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Characterization of high molecular weight glutenin subunits in wild emmer wheat (Triticum dicoccoides)
SUMMARY. The identification and molecular analysis of high molecular weight glutenin subunits (HMW-GS) of the wild emmer wheat T. dicoccoides K5199 were carried out. Novel HMW glutenin subunits 1AxTd and 1ByTd were identified. The nucleotide sequences of 1AxTd and 1ByTd genes were detected (access codes in the GenBank are MH475136 and MG897125, respectively). The coding sequence of 1AxTd was similar to 1Ax1 of T. aestivum (99,7 %), while the 1ByTd gene sequence was similar to the genes of other T. dicoccoides samples (98,5 and 97,6 %). The primary and secondary protein structures of the studied HMW-GS allow us to predict a high contribution of the 1AxTd subunit to the bread-making qualities and the average contribution of 1ByTd thereto. The evaluation of the most important grain quality traits revealed the increased content of protein and gluten, along with the reduced rheological properties of T. dicoccoides K5199 gluten.
Key words: T. dicoccoides, HMW-GS, SDS electrophoresis, sequencing, primary and secondary protein structure, grain quality
E-mail: O.Orlovskaya igc.by
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