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Metabolic engineering of lysine producing strains Corynebacterium glutamicum

Andriiash G.S., Sekan .S., Tigunova .., Blume Ya.B., Shulga S..


SUMMARY. The review is devoted to the analysis of the current achievements of the metabolic engineering of Cory-nebacterium glutamicum for the production of lysine. Key genes of lysine biosynthesis in C. glutamicum and ways of creating new genetically modified strains are considered. The role of different plasmids, vector cassettes and promoter types for the regulation of gene expression in C. glutamicum is described. Information is provided on the use of carbon-containing substrates (hexose, pentose, lactic acid, mannitol) for the production of lysine. Possibilities of using CRISPR technology in genetic engineering of C. glutamicum are considered. Genetic changes in C. glutamicum allowed the use of alternative substrates and contributed to the increase of lysine accumulation in the culture fluid. The data that may serve to create new lysine overproduction strains are summarized.

Tsitologiya i Genetika 2020, vol. 54, no. 2, pp. 61-73

  1. SE Institute for Food Biotechnology and Genomics of National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. Division of Wood Science and Engineering, Department of Engineering Sciences and Mathematics, Lulea University of Technology, Skelleftea, Sweden

E-mail: Shulga5, cellbio

Andriiash G.S., Sekan .S., Tigunova .., Blume Ya.B., Shulga S.. Metabolic engineering of lysine producing strains Corynebacterium glutamicum, Tsitol Genet., 2020, vol. 54, no. 2, pp. 61-73.

In "Cytology and Genetics":
G. S. Andriiash, O. S. Sekan, O. O. Tigunova, Ya. B. Blume & S. M. Shulga Metabolic Engineering of Lysine Producing Corynebacterium glutamicum Strains, Cytol Genet., 2020, vol. 54, no. 2, pp. 137146
DOI: 10.3103/S0095452720020024


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