РЕЗЮМЕ. Конексини (Cx) відіграють у клітинах роль, яка і пов’язана з щілинними контактами, і незалежна від них, і їхня локалізація має важливе значення для їхньої функції в клітинних процесах. Окрім мембранної локалізації, конексини також можуть бути локалізовані в цитоплазмі та ядрі, особливо в ракових клітинах. На різних стадіях раку спостерігається диференційована локалізація конексинів, включно з Cx32. Cx32 був підвищений і спостерігався в цитоплазмі клітин у лімфатичних вузлах метастазів зразків раку молочної залози порівняно з первинними пухлинами. Однак, значення підвищення експресії Cx32 та зміни клітинної локалізації Cx32 при епітеліальномезенхімальному переході (ЕМП) невідоме. Щоб визначити, чи протягом одного тижня надмірна експресія та/або локалізація Cx32 індукує процес ЕМП, ми спочатку дослідили клітинну локалізацію Cx32 у клітинах MCF10A та MDAMB231 у різні часові точки, використовуючи Вестернблот та RTПЛР, а також імунофарбування за допомогою конфокальної мікроскопії. Потім ми співвіднесли зміни експресії та локалізації Cx32 з експресією маркерів ЕМП. Ми показали, що Cx32 має змінену клітинну локалізацію, а гіперекспресія Cx32 підвищує рівень Slug, тоді як експресію Eкадгерину та Snail в MDAMB231 знижує впродовж 7 днів. На противагу цьому, в клітинах MCF10ACx32 експресія Eкадгерину та віментину знижувалась порівняно з контролем впродовж 7 днів, а експресія ядерного Cx32 та Zeb2 в клітинах MCF10A була подібною до контролю. Отримані результати свідчать про раніше невідомий зв’язок між Cx32 та регуляцією процесу ЕМП, який залежить від часу.
Ключові слова: рак молочної залози, конексин32, епітеліальномезенхімальний перехід, ядро, локалізація
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