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Calcium-dependent changes of cellular redox-homeostasis and heat resistance of wheat plantlets under influence of hemin carbon monooxide donor

Shkliarevskyi M.A., Karpets Yu.V., Kolupaev Yu.E., Lugovaya A.A., Dmitriev A.P.

 




SUMMARY. Carbon monooxide (CO) is considered as the important molecule-gasotransmitter involved in the regulation of functional activity of plants, including processes of adaptation to stress factors. However, the crosstalk of CO with other participants of signaling in plant cells remains low-studied. The role of various pools of calcium in the realization of hemin (carbon monooxide donor) influence on the generation and neutralization of reactive oxygen species (ROS) in cells of roots of wheat (Triticum aestivum L.) plantlets and their resistance to the damaging heating (45 C, 10 min) has been investigated by the inhibitory method. The treatment of plantlets with 5 M hemin caused the transitional increase in activity of exocellular peroxidase in roots and the intensifying of ROS generation with the maximum in 1,52 h after the influence starts. The chelator of exocellular calcium EGTA and the inhibitor of formation of inositol-1,4,5-phosphate neomycin, which reduces the influx of calcium into the cytosol from intracellular compartments, almost completely eliminated the in-crease in activity of exocellular peroxidase, caused by exogenous CO. At the same time, EGTA (completely) and neomycin (partially) leveled the increase in content of hydrogen peroxide in roots of plantlets, occurred under the influence of CO donor. The treatment of plantlets with hemin induced also the increase in activity of superoxide dismutase, catalase and intracellular peroxidase in roots. Both calcium antagonists removed these effects. And also in the presence of EGTA and neomycin, the positive influence of treatment with hemin on the state of biomembranes and survival of plantlets after the damaging heating was not shown. The conclusion is made that calcium, both exocellular and deposited in intracellular compartments, participates in the intensifying of ROS formation, induction of antioxidative system and development of heat resistance of wheat plantlets, affected by CO donor.

Key words: Triticum aestivum L., carbon monoxide, calcium, reactive oxygen species, heat resistance

Tsitologiya i Genetika 2020, vol. 54, no. 6, pp. 23-34

  1. Dokuchaev Kharkiv National Agrarian University, 62483, Kharkiv, Ukraine
  2. Karazin Kharkiv National University, 61022, Kharkiv, Ukraine
  3. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, 03143, Kyiv, Ukraine

E-mail: plant_biology ukr.net, dmitriev.ap gmail.com

Shkliarevskyi M.A., Karpets Yu.V., Kolupaev Yu.E., Lugovaya A.A., Dmitriev A.P. Calcium-dependent changes of cellular redox-homeostasis and heat resistance of wheat plantlets under influence of hemin carbon monooxide donor, Tsitol Genet., 2020, vol. 54, no. 6, pp. 23-34.

In "Cytology and Genetics":
M. A. Shkliarevskyi, Yu. V. Karpets, Yu. E. Kolupaev, A. A. Lugovaya & A. P. Dmitriev Calcium-Dependent Changes in Cellular Redox Homeostasis and Heat Resistance of Wheat Plantlets under Influence of Hemin (Carbon Monoxide Donor), Cytol Genet., 2020, vol. 54, no. 6, pp. 522530
DOI: 10.3103/S0095452720060109


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