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Характеристика высокомолекулярных субъединиц глютенина дикой полбы (Triticum dicoccoides)

Орловская О.А., Яцевич К.К., Вакула С.И., Хотылева Л.В., Кильчевский А.В.

Оригінальна работа 




Проведена идентификация и молекулярный анализ высокомолекулярных субъединиц глютенина (HMW-GS) образца дикой полбы T. dicoccoides К5199. Выявлены новые HMW субъединицы глютенина 1АхTd и 1ByTd. Определены нуклеотидные последовательности генов 1АхTdи 1ByTd(коды доступа в базе данных GenBank MH475136 и MG897125 соответственно). Установлено, что кодирующая последовательность гена 1АхTdимеет наибольшее сходство с геном 1Ах1 T. aestivum(99,7 %), в то время как последовательность гена 1ByTd– сгенами других образцов T. dicoccoides(98,5 и 97,6 %). Первичная и вторичная структуры белка исследованных HMW-GSпозволяют прогнозировать высокий вклад в хлебопекарные cвойства субъединицы 1АхTd и средний вклад ‑ субъединицы 1ByTd. Оценка важнейших критериев качества зерна показала повышенное содержание белка и клейковины, но сниженные реологические характеристики клейковины T. dicoccoidesК5199.

РЕЗЮМЕ. Проведено ідентифікацію і молекулярний аналіз високомолекулярних субодиниць глютенина (HMW-GS) зразка дикої полби T. dicoccoides К5199. Виявлено нові HMW субодиниці глютенина 1АхTd і 1ByTd. Визначено нуклеотидні послідовності генів 1АхTd і 1ByTd (коди доступу в базі даних GenBank MH475136 і MG897125 відповідно). Встановлено, що кодує послідовність гена 1АхTd має найбільшу схожість з геном 1Ах1T. aestivum (99,7 %), в той час як послідовність гена 1ByTd‑ з генами інших зразків T. dicoccoides (98,5 і 97,6 %). Первинна і вторинна структури білка досліджених HMW-GS дозволяють прогнозувати високий внесок в хлібопекарські cвойства субодиниці 1АхTd і середній внесок субодиниці 1ByTd. Оцінка найважливіших критеріїв якості зерна показала підвищений вміст білка і клейковини, але знижені реологічні характеристики клейковини T. dicoccoides К5199.

Ключові слова: T. dicoccoides, HMW-GS, SDS-електрофорез, секвенування, первинна та вторинна структура білка, якість зерна
T. dicoccoides, HMW-GS,SDS-электрофорез, секвенирование, первичная и вторичная структура белка, качество зерна

Цитологія і генетика 2020, том 54, № 3, C. 28-36

  • Институт генетики и цитологии Национальной академии наук Беларуси, Минск

E-mail: O.Orlovskaya igc.by

Орловская О.А., Яцевич К.К., Вакула С.И., Хотылева Л.В., Кильчевский А.В. Характеристика высокомолекулярных субъединиц глютенина дикой полбы (Triticum dicoccoides), Цитологія і генетика., 2020, том 54, № 3, C. 28-36.

В "Cytology and Genetics". Якщо тільки можливо, цитуйте статтю по нашій англомовній версії:
O. A. Orlovskaya, K. K. Yatsevich, S. I. Vakula, L. V. Khotyleva & A. V. Kilchevsky Characterization of High Molecular Weight Glutenin Subunits in Wild Emmer Wheat (Triticum dicoccoides), Cytol Genet., 2020, vol. 54, no. 3, pp. 199–205
DOI: 10.3103/S009545272003010X


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