Discrimination of interspecific hybrids in natural populations of Amur sturgeon fishes using multilocus RAPD-PCR markers

Chelomina G.N., Rozhkovan K.V., Ivanov S.A.

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RAPD-PCR analysis of 46 individuals of sturgeons from Amur River has been carried out. Genetic status of Amur sturgeon Acipenser schrenckii Brandt, 1869 and kaluga Huso dauricus Georgi, 1775 native populations has been estimated. Genetic evidences of hybrid origin for two phenotypical hybrids were obtained; estimations of genetic distances between species and hybrids appeared to be at interspecific level. The exact test for differentiation of populations (Exact test) and multidimensional scaling (MDS) analysis were estimated to be the most effective for species and hybrid discrimination, respectively. According to data obtained populations of sturgeon fishes which inhabit Amur River maintained an essential level of genetic variability; the presence of hybrids is regarded as one of risk factors. Multilocus RAPD-PCR markers admit as the convenient and reliable tool for genetic monitoring of Amur River sturgeons to preserve their gene pool.

Tsitologiya i Genetika 2008, vol. 42, no. 5, pp. 61-71

E-mail: chelomina ibss.dvo.ru, amursturgeon mail

Chelomina G.N., Rozhkovan K.V., Ivanov S.A. Discrimination of interspecific hybrids in natural populations of Amur sturgeon fishes using multilocus RAPD-PCR markers, Tsitol Genet., 2008, vol. 42, no. 5, pp. 61-71.