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Development of SSR Markers for Psammochloa villosa (Trin.) Bor (Poaceae), a Dominant Species in the Inner Mongolian Plateau

Liu Y.-P., Liang R.-F., Lv T., Ma Z.-L., Liu T., Liu F., Su X., Harris A.J.

 




We developed a set of SSR markers to investigate the genetic diversity and evolutionary history of Psammochloa villosa (Trin.) Bor, a dominant species of the Inner Mongolian Plateau and adjacent regions. We used sequenced transcriptomes of P. villosa to develop primer pairs for 97 SSR markers. Of these, we successfully amplified 46 (47 %) SSRs and found that eleven were polymorphic among individuals. We tested these eleven SSRs in 122 accessions of P. villosa representing ten populations, and determined that the number of alleles per locus ranged from one to seven, with an average of 2.727. The expected and observed heterozygosity per locus varied from 0 to 0.840 (mean: 0.471) and from 0 to 1.000 (mean: 0.679), respectively. The new SSR markers described in this study will be valuable for evaluating the population genetic structure and history of P. villosa throughout its range and may help to develop hypotheses for understanding the history of its divergence from its closest relatives in the genus Stipa L.

Key words: grass; Illumina; microsatellite marker; orthologous gene; transcriptome

Tsitologiya i Genetika 2021, vol. 55, no. 6, pp. 78-79

  1. Key Laboratory of Medicinal Animal and Plant Resources in the QinghaiTibetan Plateau, School of Life Sciences, Qinghai Normal University, Xining 810008, China
  2. Key Laboratory of Physical Geography and Environmental Process in Qinghai Province, Qinghai Normal University, Xining 810008, China
  3. Key Laboratory of Education Ministry of Earth Surface Processes and Ecological Conservation of the QinghaiTibet Plateau, Qinghai Normal University, Xining 810008, China
  4. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

E-mail: xusu8527972 126.com; aj.harris inbox.com

Liu Y.-P., Liang R.-F., Lv T., Ma Z.-L., Liu T., Liu F., Su X., Harris A.J. Development of SSR Markers for Psammochloa villosa (Trin.) Bor (Poaceae), a Dominant Species in the Inner Mongolian Plateau, Tsitol Genet., 2021, vol. 55, no. 6, pp. 78-79.

In "Cytology and Genetics":
Yu-Ping Liu, Rui-Fang Liang, Ting Lv, Zi-Lan Ma, Tao Liu, Feng Liu, Xu Su & AJ Harris Development of SSR Markers for Psammochloa villosa (Trin.) Bor (Poaceae), a Dominant Species in the Inner Mongolian Plateau, Cytol Genet., 2021, vol. 55, no. 6, pp. 576582
DOI: 10.3103/S0095452721060086


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