія генів, біоінформатика
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