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Genetic differentiation of the round goby (Neogobius melanostomus) from certain localities of the Black and Azov Sea basin using microsatellite loci

Tarasjuk S.I., Zamorov V.V., Zaloilo O.V., Bielikova .Iu., Radionov D.B.

 




SUMMARY. Allelic variants were identified using SSR-markers and locus-specific features of the genetic structure of the round goby (Neogobius melanostomus) populations from five samples of the Black and Azov Sea basin were established. When comparing the values of observed Ho and expected H heterozygosity, it was found that the lowest average value for all analyzed loci was noted in the sample of Lake Yalpug (0.69 and 0.60, respectively), and the highest in the sample of Zmeyniy Island (0.89 and 0.83). The average polymorphic index content (PIC) ranged from 0.64 for Ame 129 locus to 0.68 for Ame 133, that indicates a high level of polymorphism of selected markers for this fish species. The phylogenetic analysis showed that the main cluster is formed by representatives of the round goby of the localities Zmeyniy Island and Dzharylgachsky bay (identity was 0.33), as well as the Dnieper-Bug estuary (0.26). The similarity of the round goby of lake Yalpug with representatives of the main cluster is 0.23. Pisces of this locality occupy a separate position on the dendrogram, which indicates the specificity of their genetic structure, that was formed historically under the influence of abiotic factors of the studied geographical zones during prolonged adaptation.

Key words: round goby Neogobius melanostomus, microsatellite loci, molecular genetic markers, genetic structure, alleles, localities

Tsitologiya i Genetika 2021, vol. 55, no. 3, pp. 35-47

  1. Odessa I.I. Mechnikov National University, Ukraine
  2. Institute of Fisheries, National Academy of Sciences of Ukraine, Kiev

E-mail: tarasjuk ukr.net, v.zamorov onu.edu.ua, ozaloilo yahoo.com, belikova.e.y gmail.com, pppankovae gmail.com

Tarasjuk S.I., Zamorov V.V., Zaloilo O.V., Bielikova .Iu., Radionov D.B. Genetic differentiation of the round goby (Neogobius melanostomus) from certain localities of the Black and Azov Sea basin using microsatellite loci, Tsitol Genet., 2021, vol. 55, no. 3, pp. 35-47.

In "Cytology and Genetics":
S. I. Tarasjuk, V. V. Zamorov, O. V. Zaloilo, O. Iu. Bielikova & D. B. Radionov Genetic Differentiation of the Round Goby (Neogobius melanostomus) from Certain Localities of the Black and Azov Sea Basin Using Microsatellite Loci, Cytol Genet., 2021, vol. 55, no. 3, pp. 236247
DOI: 10.3103/S0095452721030105


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