Два види роду Galinsoga, G. parviflora Cav. та G. quadriradiata Ruiz & Pav. належать до найбільш успішних інвазійних рослин, які завдають суттєвої шкоди природним та агроекосистемам. Їх природний ареал простягається від Північної до Південної Америки, а адвентивна частина ареалу охоплює всі материки, крім Антарктиди. Не дивлячись на практичне значення G. parviflora та G. quadriradiata, генетичне різноманіття європейських популяцій цих видів все ще залишається недослідженим. В цій роботі ми використали ISSR маркери для дослідження українських популяцій G. parviflora та G. quadriradiata та порівняли їх із рослинами з території Польщі, Литви та Португалії. Отримані результати засвідчують низьке генетичне різноманіття (індекс Шенона I = 0,124) популяцій G. quadriradiata, вірогідною причиною чого може бути малий розмір вихідної популяції, завезеної з Америки до Старого Світу. Водночас, рівень генетичного різноманіття у популяціях G. parviflora є суттєво вищим (I = 0,254). Деякі генотипи G. parviflora мають широке географічне розповсюдження та, водночас, різні генотипи зустрічаються на спільній території. Отримані дані добре узгоджується із гіпотезою про втечу з ботанічних садів як основне джерело походження інвазійних видів роду Galinsoga. Серед досліджених зразків виявлено декілька форм гібридної природи, які імовірно походять від гібриду між G. parviflora та G. quadriradiata внаслідок наступних зворотних схрещувань з одним із батьківських видів.
Ключові слова: баркодинг, біологічне різноманіття, генетичний поліморфізм, молекулярна геноміка, міжвидова гібридизація, молекулярні маркери
Повний текст та додаткові матеріали
У вільному доступі: PDFЦитована література
AL-Kiyyam, M.A., Shibli, R.A., Zatimeh, A., Tahtamouni, R.W., AlQudah, T.S., and Hassan, S., Evaluation of genetic diversity in jordanian Solanum nigrum plants and genetic stability of in vitro grown plant using (AFLP) technique, Jordan J. Biol. Sci., 2024, vol. 17, no. 1. https://doi.org/10.54319/jjbs/170111
Baldwin, B.G., Wessa, B.L., and Panero, J.L., Nuclear rDNA evidence for major lineages of helenioid Heliantheae (Compositae), Syst. Bot., 2002, vol. 27, no. 1, pp. 161–198. https://doi.org/10.1043/0363-6445-27.1.161
Bublyk, O., Andreev, I., Kalendar, R., Spiridonova, K., and Kunakh, V., Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity, Biologia, 2013, vol. 68, no. 4, pp. 613–620. https://doi.org/10.2478/s11756-013-0192-4
Canne, J.M., The Systematics of the Genus Galinsoga (Compositae: Heliantheae), Ohio State Univ., 1976.
Canne, J.M., A revision of the genus Galinsoga (Compositae: Heliantheae), Rhodora, 1977, vol. 79, no. 819, pp. 319–389.
Canne, J.M., Cytological and morphological observations in Galinsoga and related genera (Asteraceae), Rhodora, 1983, vol. 85, no. 843, pp. 355–366.
China Plant BOL Group; Li, D.Z., Gao, L.M., Li, H.T., Wang, H., Ge, X.J., Liu, J.Q., Chen, Z.D., Zhou, S.L., Chen, S.L., Yang, J.B., Fu, C.X., Zeng, C.X., Yan, H.F., Zhu, Y.J., Sun, Y.S., Chen, S.Y., Zhao, L., Wang, K., Yang, T., and Duan, G.W., Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants, Proc. Natl. Acad. Sci. U. S. A., 2011, vol. 108, no. 49, pp. 19641–19646. https://doi.org/10.1073/pnas.1104551108
Damalas, C.A., Distribution, biology, and agricultural importance of Galinsoga parviflora (Asteraceae), Weed B-iol. Manage., 2008, vol. 8, no. 3, pp. 147–153. https://doi.org/10.1111/j.1445-6664.2008.00290.x
de Tomás, C. and Vicient, C.M., The genomic shock hypothesis: genetic and epigenetic alterations of transposable elements after interspecific hybridization in plants, Epigenomes, 2023, vol. 8, no. 1, p. 2. https://doi.org/10.3390/epigenomes8010002
Domblides, A. and Domblides, E., Rapid genetic assessment of carrot varieties based on AFLP analysis, J. Hortic., 2023, vol. 9, no. 3, p. 298. https://doi.org/10.3390/horticulturae9030298
Duistermaat, H., Gottschlich, G., Kadereit, J., Kilian, N., Rottensteiner, W.K., Uhlemann, I., Vitek, E., Vogt, R., and Wagenitz, G., Asteraceae Dumort. – Körbchenblütler, in Exkursionsflora für Istrien, Klagenfurt: Naturwissenschaftlicher Verein für Kärnten, 2014, pp. 770–774.
