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Description and characterization of Hellenic wheat germplasm for agronomical and seed quality parameters using phenotypical, biochemical and molecular approache

Xynias I.N., Mavromatis A.G., Korpetis E.G., Pankou C.I., Kozub N.O.

 




Hellenic wheat germplasm developed at the Cereal Institute of Thessaloniki, during the last fifty years, is depicted in the present study, using agronomical, biochemical and molecular criteria. The bread wheat germplasm is characterized by high yield potential, adaptation to the Mediterranean conditions, and advanced qualitative traits. The biochemical analysis revealed that three cultivars (Acheron, Elissavet and Orfeas) carry the 1BL.1RS wheat-rye translocation, which was further confirmed by molecular analysis. The cultivars Acheron and Elissavet, despite the presence of the translocation, have good bread making quality due to the presence of favorable genes controlling quality. Most of the Hellenic durum germplasm have high end product quality. The biochemical evaluation at the Gli-B1 locus revealed the presence of the alleles related with the γ-45 component, indicative of high technological traits. On the other side, it was found that two older durum wheat cultivars and a population tested, had the allele in* at the same locus, which is related with the γ-42 component, indicative of inferior pasta quality. Most of the Hellenic germplasm, either durum or bread wheat, carries very good agronomical and qualitative traits and could be used to broaden wheat biodiversity in S-E Mediterranean region.

Key words: Triticum spp., yield, quality, morphological, technological traits

Tsitologiya i Genetika 2019, vol. 53, no. 4, pp. 74-77

  1. Technological and Educational Institution of Western Macedonia, Department of Agricultural Technology, 53 100 Florina, Hellas
  2. Laboratory of Genetics and Plant Breeding, Aristotle University, 54124 Thessaloniki, Hellas
  3. Plant Breeding and Genetic Resources Institute, Hellenic Agricultural Organization- Demeter, 570 01 Thessaloniki, Hellas
  4. Institute of Plant Protection, Vasilkivska St., 33, Kyiv 03022, Ukraine
  5. Institute of Food Biotechnology and Genomics, NAS of Ukraine, Osypovskoho St., 2, Kyiv 04123, Ukraine

E-mail: ioannis_xynias hotmail.com, natalkozub gmail.com

Xynias I.N., Mavromatis A.G., Korpetis E.G., Pankou C.I., Kozub N.O. Description and characterization of Hellenic wheat germplasm for agronomical and seed quality parameters using phenotypical, biochemical and molecular approache, Tsitol Genet., 2019, vol. 53, no. 4, pp. 74-77.

In "Cytology and Genetics":
I. N. Xynias, A. G. Mavromatis, E. G. Korpetis, C. I. Pankou, N. O. Kozub Description and Characterization of Hellenic Wheat Germplasm for Agronomical and Seed Quality Parameters Using Phenotypical, Biochemical and Molecular Approaches, Cytol Genet., 2019, vol. 53, no. 4, pp. 337347
DOI: 10.3103/S0095452719040108


References

1. Papadakis, I.S., The Plant Breeding Institute 19231933, Sci. Bull. Inst. Plant Breed., 1933, vol. 15, pp. 136.

2. Papadakis, I.S., Statistical method for field experiments, Sci. Bull. Inst. Plant Breed., 1937, vol. 23, pp. 130.

3. Papadakis, I.S., Soil reaction and varietal adaptation, Sci. Bull. Inst. Plant Breed., 1935, vol. 18, pp. 116.

4. Papadakis, I.S., Varieties experiments in pots, Sci. Bull. Inst. Plant Breed., 1935, vol. 20, pp. 115.

5. Papadakis, I.S., The pocket method of varieties experiments, Sci. Bull. Inst. Plant Breed., 1935, vol. 21, pp. 17.

6. Kokolios, V., The cultivated wheat varieties in Hellas, Minist. Agric. Bull., 1959, vol. 3, pp. 178.

7. Talellis, D.E., The Increase in Agricultural Production and the Plant Breeding Institute, Thessaloniki: Ministry of Agriculture, Directorate of Agricultural Research. 1952.

