Metabolic engineering of lysine producing strains Corynebacterium glutamicum

References

1. Becker, J. and Wittmann, C., Industrial microorganisms: Corynebacterium glutamicum, in Industrial Biotechnology, Wittmann, C. and Liao, J.C., Eds., Germany, Weinheim, Wiley-VCH, 2017, pp. 183–203. https://doi.org/10.1002/9783527807796.ch6

2. Andriiash, G.S., Zabolotna, G.M., Bondarenko, V.S., and Shulga, S.M., Gene 16S rRNA sequence phylogenetic analysis of lysine producers, Biotechnol. Acta, 2014, vol. 7, no. 6, pp. 40–45. https://doi.org/10.15407/biotech7.06.040

3. Tigunova, O.O., Andriiash, G.S., Beyko, N.E., and Shulga, S.M., Phylogenrtic analysis of lysine, threonine and butanolstraine-producers, Fact. Exp. Evol. Org., 2017, vol. 21, pp. 288–292.

4. Ma, Q., Zhang, Q., Xu, Q., Zhang, C., Li, Y., Fan, X., Xie, X., and Chen, N., Systems metabolicengineering strategies for the production of amino acids, Synth. Syst. Biotechnol., 2017, no. 2, pp. 87–96. https://doi.org/10.1016/j.synbio.2017.07.003

5. Andriiash, G.S., Zabolotna, G.M., and Shulga, S.M., Regulation and intensification ways of lysine biosynthesis, Microbiol. Biotechnol., 2012, no. 4, pp. 6–17. https://doi.org/10.18524/2307-4663.2012.4(20).90435

6. Andriiash, G.S., Zabolotna, G.M., Tkachenko, A.F., Blume, Ya.B., and Shulga, S.M., Threonine synthesis of Brevibacterium flavum, in Threonine: Food Sources, Functions and Health Benefits, USA: Nova Publ., 2015, pp. 1–26.

7. Buschke, N., Schäfer, R., and Becker, J., and Wittmann, C., Metabolic engineering of industrial platform microorganisms for biorefinery applications—optimization of substrate spectrum and process robustness by rational and evolutive strategies, Bioresour. Technol., 2013, vol. 135, pp. 544–554. https://doi.org/10.1016/j.biortech.2012.11.047

8. Date, M., Itaya, H., Matsui, H., and Kikuchi, Y., Secretion of human epidermal growth factor by Corynebacterium glutamicum,Lett. Appl. Microbiol., 2006, vol. 42, pp. 66–70. https://doi.org/10.1111/j.1472-765X.2005.01802.x

9. Tryfona, T. and Mak, T., Fermentative production of lysine by Corynebacterium glutamicum: transmembrane transport and metabolic flux analysis, Process Biochem., 2005, vol. 40, pp. 499–508. https://doi.org/10.1016/j.procbio.2004.01.037

10. Hermann, T., Industrial production of amino acids by coryneform bacteria, J. Biotechnol., 2003, vol. 104, pp. 155–172. https://doi.org/10.1016/S0168-1656(03)00149-4

11. Koffas, M.A., Jung, G., Yeol., G., and Stephanopoulos, G., Engineering metabolism and product formation in Corynebacterium glutamicum by coordinated gene overexpression, Metab. Eng., 2003, vol. 5, pp. 32–41. https://doi.org/10.1016/S1096-7176(03)00002-8

12. Kromer, J.O., Sorgenfrei, O., Klopprogge, K., Heinzle, E., and Wittmann, C., In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome, J. Bacteriol., 2004, vol. 186, no. 6, pp. 1769–1784. https://doi.org/10.1128/JB.186.6.1769-1784.2004

13. Ohnishi, J., Mitsuhashi, S., Hayashi, M., Ando, S., Yokoi, H., Ochiai, K., and Ikeda, M., A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant, Appl. Microbiol. Biotechnol., 2002, vol. 58, pp. 217–223. https://doi.org/10.1007/s00253-001-0883-6

14. Papagianni, M., Recent advances in engineering the central carbon metabolism of industrially important bacteria, Microb. Cell Factories, 2012, vol. 11, pp. 1–13. https://doi.org/10.1186/1475-2859-11-50

15. Sun, Y., Guo, W., Wang, F., Zhan, C., Yang, Y., Liu, X., and Bai, Z., Transcriptome analysis of Corynebacterium glutamicum in the process of recombinant protein expression in bioreactors, PLoS One, 2017, vol. 12, no. 4, pp. 1–18. https://doi.org/10.1371/journal.pone.0174824

16. Eikmanns, B.J., Central metabolism: tricarboxylic acid cycle and anaplerotic reactions, in Handbook of Corynebacterium glutamicum, Eggelingand, L. and Bott, M., Eds., Boca Raton, FL: CRC Press, 2005, pp. 241–276.

