РЕЗЮМЕ. Зростає усвідомлення того, що ефективна та регулярна динаміка мітохондрій покращує функцію серця і впливає на якість життя. Мелатонін – це основний гормон епіфізу, який оптимізує динаміку мітохондрій при багатьох порушеннях серцевої діяльності. З огляду на цей факт, ми вводили здоровим щурам мелатонін упродовж усієї доби, щоб дослідити зміни у динаміці мітохондрій лівого шлуночка серця і наприкінці ночі, і протягом дня. Двадцять самців лінії Вістар (віком 3–4 міс) були рандомізовано розподілені у групу контролю (C; n = 10) та групу мелатоніну (MEL; додавання 10 мг/кг мелатоніну до питної води, n = 10). На п’ятий день дослідження рандомізовано обрали по 5 щурів з груп та піддали їх евтаназії о 08:00, а інших тварин, по 5 щурів з обох груп, умертвили о 20:00 і взяли зразки тканини з лівого шлуночка (LV). Результати кількісної ПЦР у реальному часі і Вестерн-блот аналізу продемонстрували, що мелатонін відіграє превентивну роль у злитті мітохондрій та мітофагії по осі DRP1/FIS1 та BNIP3/NIX, відповідно. Крім того, введення мелатоніну значно знизило активацію P21, індукувало зупинку клітинного циклу, P27, зрештою, регулювало сигнали каспазо-залежного мітохондріального апоптозу залежно від часу. Наші результати показують, що мелатонін може бути терапевтичним засобом захисту біоенергетичної динаміки мітохондрій у серці.
Ключові слова: мелатонін, серце, апоптоз, динаміка мітохондрій, мітофагія
Повний текст та додаткові матеріали
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