TSitologiya i Genetika 2021, vol. 55, no. 4, 67-69
Cytology and Genetics 2021, vol. 55, no. 4, 357–367, doi: https://www.doi.org/10.3103/S009545272104006X

Mahdian Expression Profile of MiR­200 Family Members and Their Targets in Prostate Cancer

Khorasani M., Shahbazi S., Abolhasani M., Shahrokh H., R.

  1. Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  2. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  3. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran
  4. Department of Pathology, Hasheminejad kidney Center, Iran University of Medical Sciences, Tehran, Iran
  5. Department of Uro­oncology, Hasheminejad kidney Center, Iran University of Medical Sciences, Tehran, Iran

Prostate cancer (PCa) shows the highest rate of new cancer cases in male population. Low sensitivity and specificity of traditional diagnostic tests have limited their implementation for early detection of PCa. The differential expression pattern of miR­200 family and their target genes has the potential of being considered as biomarkers for prostate cancer detection in combination with traditional screening. In this study we aimed to investigate changes in the expression profiles of the miR­200 family members/targets in PCa tissue samples. We examined the miR­200 family members and their target genes (TCF7L1, CTBP2, E2F3, CTNNB1, DLC1 and EP300) expression profile using quantitative Real­time PCR (samples n = 24). The results showed decreased mean expression level of miR­200a and miR­429 and DLC1 gene in tumor samples. Also, the expression level of E2F3, CTNNB1, EP300, CTBP2 and TCF7L1 genes was up­regulated in the tumor samples. ROC and AUC analysis showed that the combination of miR­200 family and their target genes expression profile successfully discriminated PCa samples from their non­tumor counterparts (miR­200 family AUC = 0.699, p < 0.01 and target genes AUC = 0.899, p < 0.0001, respectively). The results of this study indicate that the deregulated expression of the miR­200 family and their gene targets may have a role in the pathogenesis of PCa. We suggest further assessment of the expression profile of miR­200 family and their target genes in comparison with other PCa diagnostic biomarkers.

Keywords: Biomarker, MicroRNAs, miR­200 family, Prostate cancer, Quantitative Real­time PCR

TSitologiya i Genetika
2021, vol. 55, no. 4, 67-69

Current Issue
Cytology and Genetics
2021, vol. 55, no. 4, 357–367,
doi: 10.3103/S009545272104006X

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