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Effect of Cotreatment with static magnetic field and Cisdiamminedichloroplatinum (II) on apoptosis and cell cycle progression in HeLa cell line and Hu02
In this research, we evaluated the combined effect of static magnetic field and cisdiamminedichloroplatinum(II)(CDDP) on cell death, apoptosis and cell cycle in normal dermal fibroblast (Hu02) and HeLa cells. The cells were exposed to CDDP in the presence of 10 mT SMF for 24 and 48 hours. By using MTT assay the IC50 concentrations of CDDP were determined. The cytotoxic effects of this simultaneous application were studied using flowcytometric measurements for cell cycle and apoptosis. Based on the obtained results, the simultaneous application of CDDP and static magnetic field for 48 hours led to a significant increase in arrest of cell cycle progression of cells in G2/M phase, necrosis and late apoptosis in HeLa cells compared to the CDDPtreated group. In contrast, this combination led to a decrease in G2/M phase arrest, necrosis cells in Hu02 compared to drugtreated cells. In the presence of SMF, cancer cell susceptibility to CDDP was increased while it had some extent the protective effect on normal cells against cytotoxicity of the drug. Since there was an obvious difference in cell cycle stages and apoptosis between treated HeLa and Hu02 cells with combined SMF and CDDP, so this investigation proposes that cocompound of SMF and CDDP could be useful as way of reducing side effects of anticancer drug in normal cells and decreasing resistance of cancer cells to this drug.
Key words: apoptosis, cell cycle, CDDP, HeLa cell line, Hu02 cells, static magnetic field
E-mail: dr.hsoleimani arakmu.ac.ir
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