TSitologiya i Genetika 2021, vol. 55, no. 4, 72-73
Cytology and Genetics 2021, vol. 55, no. 4, 379–387, doi: https://www.doi.org/10.3103/S0095452721040034

Studies on gamma rays induced cyto­morphological variations and procurement of some induced novel mutants in Kalmegh [Andrographis paniculata (Burm F.) Nees.]

Dwivedi K., Kumar K., Kumar G.

  1. Cytogenetics, Plant Breeding, Molecular Biology, and Biotechnology laboratory, University Department of Botany, Ranchi University, India­834001
  2. Naithani Plant Genetics Laboratory, Department of Botany, University of Allahabad, India­211001

In order to increase genetic variability for improvement and better adaptation in the present days’ changing climate, present investigation has been done in Andrographis paniculata (Burm. F.) Nees., (also known as Kalmegh in Ayurveda), an ethno­medicinal wild plant with highly immune­boosting properties. For the purpose, healthy, dry and homogeneous seeds were treated with four doses of gamma rays from Co­60 sources at the dose rate of 1.55 Gy per second(selected on the basis of LD50i.e. 25Gy (gray), 50Gy, 100Gy and 200Gy along with one set of non­irradiat­ed seeds and sown in triplicates in a completely randomized block design (CRBD). Higher doses were detrimental hence not suggested for further mutation breeding experiments. Ho­
wever, lower doses (viz. 25Gy and 50Gy) of gamma rays were stimulatory; had induced some significant (p > 0.5) variations in several lucrative traits of Andrographis paniculata (Kalmegh) viz. Plant height, leaf area, number of branches per plant, length of internodes (mm), fruit length, and seeds per fruit. Some induced novel mutants (viz. bushy, dark green leaves mutant and tricotyledonous leaf mutant in M2 generation) have also been procured. This is, to the best of our knowledge, first report on induction of tricotyledonous leaf mutant in A. paniculata using gamma rays.

Keywords: Andrographis paniculata (Kalmegh), Chromo­somal aberrations, Gamma rays, Genetic variation, Meiosis, Tricotyledon, Wild medicinal plants

TSitologiya i Genetika
2021, vol. 55, no. 4, 72-73

Current Issue
Cytology and Genetics
2021, vol. 55, no. 4, 379–387,
doi: 10.3103/S0095452721040034

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