8. Papadakis, I.S., The Greek agricultural and economic miracle (19221983). Lessons for foreigners and for us, Proc. Acad. Athens, 1983, vol. 58, 562580 (speech by Academician I. Papadakis).

9. Skorda E.A. The Breeding of bread wheat, in Suggestions on Cereals Meeting, February 1920, 1979, Thessaloniki, Greece, Thessaloniki: Ministry of AgricultureCereal Institute of Thessaloniki, 1979, pp. 817.

10. Anonymous, List of Cereal and Legume Grains of the State Seed Production, Athens: Ministry of Agriculture. 1978.

11. Anonymous, Cereal Varieties, Thessaloniki: Hellenic Ministry of Agriculture. 1985.

12. Anonymous, National Agricultural Research Foundation in Cereal Seed Production, Thessaloniki: NAGREFCereal Institute, 2011.

13. Korpetis, E., Hellenic Agricultural OrganizationDEMETER in Cereal Seed Production, Thessaloniki: HAO-DEMETER, 2016.

14. USDA, Crop Production in Greece and Italy, United States: Department of Agriculture, Foreign Agricultural Service, Commodity Intelligence Report, 2017. https://ipad.fas.usda.gov/highlights/2017/08/greeceitaly/ index.htm. Accessed November 11, 2017.

15. Kokolios, V. and Valtadoros, A., Factors, forms and criteria of wheat quality. Applications on breeding for quality research in Hellas, New Agricult. Rev., 1965, vol. 5, pp. 416.

16. Kokolios, V., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1963, Sci. Bull., 1965, vol. 4, p. 11.

17. Kokolios, V., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1964, Sci. Bull., 1966, vol. 11, p. 16.

18. Boudonas, G., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1965, Sci. Bull., 1966, vol. 14, p. 35.

19. Boudonas, G., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1967, Sci. Bull., 1968, vol. 24, pp. 36.

20. Boudonas, G., Valtadoros, A., Dimopoulos, J., and Pattakou, B., Qualité des blés Grecs de la recolte 1970, Sci. Bull., 1971, vol. 45, p. 36.

21. Boudonas, G., Valtadoros, A., and Pattakou, B., Qualité des blés Grecs de la recolte, Sci. Bull., 1973, vol. 50, p. 32.

22. Boudonas, G., Valtadoros, A., Pattakou, B., and Papastephanou, S., Qualité des blés Grecs de la recolte 1973, Sci. Bull., 1974, vol. 53, p. 32.

23. Boudonas, G., Valtadoros, A., Pattakou, B., and Papastephanou, S., Qualité des blés Grecs de la recolte 1973, Sci. Bull., 1974b, vol. 54, p. 32.

24. Boudonas, G., Pattakou, B., Papastephanou, S., Gioupsanis, T., Qualité des blés Grecs de la recolte 1974, Sci. Bull., 1976a, vol. 56, p. 36.

25. Boudonas, G., Pattakou, V., Papastephanou, S., and Gioupsanis, T., Qualité des blés Grecs de la recolte 1975, Sci. Bull., 1976b, vol. 58, p. 46.

26. Pattakou, V., Papastephanou, S., Gioupsanis, T., and Triantafillakos, N., Qualité des blés Grecs de la recolte 1978, Sci. Bull., 1979, vol. 66, p. 30.

27. Pattakou, V., Papastephanou, S., Triantafillakos, N., and Hadjisavva, S., Wheat quality, Crop 1985, Sci. Bull., 1986, vol. 85, p. 30.

28. Irakli, M. and Papageorgiou, M., Quality of bread and durum wheat crop 2000, NAGREFCereal Institute of Thessaloniki, Sci. Bull.New Ser., 2001, vol. 1, p. 30.

29. Papageorgiou, M. and Irakli, M., Quality of bread and durum wheat crops 2001 and 2002. NAGREFCereal Institute of Thessaloniki, Sci. Bull.New Ser., 2004, vol. 2, p. 44 (in Hellenic with English abstract).

30. Papageorgiou, M. and Irakli, M., Quality of bread and durum wheat crops 2003 and 2004, NAGREFCereal Institute of Thessaloniki, Sci. Bull.New Ser., 2005, vol. 3, p. 20.