Earl, D.A. and von Holdt, B.M., STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method, Conserv. Genet. Resour., 2012, vol. 4, pp. 359–361. https://doi.org/10.1007/s12686-011-9548-7
Galera, H. and Sudnik-Wójcikowska, B., Central European botanic gardens as centres of dispersal of alien plants, Acta Soc. Bot. Pol., 2010, vol. 79, no. 2, pp. 147–156.
Galinsoga Ruiz and Pav. in GBIF, Secretariat GBIF Backbone Taxonomy, Checklist, 2023. https://doi.org/10.15468/39omei
Ghaffarian, S. and Mohammadi, S.A., Genetic diversity and population structure of Melissa officinalis L. from Iran as reveled by retrotransposon insertional polymorphism, Genet. Resour. Crop Evol., 2023, vol. 70, no. 8, pp. 2521–2532. https://doi.org/10.1007/s10722-023-01580-1
Gill, B.A., Musili, P.M., Kurukura, S., Hassan, A.A., Goheen, J.R., Kress, W.J., Kuzmina, M., Pringle, R.M., and Kartzinel, T.R., Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna, Mol. Ecol. Resour., 2019, vol. 19, no. 4, pp. 838–846. https://doi.org/10.1111/1755-0998.13001
Gopinathan, M.C. and Babu, C.R., Cytogenetics of Galinsoga parviflora Cav. and G. ciliata (Raf.) Blake, and their natural hybrids (Asteraceae), New Phytol., 1982, vol. 91, no. 3, pp. 531–539. https://doi.org/10.1111/j.1469-8137.1982.tb03331.x
Gui, F.R., Wan, F.H., and Guo, J.Y., Determination of the population genetic structure of the invasive weed Ageratina adenophora using ISSR-PCR markers, Russ. J. Plant Physiol., 2009, vol. 56, no. 3, pp. 410–416. https://doi.org/10.1134/S1021443709030157
Haskell, G. and Marks, G.E., Chromosome ecology of British Galinsoga species, New Phytol., 1952, vol. 51, no. 3, pp. 382–387.
Heukels, H. and van der Meijden, R., Heukels’ Flora van Nederland 23e Druk, Wolters-Noordhoff, 2005.
