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Characterization of high molecular weight glutenin subunits in wild emmer wheat (Triticum dicoccoides)

Orlovskaya O.A., Yatsevich K.K., Vakula S.I., Khotyleva L.V., Kilchevsky A.V.


SUMMARY. The identification and molecular analysis of high molecular weight glutenin subunits (HMW-GS) of the wild emmer wheat T. dicoccoides K5199 were carried out. Novel HMW glutenin subunits 1AxTd and 1ByTd were identified. The nucleotide sequences of 1AxTd and 1ByTd genes were detected (access codes in the GenBank are MH475136 and MG897125, respectively). The coding sequence of 1AxTd was similar to 1Ax1 of T. aestivum (99,7 %), while the 1ByTd gene sequence was similar to the genes of other T. dicoccoides samples (98,5 and 97,6 %). The primary and secondary protein structures of the studied HMW-GS allow us to predict a high contribution of the 1AxTd subunit to the bread-making qualities and the average contribution of 1ByTd thereto. The evaluation of the most important grain quality traits revealed the increased content of protein and gluten, along with the reduced rheological properties of T. dicoccoides K5199 gluten.

Key words: T. dicoccoides, HMW-GS, SDS electrophoresis, sequencing, primary and secondary protein structure, grain quality

Tsitologiya i Genetika 2020, vol. 54, no. 3, pp. 28-36

  • Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk

E-mail: O.Orlovskaya

Orlovskaya O.A., Yatsevich K.K., Vakula S.I., Khotyleva L.V., Kilchevsky A.V. Characterization of high molecular weight glutenin subunits in wild emmer wheat (Triticum dicoccoides), Tsitol Genet., 2020, vol. 54, no. 3, pp. 28-36.

In "Cytology and Genetics":
O. A. Orlovskaya, K. K. Yatsevich, S. I. Vakula, L. V. Khotyleva & A. V. Kilchevsky Characterization of High Molecular Weight Glutenin Subunits in Wild Emmer Wheat (Triticum dicoccoides), Cytol Genet., 2020, vol. 54, no. 3, pp. 199205
DOI: 10.3103/S009545272003010X


1. Goncharov, N.P., Kondratenko, E.Ya., Wheat origin, domestication and evolution, Vestn.VOGiS, 2008, vol. 12, no. 1/2, p. 15979.

2. Ozkan, H., Willcox, G., Graner, A., Salamini, F., and Kilian, B., Geographic distribution and domestication of wild emmer wheat (Triticum dicoccoides), Genet. Resour. Crop Evol., 2011, vol. 58, no. 1, p. 1153.

3. Cakmak, I., Torun, A., Millet, E., Feldman, M., Fahima, T., Korol, A.B., Nevo, E., Braun, H.J., and Ozkan, H., Triticum dicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat, Soil Sci. Plant Nutr., 2004, vol. 50, no. 7, p. 104754.

4. Jiang, Z.L., Wu, B.H., Wang, Z.Z., Hu, J.L., Yuan, J., Chen, H.L., Liu, J., Zheng, Y.L., and Liu, D.C., Enriching novel Glu-Ax alleles and significantly strengthening gluten properties of common wheat through wide hybridization with wild emmer, J. Cereal Sci., 2017, vol. 76, p. 2719.

5. Ribeiro, M., Miranda, J., and Branlard, G., One hundred years of grain omics: identifying the glutens that feed the world, J. Proteome Res., 2013, vol. 12, no. 11, pp. 47024716.

6. Payne, P.I., Lawrence, G.J., Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat, Cereal Res. Com., 1983, vol. 11, no. 1, p. 2935.

7. Sun, M., Yan, Y., Jiang, Y., Xiao, Y., Hu, Y., Cai, M., Li, Y., Hsam, S.L.K., and Zeller, F.J., Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccum L.), Hereditas, 2004, vol. 141, no. 1, pp. 4654.

8. Payne, P.I., Nightingale, M.A., Krattiger, A.F., and Holt, L.M., The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties, J. Sci. Food Agric., 1987, vol. 40, no. 1, pp. 5165.

9. Liang, D., Tang, J., Peca, R.J., Singh, R., He, X., Shen, X., Yao, D., Xia, X., and He, Z., Characterization of CIMMYT bread wheats for high- and low-molecular weight glutenin subunits and other quality-related genes with SDS-PAGE, RP-HPLC and molecular markers, Euphytica, 2010, vol. 172, no. 2, pp. 235250.

10. Shewry, P.R., Gilbert, S.M., Savage, A.W.J., Tatham, A.S., Wan, Y.F., Belton, P.S., Wellner, N., DOvidio, R., Bekees, F., and Halford, N.G., Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum) which is associated with poor end use properties, Theor. Appl. Genet., 2003, vol. 106, no. 4, pp. 744750.

11. Santagati, V.D., Sestili, F., Lafiandra, D., DOvidio, R., Rogniaaux, H., and Masci, S., Characterization of durum wheat high molecular weight glutenin subunits Bx20 and By20 sequences by a molecular and proteomic approach, J. Mass Spectrom., 2016, vol. 51, no. 7, p. 5127.

12. Branlard, G., Dardevet, M., Amiour, N., and Igrejas, G., Allelic diversity of HMW and LMW glutenin subunits and omega gliadins in French bread wheat (Triticum aestivum L.), Genet. Resour. Crop Evol., 2003, vol. 50, no. 7, pp. 669679. doi 10. 1023/A:1025077005401

13. Li, Y., Zhou, R., Branlard, G., and Jia, J., Development of introgression lines with 18 alleles of glutenin subunit and evaluation of the effects of various alleles on quality related traits in wheat (Triticum aestivum L.), J. Cereal Sci., 2010, vol. 51, no. 1, pp. 127133.

