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Метаболічна інженерія штамів-продуцентів лізину Corynebacterium glutamicum

Андріяш Г.С., Секан О.С., Тігунова О.О., Блюм Я.Б., Шульга С.М.

Огляд 




Огляд присвячено аналізу сучасних досягнень метаболічної інженерії Corynebacterium glutamicum для продукування лізину. Розглянуто ключові гени біосинтезу лізину у C. glutamicum та шляхи створення нових генетично модифікованих штамів. Описана роль різних плазмід, конструювання векторних касет та види промоторів для регуляції експресії генів у C. glutamicum. Наведено інформацію з використання вуглецевмісних субстратів (гексоз, пентоз, молочної кислоти, манітолу) для виробництва лізину. Розглянуто можливості використання технології CRISPR в генетичній інженерії C. glutamicum. Генетичні зміни C. glutamicum дозволили використання альтернативних субстратів та сприяли підвищенню рівня накопичення лізину в культуральній рідині. Узагальнено дані, які можуть слугувати для створення нових штамів-надпродуцентів лізину.

РЕЗЮМЕ. Обзор посвящен анализу современных достижений метаболической инженерии Corynebacterium glutamicum для накопления лизина. Рассмотрены ключевые гены биосинтеза лизина в C. glutamicum и пути создания новых генетически модифицированных штаммов. Описана роль различных плазмид, конструирование векторных кассет и виды промоторов для регуляции экспрессии генов в C. glutamicum. Приведена информация по использованию углеродсодержащих субстратов (гексоз, пентоз, молочной кислоты, маннитола) для производства лизина. Рассмотрены возможности использования технологии CRISPR в генетической инженерии C. glutamicum. Генетические изменения C. glutamicum позволили использование альтернативных субстратов и способ-ствовали повышению уровня накопления лизина в культуральной жидкости. Обобщены данные, которые могут служить для создания новых штаммов-суперпродуцентов лизина.

Ключові слова: Corynebacterium glutamicum, штам-продуцент, мікробіологічний синтез, лізин, метаболічна інженерія

Цитологія і генетика 2020, том 54, № 2, C. 61-73

  1. ДУ «Інститут харчової біотехнології та геноміки НАН України», Київ, Україна
  2. Технологічний університет Люлеа, Департамент технології та дослідження деревини, Скеллефтеа, Швеція

E-mail: Shulga5 i.ua, cellbio cellbio.freenet.viaduk.net

Андріяш Г.С., Секан О.С., Тігунова О.О., Блюм Я.Б., Шульга С.М. Метаболічна інженерія штамів-продуцентів лізину Corynebacterium glutamicum, Цитологія і генетика., 2020, том 54, № 2, C. 61-73.

В "Cytology and Genetics". Якщо тільки можливо, цитуйте статтю по нашій англомовній версії:
G. S. Andriiash, O. S. Sekan, O. O. Tigunova, Ya. B. Blume & S. M. Shulga Metabolic Engineering of Lysine Producing Corynebacterium glutamicum Strains, Cytol Genet., 2020, vol. 54, no. 2, pp. 137–146
DOI: 10.3103/S0095452720020024


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