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Study of a spring triticale collection concerning its resistance to leaf and stem rusts using allele-specific markers

Yerzhebayeva R.S., Bazylova T.A., Babissekova D.I., Amangeldiyeva A.A., Tajibayev D., Ydyrys A.

 


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SUMMARY. Development and use of rust-resistant varieties is the most environmentally-friendly and effective way to pro-tect wheat and triticale crops. To successfully select spring triticale for its rust resistance, it is necessary to have genetic material with effective genes. In order to identify carriers of leaf and stem rust resistance genes, a collection of spring triticale (86 samples) was studied using molecular markers and phytopathology methods. In artificial inoculation study, 81 % of samples in the spring triticale collection demonstrated high resistance (0R) to a stem rust population. 19 samples that showed resistance (05 %R) to the leaf rust population were selected. Identification of the collection using DNA markers made it possible to isolate samples with the Sr2 gene (19 samples), Sr22 (9 samples) and Lr28 (14 samples). No carriers of the Lr9 and Lr35/Sr39 genes were found in the collection. Samples with effective Sr2 and Sr22 genes were included into crosses to create stem rust resistant domestic varieties.

Key words: spring triticale, collection, variety, rust, resistance, gene, molecular marker

Tsitologiya i Genetika 2020, vol. 54, no. 6, pp. 54-64

  • LLP Kazakh Research Institute of Agriculture and Plant growing, 040909, Republic of Kazakhstan, Almaty Region, Almalybak v., Yerlepesov st., 1

E-mail: raushan_2008 mail.ru

Yerzhebayeva R.S., Bazylova T.A., Babissekova D.I., Amangeldiyeva A.A., Tajibayev D., Ydyrys A. Study of a spring triticale collection concerning its resistance to leaf and stem rusts using allele-specific markers, Tsitol Genet., 2020, vol. 54, no. 6, pp. 54-64.

In "Cytology and Genetics":
R. S. Yerzhebayeva, T. A. Bazylova, D. I. Babissekova, A. A. Amangeldiyeva, D. G. Tajibayev & A. Ydyrys Studying a Spring Triticale Collection for Resistance to Leaf and Stem Rusts using Allele-Specific Markers, Cytol Genet., 2020, vol. 54, no. 6, pp. 546554
DOI: 10.3103/S0095452720060043


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