17. Baritugo, K.A., Kim, H.T., David, Y., Choi, J.-L., Hong, S.H., and Jeong, K.J., Metabolic engineering of Corynebacterium glutamicum for fermentative production of chemicals in biorefinery, Appl. Microbiol. Biotechnol., 2018, vol. 102, pp. 3915–3937. https://doi.org/10.1007/s00253-018-8896-6

18. Nakamura, Y., Nishio, Y., Ikeo, K., and Gojobori, T., The genome stability in Corynebacterium species due to lack of the recombinational repair system, Gene, 2003, vol. 317, pp. 149–155. https://doi.org/10.1016/S0378-1119(03)00653-X

19. Leßmeier, L. and Wendisch, V.F., Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum,BMC Microbiol., 2015, vol. 15, no. 216. https://doi.org/10.1186/s12866-015-0558-6

20. Nesvera, J. and Patek, M., Tools for genetic manipulations in Corynebacterium glutamicum and their applications, Appl. Microbiol. Biotechnol., 2011, vol. 90, no. 5, pp. 1641–1654. https://doi.org/10.1007/s00253-011-3272-9

21. Liebl, W., Sinskey, A.J., and Schleifer, K.H., Expression, secretion, and processing of staphylococcal nuclease by Corynebacterium glutamicum,J. Bacteriol., 1992, vol. 174, no. 6, pp. 1854–1861. https://doi.org/10.1128/jb.174.6.1854-1861.1992

22. Eikmanns, B.J., Thum-Schmitz, N., Eggeling, L., Ludtke, K.U., and Sahm, H. Nucleotide sequence,expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase, Microbiology, 1994, vol. 140, no. 8, pp. 1817–1828. https://doi.org/10.1099/13500872-140-8-1817

23. Jakoby, M., Ngouoto-Nkili, C.E., and Burkovski, A., Construction and application of new Corynebacterium glutamicum vectors, Biotechnol. Techniques, 1999, vol. 13, p. 437. https://doi.org/10.1023/A:1008968419217

24. Peters-Wendisch, P.G., Schiel, B., Wendisch, V.F., Katsoulidis, E., Mockel, B., Sahm, H., and Eikmanns, B.J., Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum,J. Mol. Microbiol. Biotechnol., 2001, vol. 3, pp. 295–300.

25. Yasuda, K., Jojima, T., Suda, M., Okino, S., Inui, M., and Yukawa, H., Analyses of the acetate-producing pathways in Corynebacterium glutamicum under oxygen-deprived conditions, Appl. Microbiol. Biotechnol., 2008, vol. 77, no. 4, pp. 853–860. https://doi.org/10.1007/s00253-007-1199-y

26. Sato, H., Orishimo, K., Shirai, T., Hirasawa, T., Nagahisa, K., Shimizu, H., and Wachi, M., Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum,J. Biosci. Bioeng., 2008, vol. 106, no. 1, pp. 51–58. https://doi.org/10.1263/jbb.106.51

27. Krause, F.S., Blombach, B., and Eikmanns, B.J., Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production, Appl. Environ. Microbiol., 2010, vol. 76, no. 24, pp. 8053–8061. https://doi.org/10.1128/AEM.01710-10

28. Yang, Y.F., Zhang, J.J., Wang, S.H., and Zhou, N.Y., Purification and characterization of the ncgli2923-encoded 3-hydroxybenzoate 6-hydroxylase from Corynebacterium glutamicum,J. Basic Microbiol., 2010, vol. 50, no. 6, pp. 599–604. https://doi.org/10.1002/jobm.201000053

29. Santamaria, R., Gil, J., Mesas, J., and Martin, J., Characterization of an endogenous plasmid and development of cloning vectors and a transformation system in Brevibacterium lactofermentum,J. Gen. Microbiol., 1984, vol. 130, pp. 2237–2246. https://doi.org/10.1099/00221287-130-9-2237

