TSitologiya i Genetika 2021, vol. 55, no. 1, 3-12
Cytology and Genetics 2021, vol. 55, no. 1, 1–9, doi: https://www.doi.org/10.3103/S0095452721010035

Intraspecific differentiation of white mistletoe (Viscum album L.) using intron length polymorphism of β-tubulin genes and SSR-analysis

Bilonozhko Yu.O., Rabokon A.M., Postovoitova A.S., Kalafat L.O., Privalikhin S.N., Demkovych A.E., Blume Ya.B., Pirko Ya.V.

  • Institute of Food Biotechnology and Genomics, of NAS of Ukraine, Оsypovskoho str., 2A, Kyiv, 04123, Ukraine

SUMMARY. Based on the analysis of the polymorphism of the length of the first intron of the β-tubulin genes, the difference between two subspecies of mistletoe (V. album spp. austriacum (Wiesb.) and V. album ssp. album L.) was shown. The possibility of using the TBP analysis to determine the sex of mistletoe plants is also shown. It was possible to isolate individual genotypes of white mistletoe based on the results of the SSR analysis. When assessing the effectiveness of using two types of DNA markers, it was shown that TBP analysis is advisable to differentiate between different subspecies of mistletoe, and SSR analysis is used to study genotypic variability within a particular subspecies.

Keywords: Viscum album L., DNA marker, SSR-, TВР-analysis

TSitologiya i Genetika
2021, vol. 55, no. 1, 3-12

Current Issue
Cytology and Genetics
2021, vol. 55, no. 1, 1–9,
doi: 10.3103/S0095452721010035

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