TSitologiya i Genetika 2021, vol. 55, no. 4, 77-78
Cytology and Genetics 2021, vol. 55, no. 4, 396–403, doi: https://www.doi.org/10.3103/S0095452721040125

Inflammatory and anti­inflammatory cytokine activity in the cartilage cells of genetically modified mice

Torgomyan A., Saroyan M.

  • Yerevan State Medical University after M. Heratsi, Department of Physiology, Koryun 2, Yerevan, Armenia

Various genes level of activity in monolayer cultures of chondroprogenitor cells and chondrocytes isolated from articular cartilage of genetically modified mice was studied. Monolayer cultures of chondrocytes and chondroprogenitor cells were subjected to qPCR study to determine the target genes activity: col 2a1, Sox 9, Il 1a, Il 1b, CCL 2, CCL 3, CCL 4, CCL 5, MMP 3, MMP 13 and aggrecan (Aggr ). It was found that the activity of pro­inflammatory cytokines (Il1b, Il6, Il 8) is higher in chondroprogenitor cells, as well as the activity of metalloproteinases (MMP 3, MMP 13) responsible for the degradation of the matrix. Synthesis of type 2 collagen and agrecan in chondroprogenitor cells is higher than in chondrocytes, this pattern is observed in all strains of the studied animals. There is a high activity of type 2 collagen and aggrecan in both chondroprogenitor cells and chondrocytes of strain 6 isolated from the cartilage tissue, while the observed low activity of pro­inflammatory cytokines and metalloproteinases, which indicates a pronounced ability of this strain mice to repair damaged cartilage tissue.

Keywords: cytokines, cartilage, mice, osteoarthritis

TSitologiya i Genetika
2021, vol. 55, no. 4, 77-78

Current Issue
Cytology and Genetics
2021, vol. 55, no. 4, 396–403,
doi: 10.3103/S0095452721040125

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