30. Sonnen, H., Thierbach, G., Kautz, S., Kalinowski, J., Schneider, J., Puhler, A., and Kutzner, H.J., Characterization of pGA1, a new plasmid from Corynebacterium glutamicum LP-6, Gene, 1991, vol. 107, no. 1, pp. 69–74. https://doi.org/10.1016/0378-1119(91)90298-P

31. Ozaki, A., Katsumata, R., Oka, T., and Furuya, A., Functional expression of the genes of Escherichia coli in gram-positive Corynebacterium glutamicum,Mol. Gen. Genet., 1984, vol. 196, no. 1, pp. 175–178. https://doi.org/10.1007/BF00334113

32. Kirchner, O. and Tauch, A., Tools for genetic engineering in the amino acid-producing bacterium Corynebacterium glutamicum,J. Biotechnol., 2003, vol. 104, pp. 287–299. https://doi.org/10.1016/S0168-1656(03)00148-2

33. Patek, M., Holatko, J., Busche, B., Kalinowski, J., and Nesvera, J., Corynebacterium glutamicum promoters: a practical approach, Microb. Biotechnol., 2013, vol. 6, no. 2, pp. 103–117.

34. Studier, F.W. and Moffatt, B.A., Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes, J. Mol. Biol., 1986, vol. 189, no. 1, pp. 113–130. https://doi.org/10.1016/0022-2836(86)90385-2

35. Kortmann, M., Kuhl, V., Klaffl, S., and Bott, M., A chromosomally encoded T7 RNA polymerase-dependent gene expression system for Corynebacterium glutamicum: construction and comparative evaluation at the single-cell level, Microb. Biotechnol., 2015, vol. 8, no. 2, pp. 253–265. https://doi.org/10.1111/1751-7915.12236

36. Peters-Wendisch, P.G., Schiel, B., Wendisch, V.F., Katsoulidis, E., Mockel, B., Sahm, H., and Eikmanns, B.J., Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum,J. Mol. Microbiol. Biotechnol., 2001, vol. 3, no. 2, pp. 295–300.

37. Nakunst, D., Larisch, C., Hüser, A.T., Tauch, A., Puhler, A., and Kalinowski, J., The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes, J. Bacteriol., 2007, vol. 189, no. 13, pp. 4696–4707. https://doi.org/10.1128/JB.00382-07

38. Ehira, S., Teramoto, H., Inui, M., and Yukawa, H., Regulation of Corynebacterium glutamicum heat shock response by the extracytoplasmic-function sigma factor SigH and transcriptional regulators HspR and HrcA, J. Bacteriol., 2009, vol. 191, no. 9, pp. 2964–2972. https://doi.org/10.1128/JB.00112-09

39. Larisch, C., Nakunst, D., Huser, A.T., Tauch, A., and Kalinowski, J., The alternative sigma factor SigBof Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase, BMC Genomics, 2007, vol. 8. https://doi.org/10.1186/1471-2164-8-4

40. Busche, T., Silar, R., Picmanova, M., Patek, M., and Kalinowski, J., Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum,BMC Genomics, 2012, vol. 13, no. 445. https://doi.org/10.1186/1471-2164-13-445

41. Zhang, Y., Shang, LaiSh., Zhang, G., Liang, Y., and Wen, T., Development and application of an arabinose-inducible expression system by facilitating inducer uptake in Corynebacterium glutamicum,Appl. Environ. Microbiol., 2012, vol. 78, no. 16, pp. 5831–5838. https://doi.org/10.1128/AEM.01147-12

42. Yim, S.S., An S.J., Kang M., Lee J., and Jeong K.J. Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum,Biotechnol. Bioeng., 2013, vol. 110, no. 11, pp. 2959–2969. https://doi.org/10.1002/bit.24954

43. Rytter, J.V., Helmark, S., Chen, J., Lezyk, M.J., Solem, C., and Jensen, P.R., Synthetic promoter libraries for Corynebacterium glutamicum,Appl. Microbiol. Biotechnol., 2014, vol. 98, no. 6, pp. 2617–2623. https://doi.org/10.1007/s00253-013-5481-x

