Біосинтезовані металеві наночастинки мають унікальні властивості та активно вивчаються як по-тенційні антимікробні агенти. В даній роботі описано «зелений» синтез наночастинок срібла, здійснений із використанням водного екстракту з листя Chaenomeles japonica як біологічної матриці, а також отримання їх кон’югатів з антибіотиком цефтриаксоном. УФ-видимі спектри поглинання підтвердили формування біосинтезованих наночастинок срібла (Ch-AgNPs) та їх кон’югатів із цефтриаксоном (Ch-AgNPs-Cfx), що характеризують піками поглинання поверхневого плазмонного резонансу при 475 і 478 нм, відповідно. Аналіз інфрачервоних спектрів із перетворенням Фур’є виявив участь гідроксильних і карбоксильних функціональних груп фенольних і ароматичних сполук, флавоноїдів, терпеноїдів, спиртів і карбонових кислот рослинного екстракту в процесі біовідновлення Ag+ до Ag0, а також участь карбонільних та амінних груп білків у стабілізації наночастинок срібла. Антимікробну активність Ch-AgNPs, Ch-AgNPs-Cfx та цефтриаксону оцінювали, використовуючи штам Pseudomonas aeruginosa, стійкий до деяких цефалоспоринів. Виявлено дозозалежне інгібування P. aeruginosa за дії Ch-AgNPs та Ch-AgNPs-Cfx у діапазоні 2,5–20 мкг/диск, яке перевищувало активність цефтриаксону у тих самих дозах, характеризуючи обидва типи біосинтезованих наночастинок як перспективні бактерицидні агенти. Подальші дослідження Ch-AgNP та Ch-AgNPs-Cfx можуть бути спрямовані на створення ефективних засобів для зниження доз антибіотиків без втрати антибактеріальної активності проти стійких патогенів.
Ключові слова: наночастинки срібла, «зелений» синтез, Chaenomeles japonica, кон’югати з антибіотиками, цефтриаксон, поверхневий плазмонний резонанс, ІЧ-спектроскопія, Pseudomonas aeruginosa, антимікробна активність
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