31. Papageorgiou, M. and Irakli, M., Quality of bread and durum wheat crops 2005 and 2006, Sci. Bull.New Ser., 2007, vol. 4, p. 24.

32. Xynias, I.N., Kozub, N.O., and Sozinov, I.A., Seed storage protein composition of Hellenic bread wheat cultivars, Plant Breed., 2006, vol. 125, pp. 408410. https://doi.org/10.1111/j.1439-0523.2006.01242.x

33. Gouli-Vavdinoudi, E.K., Development of bread wheat varieties by selection in the absence of competition (T. aestivum L. Thell), Ph. D. Thesis, Thessaloniki: Scientific Yearbook of the Department of Agriculture Annex 8 of the 25th Volume, 1984.

34. Misic, T., Petrovic, S., and Mladenov, N., Characteristic of major Novi Sad winter wheat cultivars carrying wheatrye translocation, in Proc. Int. Symp. Breed. Small Grains, November 2427, 1998, Kragujevac, Yugoslavia, 1998, pp. 8595.

35. Howell, T., Hale, I., Jankuloski, L., Bonafede, M., Gilbert, M., and Dubcovsky, J., Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status, Theor. Appl. Genet., 2014, vol. 127, pp. 26952709. https://doi.org/10.1007/s00122-014-2408-6

36. Ehdale, B., Whitkus, R.W., and Waines, J.G., Root biomass, water-use efficiency, and performance of wheat rye translocations of chromosomes 1 and 2 in spring bread wheat Pavon, Crop Sci., 2003, vol. 43, pp. 710717. https://doi.org/10.2135/cropsci2003.7100

37. Carver, B.F. and Rayborn, A.L., Comparison of related wheat stocks possessing 1B or T1B1-Center-Dot-1Rs chromosome-grain and flour quality, Crop Sci., 1994, vol. 35, pp. 13161321. https://doi.org/10.2135/cropsci1994.0011183X003400060017x

38. Rabinovich, S.V., Importance of wheatrye translocations for breeding modern cultivars of Triticum aestivum L., Euphytica, 1998, vol. 100, pp. 323340. https://doi.org/10.1023/A:1018361819215

39. Payne, P.I., Holt, L.M., Jackson, E.A., and Law, C.N., Wheat storage proteins: their genetics and their potential for manipulation by plant breeding, Philos. Trans. R. Soc. London, 1984, vol. 304, pp. 359371.

40. Peros, Ch., Dalezios, G., Liakakos, E., Delis, K., and Xynias, I.N., Verification of the presence of the 1BL.1RS translocation in Hellenic bread wheat cultivars using the PCR method, in Book of Abstracts of the 14th Congress of the Hellenic Society of Genetics and Plant Breeding, Thessaloniki, October 1012, 2012, Thessaloniki, 2012, p. 70.

41. Mckendy, A.L., Taque, D.N., Finney, P.L., and Miskin, K.E., Effect of 1BL.1RS on milling and baking quality of soft red winter wheat, Crop Sci., 1996, vol. 36, pp. 848851. https://doi.org/10.2135/cropsci1996.0011183X003600040004x

42. Dimitrijevic, M., Petrovic, S., and Gustafson, J.P., The presence of wheat/rye translocation 1BL.1RS in wheat, in Proc. 2nd Balkan Symp. on Field Crops, June 1620, 1998, Novi Sad, Yugoslavia, Novi Sad, 1998, pp. 211213.

43. Karelov, A.V., Kozub, N.I., Sozinov, I.A., Sozinova, O., and Xynias, I.N., Molecular detection of the resistance to biotic stress conditions in Hellenic bread wheat commercial cultivars, in Proc. HAICTA 2017 Conf., September 2125, 2017, Chania, Crete, Hellas, 2017.