44. Becker, J., Zelder, O., Hafner, S., Schroder, H., and Wittmann, C., From zero to hero-design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production, Metab. Eng., 2011, vol. 13, no. 2, pp. 159–168. https://doi.org/10.1016/j.ymben.2011.01.003

45. Ooyen, J., Noack, S., Bott, M., Reth, A., and Eggeling, L., Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity, Biotechnol. Bioeng., 2012, vol. 109, no. 8, pp. 2070–2081. https://doi.org/10.1002/bit.24486

46. Hänssler, E., Muller, T., Palumbo, K., Patek, M., Brocker, M., Kramer, R., and Burkovski, A., A game with many players: control of gdh transcription in Corynebacterium glutamicum,J. Biotechnol., 2009, vol. 142, pp. 114–122. https://doi.org/10.1016/j.jbiotec.2009.04.007

47. Tanaka, Y., Okai, N., Teramoto, H., Inui, M., and Yukawa, H., Regulation of the expression of phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) genes in Corynebacterium glutamicum R, Microbiology, 2008, vol. 154, pp. 264–274. https://doi.org/10.1099/mic.0.2007/008862-0

48. Gaigalat, L., Schluter, J.P., Hartmann, M., Mormann, Tauch, A., Puhler, A., and Kalinowski, J., The DeoR-type transcriptional regulator SugR acts as a repressor for genes encoding the phosphoenol pyruvate: sugar phosphotransferase system (PTS) in Corynebacterium glutamicum, BMC Mol. Biol., 2007, vol. 8. https://doi.org/10.1186/1471-2199-8-104

49. Ikeda, M., Ohnishi, J., Hayashi, M., and Mitsuhashi, S., A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient L-lysine production, J. Ind. Microbiol. Biotechnol., 2006, vol. 33, pp. 610–615. https://doi.org/10.1007/s10295-006-0104-5

50. Hayashi, M., Ohnishi, J., Mitsuhashi, S., Yonetani, Y., Hashimoto, S., and Ikeda, M., Transcriptome analysis reveals global expression changes in an industrial l-lysine producer of Corynebacterium glutamicum,Biosci. Biotechnol. Biochem., 2006, vol. 70, pp. 546–550. https://doi.org/10.1271/bbb.70.546

51. Brinkrolf, K., Schröder, J., Pühler, A., and Tauch, A., The transcriptional regulatory repertoire of Corynebacterium glutamicum: reconstruction of the network controlling pathways involved in lysine and glutamate production, J. Biotech., vol. 149, no. 3, pp. 173–182. https://doi.org/10.1016/j.jbiotec.2009.12.004

52. Binder, B., Siedler, S., Marienhagen, J., Bott, M., and Eggeling, L., Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation, Nucleic Acids Res., 2013, vol. 41, no. 12, pp. 6360–6369. https://doi.org/10.1093/nar/gkt312

53. Kortmann, M., Mack, C., Baumgar, M., and Bott, M., Pyruvate carboxylase variants enabling improved lysine production from glucose identified by biosensor-based high-throughput fluorescence-activated cell sorting screening, ACS Synth. Biol., 2019, vol. 8, no. 2, pp. 274–281. https://doi.org/10.1021/acssynbio.8b00510

54. Wendisch, V.F., Microbial production of amino acids and derived chemicals: synthetic biology approaches to strain development, Curr. Opin. Biotechnol., 2014, vol. 30, pp. 51–58. https://doi.org/10.1016/j.copbio.2014.05.004

55. Chen, Z., Rappert, S., and Zeng, A.P., Rational design of allosteric regulation of homoserine dehydrogenase by a non-natural inhibitor L-lysine, ACS Synth. Biol., 2015, vol. 4, no. 2, pp. 126–131. https://doi.org/10.1021/sb400133g

56. Zhou, L.B. and Zeng, A.P., Exploring lysine riboswitch for metabolic flux control and improvement of L-lysine synthesis in Corynebacterium glutamicum,ACS Synth. Biol., 2015, no. 4, pp. 729–734. https://doi.org/10.1021/sb500332c

