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Phylogenetic analysis of the strain Bacillus subtilis IFBG MK-2 and production of riboflavin by its induced clones

Radchenko M.M., Tigunova .., Zelena L.B., Beiko N.Ye., Andriiash H.S., Shulga S.M.

 




SUMMARY. The aim of this work was to study the phylogenetic relationships of the new producer strain Bacillus subtilis IFBG -2 with existing strains and to increase the producers biosynthetic activity by mutagenesis. Se-quencing of the 16S rRNA of the IFBG MK-2 strain resulted in fragments of this gene with a total length of 1305 nucleotides. Phylogenetic analysis was performed and a dendrogram was constructed based on the nucleotide sequences of the 16S rRNA gene using the Neighbour joining method and the two-parameter Kimura model. It was shown that the similarity of sequenced fragments of 16S rRNA of strain IFBG MK-2 with fragments of deposited in GenBank sequence of this gene of a typical strain B. subtilis IAM 12118 was 99 %. According to the results of phylogenetic analysis, the IFBG strain MK-2 was identified as B. subtilis. Using the method of induced mutagenesis using UV radiation and sequential stepwise selection of clones, a mutant clone of B. subtilis IFBG MK-1A was obtained with a 50 % increase in the accumulation of riboflavin compared to the original strain.

Key words: Bacillus subtilis, riboflavin, mutagenesis, phylogeny, UV radiation, producer strain

Tsitologiya i Genetika 2021, vol. 55, no. 2, pp. 48-55

  1. SE Institute for Food Biotechnology and Genomics of National Academy of Sciences of Ukraine, 2a, Osypovskoho str., Kyiv, Ukraine, 04123
  2. Danylo Zabolotny Institute of Microbiology and Virology National Academy of Sciences of Ukraine, 154, Akademika Zabolotnogo str., Kyiv, Ukraine, 03143

E-mail: Shulga5 i.ua

Radchenko M.M., Tigunova .., Zelena L.B., Beiko N.Ye., Andriiash H.S., Shulga S.M. Phylogenetic analysis of the strain Bacillus subtilis IFBG MK-2 and production of riboflavin by its induced clones, Tsitol Genet., 2021, vol. 55, no. 2, pp. 48-55.

In "Cytology and Genetics":
M. M. Radchenko, O. O. Tigunova, L. B. Zelena, N. Ye. Beiko, H. S. Andriiash & S. M. Shulga Phylogenetic Analysis of the Bacillus subtilis IFBG MK-2 Strain and Riboflavin Production by Its Induced Clones, Cytol Genet., 2021, vol. 55, no. 2, pp. 145151
DOI: 10.3103/S0095452721020134


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