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Methylation of exogenous promoters regulates soybean isoflavone synthase (GmIFS) transgene in T0 transgenic wheat (Triticum aestivum)

Elseehy Mona Mohamed, El­Shehawi Ahmed Mohamed

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РЕЗЮМЕ. Метилювання ДНК – це одна з епігенетичних стратегій регуляції генів у живих організмах. У цьому дослідженні було проведено вивчення експресії трансгену синтази ізофлавону сої (GmIFS) у Т0 рослинах трансгенної пшениці на рівні РНК та рівні геністину у кінцевому продукті. T0 рослини продемонстрували відмінності експресії GmIFS. Статус метилювання проксимальних послідовностей екзогенних промотерів (35S або олеозин (OL)) у Т0 рослинах досліджували за використання бісульфітного секвенування з метою виявлення їхнього метилювання одночасно з рівнем експресії GmIFS. Результати продемонстрували, що значні експресори GmIFS T0 рослин мали високий рівень метилювання проксимальних послідовностей екзогенного промотера, а також низький рівень експресії метилтрансфераз ДНК (Mets). Відмінності в GmIFS були пов’язані з рівнем метилювання в промотерах 35S або OL. Високий рівень експресії GmIFS мав негативну кореляцію з рівнем метилювання проксимальних ділянок промотера 35S і OL. У промотері 35S рівень метилювання сайтів CpG -56 і -88 суттєво пов’язаний з експресією GmIFS і залучений до регуляції гену GmIFS. У промотері OL сайт CpG може бути залучений до регуляції GmIFS. Експресія Met3 пшениці була різною для різних T0 трансгенних рослин. Можливо, профіль її експресії пояснює регуляцію трансгену GmIFS шляхом метилювання.

Ключові слова: трансгенний, пшениця, епігенетичний, метилювання, промотер, метилтрансфераза ДНК

Цитологія і генетика 2020, том 54, № 3, C. 100-102

  1. Department of Genetics, Faculty of Agriculture, University of Alexandria, Elshatby, Alexandria, Egypt
  2. Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia

E-mail: elshehawi hotmail.com

Elseehy Mona Mohamed, El­Shehawi Ahmed Mohamed Methylation of exogenous promoters regulates soybean isoflavone synthase (GmIFS) transgene in T0 transgenic wheat (Triticum aestivum), Цитологія і генетика., 2020, том 54, № 3, C. 100-102.

В "Cytology and Genetics". Якщо тільки можливо, цитуйте статтю по нашій англомовній версії:
Mona Mohamed Elseehy & Ahmed Mohamed El-Shehawi Methylation of Exogenous Promoters Regulates Soybean Isoflavone Synthase (GmIFS) Transgene in T0 Transgenic Wheat (Triticum aestivum), Cytol Genet., 2020, vol. 54, no. 3, pp. 271–282
DOI: 10.3103/S0095452720030032


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