57. Hoffmann, S.L., Jungmann, L., Schiefelbein, S., Peyriga, L., Cahoreau, E., Portais, J.C., Becker, J., and Wittmann, C., Lysine production from the sugar alcohol mannitol: design of the cell factory Corynebacterium glutamicum SEA-3 through integrated analysis and engineering of metabolic pathway fluxes, Metab. Eng. 2018, vol. 47, pp. 475–487. https://doi.org/10.1016/j.ymben.2018.04.019

58. Schneider, J., Niermann, K., and Wendisch, V.F., Production of the amino acids L-glutamate, L-lysine, L-ornithine and L-arginine from arabinose by recombinant Corynebacterium glutamicum,J. Biotechnol., 2011, vol. 154, nos. 2–3, pp. 191–198. https://doi.org/10.1016/j.jbiotec.2010.07.009

59. Buschke, N., Schröder, H., and Wittmann, C., Metabolic engineering of Corynebacterium glutamicum for production of 1,5-diaminopentane from hemicellulose, Biotechnol. J., 2011, vol. 6, no. 3, pp. 306–317. https://doi.org/10.1002/biot.201000304

60. Kawaguchi, H., Vertes, A.A., Okino, S., Inui, M., and Yukawa, H., Engineering of a xylose metabolic pathway in Corynebacterium glutamicum,Appl. Environ. Microbiol., 2006, vol. 72, no. 5, pp. 3418–3428. https://doi.org/10.1128/AEM.72.5.3418-3428.2006

61. Ryu, S. and Karim, M.N., A whole cell biocatalyst for cellulosic ethanol production from dilute acid-pretreated corn stover hydrolyzates, Appl. Microbiol. Biotechnol., 2011, vol. 91, no. 3, pp. 529–542. https://doi.org/10.1007/s00253-011-3261-z

62. Mori, M. and Shiio, I., Pyruvate formation and sugar metabolism in an amino acid-producing bacterium Brevibacterium flavum,Agric. Biol. Chem., 1987, vol. 51, no. 1, pp. 129–138. https://doi.org/10.1271/bbb1961.51.129

63. Parche, S., Burkovski, A., Sprenger, G.A., Weil, B., Kramer, R., and Titgemeyer, F., Corynebacterium glutamicum: a dissection of the PTS, J. Mol. Microbiol. Biotechnol., 2001, vol. 3, no. 3, pp. 423–428.

64. Ikeda, M., Sugar transport systems in Corynebacterium glutamicum: features and applications to strain development, Appl. Microbiol. Biotechnol., 2012, vol. 96, no. 5, pp. 1191–1200. https://doi.org/10.1007/s00253-012-4488-z

65. Neuner, A. and Heinzle, E., Mixed glucose and lactate uptake by Corynebacterium glutamicum through metabolic engineering, Biotechnol. J., 2011, vol. 6, no. 3, pp. 318–329. https://doi.org/10.1002/biot.201000307

66. Ikeda, M., Mizuno, Y., Awane, S., Hayashi, M., Mitsuhashi, S., and Takeno, S., Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum,Appl. Microbiol. Biotechnol., 2011, vol. 90, no. 4, pp. 1443–1451. https://doi.org/10.1007/s00253-011-3210-x

67. Lindner, S.N., Seibold, G.M., Henrich, A., Kramer, R., and Wendisch, V.F., Phosphotransferase system independent glucose utilization in Corynebacterium glutamicum by inositol permeases and glucokinases, Appl. Environ. Microbiol., 2011, vol. 77, no. 11, pp. 3571–3581. https://doi.org/10.1128/AEM.02713-10

68. Tan, J.M., Wong, E.S., Kirkpatrick, D.S., Pletnikova, O., Ko, H.S., Tay, S.P., Ho, M.W., Troncoso, J., Gygi, S.P., Lee, M.K., Dawson, V.L., Dawson, T.M., and Lim, K.L., Lysine 63-linked ubiquitination promotes the formation and autophagic clearance of protein inclusions associated with neurodegenerative diseases, Hum. Mol. Genet., 2008, vol. 17, no. 3, pp. 431–439. https://doi.org/10.1093/hmg/ddm320

69. Kawaguchi, A., Ikawa, T., and Kasukawa, T., Ueda, H.R., Kurimoto, K., Saitou, M., and Matsuzaki, F., Single cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis, Development, 2008, vol. 135, no. 18, pp. 3113–3124. https://doi.org/10.1242/dev.022616

70. Meiswinkel, T.M., Gopinath, V., Lindner, S.N., Nampoothiri, K.M., and Wendisch, V.F., Accelerated pentose utilization by Corynebacterium glutamicum for accelerated production of lysine, glutamate, ornithine and putrescine, Microb. Biotechnol., 2013, vol. 6, no. 2, pp. 131–140.

