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Methylation of exogenous promoters regulates soybean isoflavone synthase (GmIFS) transgene in T0 transgenic wheat (Triticum aestivum)

Elseehy Mona Mohamed, ElShehawi Ahmed Mohamed


DNA methylation is one epigenetic strategy for gene regulation in living organisms. In this study, the expression of soybean isoflavone synthase (GmIFS) transgene in T0 transgenic wheat plants was investigated at the RNA and the final product genestin level. T0 plants showed variations in the GmIFS expression. Methylation status of the exogenous promoters (35S or Oleocin (OL)) proximal sequence was investigated in T0 plants using bisulphite sequencing to disclose their methylation in parallel with the GmIFS level of expression. Results concluded that the high GmIFS expressers of T0 plants exhibited high methylation of exogenous promoter proximal sequences as well as low expression of DNA methyltransferases (Mets). Variation in GmIFS was associated with the level of methylation in the 35S or OL promoters. High expression of GmIFS was negatively correlated with methylation level of 35S and OL promoter proximal regions. In 35S promoter, methylation level of the CpG sites 56 and 88 is strongly linked to GmIFS expression and is involved in the regulation of GmIFS gene. In OL promoter, the CpG site could be involved in the regulation of the GmIFS. Wheat Met3 expression varied among T0 transgenic plants. Its expression profile could explain the regulation of GmIFS transgene by methylation.

Key words: Transgenic, Wheat, Epigenetic, Methylation, Promoter, DNA methyltransferase

Tsitologiya i Genetika 2020, vol. 54, no. 3, pp. 100-102

  1. Department of Genetics, Faculty of Agriculture, University of Alexandria, Elshatby, Alexandria, Egypt
  2. Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia

E-mail: elshehawi

Elseehy Mona Mohamed, ElShehawi Ahmed Mohamed Methylation of exogenous promoters regulates soybean isoflavone synthase (GmIFS) transgene in T0 transgenic wheat (Triticum aestivum), Tsitol Genet., 2020, vol. 54, no. 3, pp. 100-102.

In "Cytology and Genetics":
Mona Mohamed Elseehy & Ahmed Mohamed El-Shehawi Methylation of Exogenous Promoters Regulates Soybean Isoflavone Synthase (GmIFS) Transgene in T0 Transgenic Wheat (Triticum aestivum), Cytol Genet., 2020, vol. 54, no. 3, pp. 271282
DOI: 10.3103/S0095452720030032


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