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Biolictic transformation of soybean by new selective marker gene conferring resistance to dinitroanilines
[Free Full Text (pdf)] ![]() Biolistic transformation of soybean by construction carrying a mutant α-tubulin gene as selective marker gene conferring resistance to dinitroaniline herbicides, and by additional construction pAHTUB1 carrying a full-length barley β-tubulin gene for correct co-expression of exogeneous tubulin in cells of transgenic soybean lines has been realized. It was established that 10 ΅M trifluralin is the most optimal selective concentration to pick up transformed soybean lines. Transgenic nature of offained regenerants was confirmed by Southern blotting hybridization using specific probe to selective α-tubulin gene.
Tsitologiya i Genetika 2008, vol. 42, no. 6, pp. 61-68
E-mail: alyemets
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