Calpains are calciumdependent intracellular neutral cysteine proteases that have been known to play an important role in postmortem proteolysis and meat tenderisation in fish. In this study, the calpain family (CAPN) in striped catfish (Pangasianodon hypophthalmus) which is one of major aquaculture species in Vietnam was characterized. Firstly, the catfish genome database was searched for calpain gene family, then, classification, phylogenetic relationship and gene structure were analyzed. In striped catfish genome, 14 calpain genes were found that are orthologs to other vertebrate species, which were classified into typical and atypical calpains according to their structures. Next, the transcriptional gene expression of typical CAPN1, 2, 3, 11, 13 genes in muscle, liver and brain tissues of the striped catfish were examined. The results showed that gene expression of CAPN2 and CAPN13 genes was barely detectable, while that of CAPN1, 3, 11 genes was widely detected in all three types of tissues. In striped catfish muscle tissue, CAPN3 transcript abundance was nearly three and 27 fold greater than CAPN11 and CAPN1, respectively. Our results suggest that CAPN3 in P. hypophthalmus is also a musclespecific calpain, which had been reported in other species. The results of this study provide a data resource for further research on the function of calpain genes and their genetic diversity that might be correlated with muscle texture in striped catfish.
Keywords: Calpain family, CAPN, expression profile, Pangasianodon hypophthalmus, striped catfish
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