Huangfu, C.H., Song, X.L., and Qiang, S., ISSR variation within and among wild Brassica juncea populations: implication for herbicide resistance evolution, Genet. Resour. Crop Evol., 2009, vol. 56, no. 7, pp. 913–924. https://doi.org/10.1007/s10722-009-9410-x
Ivanovych, Y. and Volkov, R., Genetic relatedness of sweet cherry (Prunus avium L.) cultivars from Ukraine determined by microsatellite markers, J. Hortic. Sci. Biotechnol., 2018, vol. 93, no. 1, pp. 64–72. https://doi.org/10.1080/14620316.2017.1342568
Ivanovych, Y.I., Udovychenko, K.M., Bublyk, M.O., and Volkov, R.A., ISSR-PCR fingerprinting of Ukrainian sweet cherry (Prunus avium L.) cultivars, Cytol. Genet., 2017, vol. 51, no. 1, pp. 40–47. https://doi.org/10.3103/s0095452717010066
Jursík, M., Soukup, J., Venclová, V., and Holec, J., Seed dormancy and germination of Shaggy soldier (Galinsoga ciliata Blake.) and Common lambsquarter (Chenopodium album L.), Plant Soil Environ., 2003, vol. 49, no. 11, pp. 511–518. https://doi.org/10.17221/4186-PSE
Kolter, A. and Gemeinholzer, B., Internal transcribed spacer primer evaluation for vascular plant metabarcoding, Metabarcoding Metagenomics, 2021, vol. 5, p. e68155. https://doi.org/10.3897/mbmg.5.68155
Kress, W.J., Wurdack, K.J., Zimmer, E.A., Weigt, L.A., and Janzen, D.H., Use of DNA barcodes to identify flowering plants, Proc. Natl. Acad. Sci. U. S. A., 2005, vol. 102, no. 23, pp. 8369–8374. https://doi.org/10.1073/pnas.0503123102
Kucewicz, M. and Gojło, E., Influence of achene heteromorphism on life-cycle traits in the annual weed gallant soldier (Galinsoga parviflora Cav.), Flora, 2014, vol. 209, no. 11, pp. 649–654. https://doi.org/10.1016/j.flora.2014.08.010
Landjeva, S., Ganeva, G., Korzun, V., Palejev, D., Chebotar, S., and Kudrjavtsev, A., Genetic diversity of old bread wheat germplasm from the Black Sea region evaluated by microsatellites and agronomic traits, Plant Genet. Resour., 2015, vol. 13, no. 2, pp. 119–130. https://doi.org/10.1017/S1479262114000781
Li, X.C., Qi, S.Y., Yao, J., and Yang, L., Genetic diversity and differentiation of invasive plant Galinsoga quadriradiata populations in China, Chin. J. Ecol., 2015, vol. 34, no. 12, pp. 3306–3312.
Liu, R.L., Yang, Y.B., Lee, B.R., Liu, G., Zhang, W.G., Chen, X.Y., Song, X.J., Kang, J.Q., and Zhu, Z.H., The dispersal-related traits of an invasive plant Galinsoga quadriradiata correlate with elevation during range expansion into mountain ranges, AoB Plants, 2021, vol. 13, no. 3, p. plab008. https://doi.org/10.1093/aobpla/plab008
Mykhaletskyi, Y., structureHarvesterWebRunner, GitHub, 2024. https://github.com/yuremboo/structureHarvesterWebRunner.
Nei, M. and Li, W., Mathematical model for studying genetic variation in terms of restriction endonucleases, Proc. Natl. Acad. Sci. U. S. A., 1979, vol. 76, no. 10, pp. 5269–5273. https://doi.org/10.1073/pnas.76.10.5269
Peakall, R. and Smouse, P.E., GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update, Bioinformatics, 2012, vol. 28, no. 19, pp. 2537–2539. https://doi.org/10.1093/bioinformatics/bts460
Pelser, P.B., Kennedy, A.H., Tepe, E.J., Shidler, J.B., Nordenstam, B., Kadereit, J.W., and Watson, L.E., Patterns and causes of incongruence between plastid and nuclear Senecioneae (Asteraceae) phylogenies, Am. J. Bot., 2010, vol. 97, no. 5, pp. 856–873. https://doi.org/10.3732/ajb.0900287
Perrier, X., Flori, A., and Bonnot, F., Data analysis methods, In Hamon, P., Seguin, M., Perrier, X., and Glaszmann, J.C., Eds., Genetic Diversity of Cultivated Tropical Plants, Enfield: Science Publ., 2003, pp. 43–76.
Pietrusiewicz, J., Domaciuk, M., and Bednara, J., Different pathways of embryo sac development in Galinsoga parviflora Cav., Acta Biol. Cracov., 2005, vol. 47, no. 1.
Porebski, S., Bailey, L.G., and Baum, B.R., Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components, Plant Mol. Biol. Rep., 1997, vol. 15, no. 1, pp. 8–15. https://doi.org/10.1007/BF02772108
POWO Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew, 2024. http://www.plantsoftheworldonline.org/.