44. Kolmer, J.A., Singh, R.P., Garvin, D.F., Viccars, L., William, H.M., Huerta-Espino, J., Ogbonnaya, F.C., Raman, H., Orford, S., Bariana, H.S., and Lagudah, E.S., Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm, Crop Sci., 2008, vol. 48, pp. 18411852. https://doi.org/10.2135/cropsci2007.08.0474

45. Sissons, M., Role of durum wheat composition on the quality of pasta and bread, Food, 2008, vol. 2, pp. 7590.

46. Kudryavtsev, A.M., Development of the system of genetic markers of durum wheat (T. durum Desf.) and its use in scientific research and practice, Extended Abstract of Doctoral (Biol.) Dissertation, Moscow, Russia, 2007.

47. Xynias, I.N., Kozub, N., and Sozinov, I., Analysis of Hellenic durum wheat (Triticum turgidum L. var. durum) germplasm using gliadin and high-molecular-weight glutenin subunit loci, Cer. Res. Comm., 2011, vol. 39, pp. 415425. https://doi.org/10.1556/CRC.39.2011.3.11

48. Yupsanis, T., Identification and technological evaluation of wheat cultivars with electrophoresis of their gliadins, Agricult. Res., 1983, vol. 7, pp. 157167.

49. Damideaux, R., Autran, J.C., Grignak, P., and Feillet, P., Mise en evidence de relations applicables en selection entre l electrophoregramme des gliadines et les properties viscoelastiques du gluten de Triticum durum Desf, Comptes Rendes de lAcademie des Sci., 1978, vol. 287, pp. 701704.

50. Skerritt, J.H., Gluten proteins: genetics, structure and dough qualitya review, Ag. Biotech. News Inform., 1998, vol. 10, pp. 247N270N.

51. Carrillo, J.M., Martinez, M.C., Moita Brites, C., Nieto-Taladriz, M.Y., and Vasquez, J.F., Relationship between endosperm proteins and quality in durum wheat (Triticum turgidum L. var. durum), in Durum Wheat Improvement in the Mediterranean Countries, Royo, C., Nachit, M.M., Di Fonzo, N., and Araus, J.L., Eds., New Challenge Seminaires Mediterraneennes, 2000, vol. 40, pp. 463467.

52. Nevo, E., Korol, A.B., Beiles, A., and Fahima, T., Evolution of Wild Emmer and Wheat Improvement, Heidelberg: Springer-Verlag, 2002.

53. Nevo, E. and Payne, P.I., Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel. 1. Geographical patterns and ecological predictability, Theor. Appl. Genet., 1987, vol. 74, pp. 827836. https://doi.org/10.1007/BF00222968

54. Levy, A.A. and Feldman, M., Ecogeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat T. turgidum var. dicoccoides, Theor. Appl. Genet., 1988, vol. 75, pp. 651658. https://doi.org/10.1007/BF00289134

55. Zamani, J., Gouli-Vavdinoudi, E., and Roupakias, D.G., Anther culture of Greek cultivars and F1 crosses in bread wheat (Triticum aestivum), in Abstracts of 7th Conference of the Greek plant Breeding and Genetics Society, October 2123, Heraklio, Crete, Hellas, Heraclio, 1998.

56. Xynias, I.N., Zamani, I.A., Gouli-Vavdinoudi, E., and Roypakias, D.G., Effect of cold pre-treatment and incubation temperature on bread wheat (Triticum aestivum L.) anther culture, Cer. Res. Commun., 2001, vol. 29, pp. 331338.

57. Kozub, N., Xynias, I.N., Sozinov, I., Lisova, G., Zamani, I.A., Gouli-Vavdinoydi, E., and Roupakias, D.G., Screening of high-quality bread wheat dihaploid lines by the use of biochemical markers, Russ. J. Plant Physiol., 2006, vol. 53, pp. 396400. https://doi.org/10.1134/S1021443706030162

58. Lisova, G.M., Kozub, N.O., Sozinov, I.O., and Xynias, I.N., Donors of leaf rust, powdery mildew, and septoriosis resistance among Greek bread wheat cultivars and dihaploid lines, Plant Protec. Quaran., 2005, vol. 51, pp. 2229.

59. Payne, P.I. and Lawrence, G., Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat, Cer. Res. Commun., 1983, vol. 11, pp. 2934.

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