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Genetic identification and antimicrobial activity of Streptomyces sp. strain Je 16, isolated from rhizosphere soil of Juniperus excelsa Bieb.

Tistechok S.I., Tymchuk I.V., Korniychuk O.P., Fedorenko V.O., Luzhetskyy A.M., Gromyko O.M.


SUMMARY. Actinobacteria isolated from poorly explored biotopes are of interest as producers of biologically active compounds. Here, we report about the producer of lydicamycin Je 16, isolated from the rhizosphere soil of J. excelsa Bieb. collected from the Crimean Peninsula, Ukraine, that demonstrated its antagonistic activity against gram-positive bacteria. Based on the 16S rRNA gene sequence analysis, the strain Je 16 was affiliated to the Streptomyces genus. The crude extract from the biomass of this strain showed its activity against gram-positive bacteria, including polyresistant clinical isolates of Staphylococcus sp. The dereplication analysis of the extract of strain Je 16 resulted in determining lydicamycin and its congeners: 30-demethyllydicamycin, 30-demethyl-8-deoxylydicamycin and 8-deoxylydicamycin. MLSA analysis based on the 16S rRNA gene and five housekeeping genes atpD, gyrB, rpoB, recA, and trpB of the strain Je 16 showed a high level of homology with Streptomyces sp. ID38640.

Key words: Streptomyces, MLSA, phylogenetic analysis, lydicamycin, housekeeping genes

Tsitologiya i Genetika 2021, vol. 55, no. 1, pp. 33-41

  1. Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, Ukraine
  2. Department of Microbiology, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
  3. Department of Pharmaceutical Biotechnology, Saarland University, Saarbrucken, Germany
  4. Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrucken, Germany

E-mail: oleksandr.gromyko

Tistechok S.I., Tymchuk I.V., Korniychuk O.P., Fedorenko V.O., Luzhetskyy A.M., Gromyko O.M. Genetic identification and antimicrobial activity of Streptomyces sp. strain Je 16, isolated from rhizosphere soil of Juniperus excelsa Bieb., Tsitol Genet., 2021, vol. 55, no. 1, pp. 33-41.

In "Cytology and Genetics":
S. I. Tistechok, I. V. Tymchuk, O. P. Korniychuk, V. O. Fedorenko, A. M. Luzhetskyy & O. M. Gromyko Genetic Identification and Antimicrobial Activity of Streptomyces sp. Strain Je 16 Isolated from Rhizosphere Soil of Juniperus excelsa Bieb, Cytol Genet., 2021, vol. 55, no. 1, pp. 2835
DOI: 10.3103/S0095452721010138


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