Pritchard, J.K., Stephens, M., and Donnelly, P., Inference of population structure using multilocus genotype data, Genetics, 2000, vol. 155, no. 2, pp. 945–959. https://doi.org/10.1093/genetics/155.2.945
Pysek, P., Prach, K., and Mandák, B., Invasions of alien plants into habitats of Central European landscape: an historical pattern, in Plant Invasions: Ecological Mechanisms and Human Responses, 1998, pp. 23–32.
Rabokon, A.M., Blume, R.Y., Sakharova, V.G., Chopei, M.I., Afanasieva, K.S., Yemets, A.I., Rakhmetov, B., Pirko, Y.V., and Blume, Y.B., Genotyping of interspecific Brassica rapa hybrids implying β-tubulin gene intron length polymorphism (TBP/cTBP) assessment, Cytol. Genet., 2023, vol. 57, no. 6, pp. 538–549.
Rieseberg, L.H., Chromosomal rearrangements and speciation, Trends Ecol. Evol., 2001, vol. 16, no. 7, pp. 351–358. https://doi.org/10.1016/s0169-5347(01)02187-5
Rivera, P., Villaseñor, J.L., Terrazas, T., and Panero, J.L., The importance of the Mexican taxa of Asteraceae in the family phylogeny, J. Syst. Evol., 2021, vol. 59, no. 5, pp. 935–952. https://doi.org/10.1111/jse.12681
Smith, A.L., Hodkinson, T.R., Villellas, J., et al., Global gene flow releases invasive plants from environmental constraints on genetic diversity, Proc. Natl. Acad. Sci. U. S. A., 2020, vol. 117, no. 8, pp. 4218–4227. https://doi.org/10.1073/pnas.1915848117
Smýkal, P., Bačová-Kerteszová, N., Kalendar, R., Corander, J., Schulman, A.H., and Pavelek, M., Genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm assessed by retrotransposon-based markers, Theor. Appl. Genet., 2011, vol. 122, no. 7, pp. 1385–1397. https://doi.org/10.1007/s00122-011-1539-2
Tommasi, N., Biella, P., Guzzetti, L., Lasway, J.V., Njovu, H.K., Tapparo, A., Agostinetto, G., Peters, M.K., Steffan-Dewenter, I., Labra, M., and Galimberti, A., Impact of land use intensification and local features on plants and pollinators in Sub-Saharan smallholder farms, Agric. Ecosyst. Environ., 2021, vol. 319, no. 9, p. 107560. https://doi.org/10.1016/j.agee.2021.107560
Tunaitienė, V., Naugžemys, D., Patamsytė, J., and Žvingila, D., Gradient of genetic diversity of Erigeron annuus in the part of invasive European range, Bota Lith., 2015, vol. 21, no. 2, pp. 81–88. https://doi.org/10.1515/botlit-2015-0011
Twardovska, M.O., Drobyk, N.M., Mel’nyk, V.M., Konvalyuk, I.I., and Kunakh, V.A., Genome variability of some Gentiana L. species in nature and in culture in vitro: RAPD-analysis, Biopolym. Cell, 2010, vol. 26, no. 6, pp. 499–507. https://doi.org/10.7124/bc.00017A
Tynkevich, Y.O., Shelyfist, A.Y., Kozub, L.V., Hemleben, V., Panchuk, I.I., and Volkov, R.A., 5S ribosomal DNA of genus Solanum: molecular organization, evolution, and taxonomy, Front. Plant Sci., 2022, vol. 13, p. 852406. https://doi.org/10.3389/fpls.2022.852406
Tynkevich, Y.O., Yakobyshen, D.V., Cherkazianova, A.S., Shelyfist, A.Y., and Volkov, R.A., Intragenomic polymorphism of the ITS1-5.8S-ITS2 region in invasive species of the genus Reynoutria, Cytol. Genet., 2024, vol. 58, no. 6.