71. Radek, A., Krumbach, K., Gaetgens, J., Wendisch, V.F., Wiechert, W., Bott, M., Noack, S., and Marienhagen, J., Engineering of Corynebacterium glutamicum for minimized carbon loss during utilization of D-xylose containing substrates, J. Biotechnol., 2014, vol. 192, pp. 156–160. https://doi.org/10.1016/j.jbiotec.2014.09.026

72. Marin, K. and Krämer, R., Amino acid transport systems in biotechnologically relevant bacteria, in Amino Acid Biosynthesis—Pathways, Regulation and Metabolic Engineering, Wendisch, V.F., Ed., Microbiology Monographs, Berlin: Springer, 2007, vol. 5, pp. 289–325. https://doi.org/10.1007/7171_2006_069

73. Eggeling, L., Exporters for production of amino acids and other small molecules, Adv. Biochem. Eng. Biotechnol., 2017, vol. 159, pp. 199–225. https://doi.org/10.1007/10_2016_32

74. Blombach, B., Hans, S., and Bathe, B., and EikmannsB.J., Acetohydroxyacid synthase, a novel target for improvement of L-lysine production by Corynebacterium glutamicum,Appl. Environ. Microbiol., 2009, vol. 75, no. 2, pp. 419–427. https://doi.org/10.1128/AEM.01844-08

75. Unthan, S., Radek, A., Wiechert, W., Oldiges, M., and Noack, S., Bioprocess automation on a mini pilot plant enables fast quantitative microbial phenotyping, Microb. Cell Factories, 2015, vol. 14, p. 32. https://doi.org/10.1186/s12934-015-0216-6

76. Pérez-García, F., Peters-Wendisch, P., and Wendisch, V.F., Engineering Corynebacterium glutamicum for fast production of L-lysine and L-pipecolic acid, Appl. Microbiol. Biotechnol., 2016, vol. 100, no. 18, pp. 8075–8090. https://doi.org/10.1007/s00253-016-7682-6

77. Henke, N.A., Wiebe, D., Perez-Garcia, F., Peters-Wendisch, P., and Wendisch, V.F., Coproduction of cell-bound and secreted value-added compounds: simultaneous production of carotenoids and amino acids by Corynebacterium glutamicum,Bioresour. Technol., 2018, vol. 247, pp. 744–752. https://doi.org/10.1016/j.biortech.2017.09.167

78. Kind, S., Neubauer, S., Becker, J., Yamamoto, M., Volkert, M., Abendroth, G.V., and Zelder, O., and WittmannC. From zero to hero-production of biobased nylon from renewable resources using engineered Corynebacterium glutamicum,Metab. Eng., 2014, vol. 25, pp. 113–123. https://doi.org/10.1016/j.ymben.2014.05.007

79. Rohles, C.M., Gießelmann, G., Kohlstedt, M., Wittman, Ch., and Becker, J., Systems metabolic engineering of Corynebacterium glutamicum for the production of the carbon-5 platform chemicals 5-aminovalerate and glutarate, Microb. Cell Fact., 2016, vol. 15, no. 1, pp. 154–167. https://doi.org/10.1186/s12934-016-0553-0

80. Pérez-García, F., Risse, J.M., Friesh, K., and Wendisch, V.F., Fermentative production of L-pipecolic acid from glucose and alternative carbon sources, Biotech. J., 2017, vol. 12, no. 7. https://doi.org/10.1002/biot.201600646

81. Goh, C.S. and Lee, K.T., Conceptual macroalgae-based third-generation bioethanol (TGB) biorefinery in Sabah. Malaysia as an underlay for renewable and sustainable development, Renew. Sustain. Energy Rev., 2010, vol. 14, pp. 842–848. https://doi.org/10.1016/j.rser.2009.10.001

82. Wang, X., Liu, X., and Wang, G., Two-stage hydrolysis of invasive algal feedstock for ethanol fermentation, J. Integr. Plant Biol., 2011, vol. 53, no. 3, pp. 246–252. https://doi.org/10.1111/j.1744-7909.2010.01024.x

