TSitologiya i Genetika 2019, vol. 53, no. 3, 75-77
Cytology and Genetics 2019, vol. 53, no. 3, 250–260, doi: https://www.doi.org/10.3103/S0095452719030095

Isolation of antimicrobials from native plants of taif governorate

El-Shehawi A.M., Ahmed M.M., Elseehy M.M., Hassan M.M.

  1. Department of Biotechnology, Faculty of Science, Taif University, Taif, Saudi Arabia
  2. Department of Biology, Faculty of Science, Taif University, Taif, Saudi Arabia
  3. Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
  4. Department of Biochemistry, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt
  5. Department of Genetics, Faculty of Agriculture, Minufiya University, Sheben El-Kom, Egypt

Plant defensins are subgroup of plant antimicrobial peptides that have high potentials in developing safe natural agricultural and pharmaceutical biocontrol products as alternatives for the current chemical pesticides, insecticides, and antibiotics. In this study, the level of defensin gene expression was evaluated in floral buds of 9 native plants from Saudi Arabia environment using reverse transcription and polymerase chain reaction (RT-PCR). As defensins are highly expressed at the mRNA level in Ochradenus baccatus (Ob), it was used for the isolation of defensin peptides using ion exchange chromatography followed by gel filtration using Sephadex G50. SDS-PAGE of ion exchange purified proteins detected several bands in a range of 2–10 kD. These proteins were separated by gel filtration using Sephadex G50. The resultant peptide of 5kD showed an antimicrobial activity. The peptide inhibited Gram positive and Gram negative bacteria with IC50 ranged from 10 to 25 µg/mL. It also inhibited 25 to 60 % of fungal linear growth at concentrations of 12.5 and 25 µg/ mL. The present results demonstrate that, we could separate and purify a 5 kD peptide from Ob. This peptide could be utilized to develop natural antimicrobial products.

Keywords: Plant; antimicrobial peptides; defensins; gene expression; PCR; protein purification

TSitologiya i Genetika
2019, vol. 53, no. 3, 75-77

Current Issue
Cytology and Genetics
2019, vol. 53, no. 3, 250–260,
doi: 10.3103/S0095452719030095

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