Varshney, R.K., Chabane, K., Hendre, P.S., Aggarwal, R.K., and Graner, A., Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys, Plant Sci., 2007, vol. 173, no. 6, pp. 638–649. https://doi.org/10.1016/j.plantsci.2007.08.010
Volkov, R.A., Borisjuk, N.V., Panchuk, I.I., Schweizer, D., and Hemleben, V., Elimination and rearrangement of parental rDNA in the allotetraploid Nicotiana tabacum, Mol. Biol. Evol., 1999, vol. 16, no. 3, pp. 311–320. https://doi.org/10.1093/oxfordjournals.molbev.a026112
Volkov, R.A., Kozeretska, I.A., Kyryachenko, S.S., Andreev, I.O., Maidanyuk, D.N., Parnikoza, I.Y., and Kunakh, V.A., Molecular evolution and variability of ITS1–ITS2 in populations of Deschampsia antarctica from two regions of the maritime Antarctic, Polar Sci., 2010, vol. 4, no. 3, pp. 469–478. https://doi.org/10.1016/j.polar.2010.04.011
Wang, Z., Wang, J.E., Wang, X.M., Gao, H.W., Dzyubenko, N.I., and Chapurin, V.F., Assessment of genetic diversity in Galega officinalis L. using ISSR and SRAP markers, Genet. Resour. Crop Evol., 2012, vol. 59, pp. 865–873. https://doi.org/10.1007/s10722-011-9727-0
Ward, S.M., Reid, S.D., Harrington, J., Sutton, J., and Beck, K.G., Genetic variation in invasive populations of yellow toadflax (Linaria vulgaris) in the western United States, Weed Sci., 2008, vol. 56, no. 3, pp. 394–399. https://doi.org/10.1614/WS-07-157.1
WFO World Flora Online, 2023. Available from: http://www.worldfloraonline.org/. Accessed August 14, 2024.
Xu, S.Z., Li, Z.Y., and Jin, X.H., DNA barcoding of invasive plants in China: A resource for identifying invasive plants, Mol. Ecol. Resour., 2018, vol. 18, no. 1, pp. 128–136. https://doi.org/10.1111/1755-0998.12715
Yang, J., Tang, L., Guan, Y.L., and Sun, W.B., Genetic diversity of an alien invasive plant Mexican sunflower (Tithonia diversifolia) in China, Weed Sci., 2012, vol. 60, no. 4, pp. 552–557. https://doi.org/10.2307/23363042
Yavorska, O., New records of some alien plants in the Kyiv urban area, Visn. Lviv Univ. Biol. Ser., 2008, vol. 48, pp. 44–48.
Ye, W.H., Li, J., Cao, H.L., and Ge, X.J., Genetic uniformity of Alternanthera philoxeroides in South China, Weed Res., 2003, vol. 43, no. 4, pp. 297–302. https://doi.org/10.1046/j.1365-3180.2003.00346.x
Ye, W.H., Mu, H.P., Cao, H.L., and Ge, X.J., Genetic structure of the invasive Chromolaena odorata in China, Weed Res., 2004, vol. 44, no. 2, pp. 129–135. https://doi.org/10.1111/j.1365-3180.2004.00381.x
Zhai, Q., Wang, W.B., Qu, B., and Shao, M.N., A supplementary study of two species of Galinsoga in the Liaoning Province, Bull. Bot. Res., 2018, vol. 38, no. 3, pp. 338–342.
Zhang, Q., Ye, J.F., Le, C.T., Njenga, D.M., Rabarijaona, N.R., Omollo, W.O., Lu, L.M., Liu, B., and Chen, Z.D., New insights into the formation of biodiversity hotspots of the Kenyan flora, Diversity Distrib., 2022, vol. 28, no. 12, pp. 2696–2711. https://doi.org/10.1111/ddi.13624
Zhang, W.G., Song, X.J., Petri, L., Liu, G., Chen, X.Y., Liu, R.L., Huang, F.F., Zou, J.B., and Zhu, Z.H., Elevated nitrogen and co-evolution history with competitors shape the invasion process of Galinsoga quadriradiata, J. Plant Ecol., 2024, vol. 17, no. 4, p. rtae047. https://doi.org/10.1093/jpe/rtae047