83. Peng, X., Okai, N., Vertes, A.A., Inatomi, K., Inui, M., and Yukawa, H., Characterization of the mannitol catabolic operon of Corynebacterium glutamicum,Appl. Microbiol. Biotechnol., 2011, vol. 91, no. 5, pp. 1375–1387. https://doi.org/10.1007/s00253-011-3352-x

84. Takeno, S., Murata, R., Kobayashi, R., Mitsuhashi, S., and Ikeda, M., Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production, Appl. Environ. Microbiol., 2010, vol. 76, no. 21, pp. 7154–7160. https://doi.org/10.1128/AEM.01464-10

85. Bommareddy, R.R., Chen, Z., Rappert, S., and Zeng, A.P., A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase, Metab. Eng., 2014, vol. 25, pp. 30–37. https://doi.org/10.1016/j.ymben.2014.06.005

86. Eggeling, L. and Bott, M., A giant market and a powerful metabolism: L-lysine provided by Corynebacterium glutamicum,Appl. Microbiol. Biotechnol., 2015, vol. 99, no. 8, pp. 3387–3394.

87. Marx, A., Hans, S., Mockel, B., Bathe, B., de Graaf, A.A., McCormack, A.C., Stapleton, C., Burke, K., O’Donohue, M., and Dunican, L.K., Metabolic phenotype of phosphoglucose isomerase mutants of Corynebacterium glutamicum,J. Biotechnol., 2003, vol. 104, pp. 185–197. https://doi.org/10.1016/S0168-1656(03)00153-6

88. Ohnishi, J., Katahira, R., Mitsuhashi, S., Kakita, S., and Ikeda, M., A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum,FEMS Microbiol. Lett., 2005, vol. 242, pp. 265–274. https://doi.org/10.1016/j.femsle.2004.11.014

89. Buchholz, J., Schwentner, A., Brunnenkan, B., Gabris, C., Grimm, S., Gerstmeir, R., Takors, R., Eikmanns Eikmanns, B.J., and Blombach, B., Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovalerate, Appl. Environ. Microbiol., 2013, vol. 79, no. 18, pp. 5566–5575. https://doi.org/10.1128/AEM.01741-13

90. Becker, J., Klopprogge, C., Herold, A., Zelder, O., Bolten, C.J., and Wittmann, C., Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum-over expression and modification of G6P dehydrogenase, J. Biotechnol., 2007, vol. 132, no. 2, pp. 99–109. https://doi.org/10.1016/j.jbiotec.2007.05.026

91. Becker, J. and Wittmann, C., Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and healthcare products, Angew. Chem., Int. Ed., 2015, vol. 54, pp. 3328–3350. https://doi.org/10.1002/anie.201409033

92. Cleto, S., Jensen, J.V., Wendisch, V.F., and Lu, T.K., Corynebacterium glutamicum metabolic engineering with CRISPR interference (CRISPRi), ACS Synth. Biol., 2016, no. 5, pp. 375–385. https://doi.org/10.1021/acssynbio.5b00216

93. Park, J., Shin, H., Lee, S.-M., Um, Y., and Woo, H.M., RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its application to industrial strain, Microb. Cell Fact., 2018, vol. 17, no. 4. https://doi.org/10.1186/s12934-017-0843-1

94. Cho, J.S., Choi, K.R., Prabowo, C.P.S., Shin, J.H., Yang, D., Jang, J., and Lee, S.Y., CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum,Metabol. Eng., 2017, no. 42, pp. 157–167. https://doi.org/10.1016/j.ymben.2017.06.010

95. Shuman, S. and Glickman, M.S., Bacterial DNA repair by non-homologous end joining, Nat. Rev. Microbiol., 2007, vol. 5, no. 11, pp. 852–861. https://doi.org/10.1038/nrmicro1768

96. Wang, B., Qitiao, HuQ., Zhang, Y., and Shi, R., Chai, X., Liu, Z., Shang, X., Zhang, Y., and Wen, T., A RecET-assisted CRISPR–Cas9 genome editing in Corynebacterium glutamicum,Microb. Cell Fact., 2018, vol. 17, no. 63, pp. 1–16. https://doi.org/10.1186/s12934-018-0910-2