РЕЗЮМЕ. Хіміотерапевтичні препарати широко відомі своїми несприятливими побічними ефектами під час лікування онкологічних захворювань. Одним із додаткових підходів до вирішення цієї дилеми може бути використання природних сполук, які можуть оптимально протидіяти пошкодженню клітин під час хіміотерапії. Метою цього дослідження було оцінити природний флавоноїд, хризин (5,7-дигідроксифлавон), на предмет його терапевтичних імуномодулюючих властивостей у поєднанні з хіміотерапевтичним препаратом циклофосфамідом (ЦФ). У цьому дослідженні використовували самців-альбіносів мишей лінії Вістар. Було проведено дослідження гострої токсичності, гемолізу, фагоцитозу, цитотоксичності природних кілерів (ПК) та оксидативного стресу. Для оцінки експресії маркерів запалення було використано ПЛР-ЗТ, ІФА та Вестерн-блоттинг. Такі результати дослідження, як індекс фагоцитозу (0,009 ± 0,001), рівень цитотоксичності природних кілерів (61,10 ± 4,99 %), показники експресії перфорину (0,45 ± 0,05 разів) та гранзиму (0,86 ± 0,01 разів), антиоксидантних ферментів печінки, GSH (27,75 ± 1,54 мкг/мг), СОД (7,10 ± 0,35 од/мг) та каталази (249,06 ± 31,30 мкМ/хв/мг), а також антиоксидантних ферментів селезінки і печінки, GSH (20,88 ± 0,74 мкг/мг), СОД (7,10 ± 0,35 од/мг) і каталази (249,06 ± 31,30 мкМ/хв/мг) у групах, яким вводили ЦФ, порівняли з показниками груп, яким вводили ЦФ+хризин. Було виявлено значні підвищення значень: 0,016 ± 0,001, 82,73 ± 2,87 %, 0,77 ± 0,08 разів, 1,11 ± 0,02 разів, 47,60 ± 3,02 мкг/мг, 08,97 ± 0,42 од/мг, 467,19 ± 15,92 мкМ/хв/мг, 29,02 ± 1,59 мкг/мг, 5,17 ± 0,94 од./мг, 310,29 ± 9,1330 мкМ/хв/мг, відповідно. Гістопатологічне дослідження показало, що групи, які отримували ЦФ+хризин, могли відновитися після пошкодження клітин, викликаного лікуванням за допомогою ЦФ. Результати показали цитопротекторну роль хризину, який можна вводити в якості надійної додаткової терапії у поєднанні з ЦФ під час хіміотерапії.
Ключові слова: хіміотерапевтичні препарати, циклофосфамід, хризин, запалення, токсичність
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