РЕЗЮМЕ. Кальпаїни – це залежні від кальцію внутрішньоклітинні нейтральні цистеїнові протеази, відомі своєю важливою роллю, яку вони відіграють у post mortem протеолізі та тендеризації м’яса риби. У цьому дослідженні було проведено вивчення характеристик родини кальпаїнів (CAPN) у пангасіанодона великоокого (Pangasianodon hypophthalmus), який є одним із основних видів, використовуваних у рибоводному господарстві В’єтнаму. Спочатку було проведено пошук родини кальпаїнів у базі даних геному сомів, потім аналіз класифікації, філогенетичних відносин і структури гену. Було виявлено, що в геномі пангасіанодона великоокого 14 кальпаїнів є ортологами для інших видів хребетних, і проведено їхню класифікацію з поділом на типові та атипові кальпаїни згідно з їхньою структурою. Далі було проведено вивчення експресії транскрипційних генів, типових генів CAPN1, 2, 3, 11, 13, у тканинах м’язів, печінки і мозку пангасіанодона великоокого. Результати продемонстрували, що експресія генів CAPN2 і CAPN13 була ледь помітною, у той час як експресія генів CAPN1, 3, 11 була значною і спостерігалась у всіх трьох типах тканин. У тканині м’язів пангасіанодона великоокого наявність транскрипту CAPN3 майже втричі та у 27 разів перевищувала показники CAPN11 і CAPN1, відповідно. Наші результати дозволяють припустити, що CAPN3 у P. hypophthalmus також є кальпаїном, характерним для м’язів, як було відмічено у повідомленнях щодо інших видів. Результати цього дослідження надають дані для подальшого вивчення кальпаїнів та їхнього генетичного різноманіття, яке може корелювати з текстурою м’язів пангасіанодона великоокого.
Ключові слова: родина кальпаїнів, CAPN, профіль експресії, Pangasianodon hypophthalmus, пангасіанодон великооки

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