14. Novoselskaya-Dragovich, A.Yu., Genetics and genomics of wheat: storage proteins, ecological plasticity, and immunity, Russ. J. Genet., 2015, vol. 51, no. 5, pp. 476490.

15. Caballero, L., Martin, L.M., and Alvarez, J.B., Allelic variation for the high- and low-molecular-weight glutenin subunits in wild diploid wheat (Triticum urartu) and its comparison with durum wheat, Aust. J. Agr. Res., 2008, vol. 59, pp. 906910.

16. Lua, C.M., Yanga, W.Y., Zhanga, W.J., and Lua, B.-R., Identification of SNPs and development of allelic specific PCR markers for high molecular weight glutenin subunit Dtx1.5 from Aegilops tauschii through sequence characterization, J. Cereal Sci., 2005, vol. 41, pp. 1318.

17. Margiotta, B., Colaprici, G., and Urbano, M., Polymorphism of high Mr glutenin subunits in wild emmer Triticum turgidum subsp. dicoccoides: chromatographic, electrophoretic separation and PCR analysis of their encoding genes, Genet. Resour. Crop Evol., 2014, vol. 61, no. 2, p. 33143.

18. Liu, Z., Yan, Z., Wan, Y., Liu, K., Zheng, Y., and Wang, D., Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species, Theor. Appl. Genet., 2003, vol.106, no. 8, pp. 13681378.

19. Singh, N.K., Shepherd, K.W., and Cornish, G.B., A simplified SDS-PAGE procedure for separating LMW subunits of glutenin, J. Cereal Sci., 1991, vol. 14, no. 3, pp. 203208.

20. Laemmli, U.K, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, no. 5259, pp. 680685.

21. Kumar, T.A., CFSSP: Chou and Fasman secondary structure prediction server, Wide Spectrum, 2013, vol. 1, no. 9, pp. 1519.

22. Tamura, K., Dudley, J., Nei, M., and Kumar, S., MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0, Mol. Biol. Evol., 2007, vol. 24, no. 8, pp. 15961599.

23. Dobrotvorskaya, T.V. and Martynov, S.P., Analysis of diversity of Russian and Ukrainian bread wheat (Triticum aestivum L.) cultivars for high-molecular-weight glutenin subunits, Russ. J. Genet., 2011, vol. 47. no. 7, p. 799812.

24. Ribeiro, M., Bancel, E., Faye, A., Dardevet, M., Ravel, C., Branlard, G., and Igrejas, G., Proteogenomic characterization of novel x-type high molecular weight glutenin subunit 1Ax1.1, Int. J. Mol. Sci., 2013, vol. 14, no. 3, p. 565067.

25. Shewry, P.R. and Tatham, A.S., Disulphide bonds in wheat gluten proteins, J. Cereal Sci., 1997, vol. 25, pp. 207227.

26. Wieser, H. and Zimmermann, G., Importance of amounts and proportions of high molecular weight subunits of glutenin for wheat quality, Eur. Food Res. Technol., 2000, vol. 210, no. 5, pp. 324330. doi 10.1007/s002170050558

27. Jin, M., Xie, Z., Li, J., Jiang, S., Ge, P., Subburaj, S., Li, X., Zeller, F.J., Hsam, S.L.K., and Yan, Y., Identification and molecular characterization of HMW glutenin subunit 1By16* in wild emmer, J. Appl. Genet., 2012, vol. 53, no. 3, pp. 24958.

28. Shewry, P.R., Halford, N.G., and Tatham, A.S., High molecular weight subunits of wheat glutenin, J. Cereal Sci., 1992, vol. 15, pp. 105120.

29. Utebayev, M., Dashkevich, S., Kunanbayev, K., Bome, N., Sharipova, B., and Shavrucov, Yu., Genetic polymorphism of glutenin subunits with high molecular weight and their role in grain and dough qualities of spring bread wheat (Triticum aestivum L.) from Northern Kazakhstan, Acta Physiol. Plant., 2019, vol. 41, p. 71.

30. He, Z.H., Liu, I., Xia, X.C., Liu, J.J., and Pena, R.J., Composition of HMW and LMW glutenin subunits and their effects on dough properties, pan bread, and noodle quality on Chinese bread wheats, Cereal Chem., 2005, vol. 82, no. 4, p. 34550.

31. Guo, X.H., Hu, J.L., Wu, B.H., Wang, Z.Z., Wang, D., Liu, D.C., and Zheng, Y.L., Special HMW-GSs and their genes of Triticum turgidum subsp. dicoccoides accession D141 and the potential utilization in common wheat, Genet. Resour. Crop. Evol., 2016, vol. 63, no. 5, p. 83344.

32. Zhang, D., Yuan, Y., Su, Y., and Li, S., Analysis of dough rheological property and gluten quality characteristics in wild emmer wheat (Triticum dicoccoides (Korn. ex Asch. et Graebn.) Schweinf.), Genet. Resour. Crop Evol., 2016, vol. 63, no. 4, pp. 675683.

33. Rasheed, A., Xia, X.C., Yan, Y.M., Appels, R., Mahmood, T., and He, Z.H., Wheat seed storage proteins: advances in molecular genetics, diversity and breeding applications, J. Cereal Sci., 2014, vol. 60, no. 1, pp. 1124.

34. Obukhova L.V., Shumny V.K., Composition of high molecular weight glutenin subunits in common wheat varieties and promising lines, Russ. J. Genet., 2018, vol. 54, no. 3, p. 30513.

35. Barak, S., Mudgil, D., and Khatkar, B.S., Biochemical and functional properties of wheat gliadins: a review, Crit. Rev. Food Sci. Nutr., 2015, vol. 55, no. 3, pp. 357368.

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