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Expression profiles of a tung tree phosphate transporter cdna and structural characteristics of the encoded protein
Plant phosphate transporters mediate phosphate acquisition, translocation, and recycling. A Vernicia fordii cDNA encoding a phosphate transporter was cloned using a reverse transcription polymerase chain reaction (PCR) and rapid amplification of cDNA ends. The resulting cDNA named Vernicia fordii Phosphate Transporter1;1 (VfPht1;1) encodes a predicted protein consisting of 533 amino acids. This protein, which was predicted to contain 12 transmembrane domains, is most similar (89,9 %) to a Ricinus communis predicted protein. The quantitative realtime PCR data revealed that VfPht1;1 is most highly expressed in mature leaf petioles. It is also highly expressed in seeds collected in June, when developing fruits require a large amount of phosphates. Our observations suggest that VfPht1;1 may have diverse functions related to phosphate uptake and translocation.
Key words: Vernicia fordii; phosphate transporter; VfPT1;1; gene cloning; gene expression
The Key Lab of Nonwood Forest Nurturing and Protection of the National Ministry of Education, Central South University of Forestry and Technology, Changsha 410004
E-mail: tanxiaofengcn 126.com, zhoujunqin1234 126.com; yuanjunchina 126.com; longhx886 126.com
1. Li, P., Zhang, X., Chen, Y., Lu, G., Zhou, G., and Wang, Y., Genetic diversity and germplasm resource research on tung tree (Vernicia fordii) cultivars, investigated by inter-simple sequence repeats, Afric. J. Biotech., vol. 7, no. 8, pp. 1054–1059.
2. He, G., Zhang, J., Hu, X., and Wu, J., Effect of aluminum toxicity and phosphorus deficiency on the growth and photosynthesis of oil tea (Camellia oleifera Abel.) seedlings in acidic red soils, Acta Physiol. Plant., 2010, vol. 33, no. 4, pp. 1285–1292. https://doi.org/10.1007/s11738-010-0659-7
3. Holford, I., Soil phosphorus: its measurement and its uptake by plants, Austral. J. Soil Res., 1997, vol. 35, pp. 227–239.
4. Hu, H.Q., Tan, C.Y., Tan, C., Cai, Ch., He, J., and Li, X., Availability and residual effects of phosphate rocks and inorganic P fractionation in a red soil of Central China, Nutr. Cycl. Agroecosyst., 2001, vol. 59, pp. 251–258.
5. Rae, A.L., Cybinski, D.H., Jarmey, J.M., and Smith, F.W., Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family, Plant Mol. Biol., 2003, vol. 53, nos. 1–2, pp. 27–36.
6. Ai, P., Sun, S., Zhao, J., Fan, X., Xin, W., Guo, Q., Yu, L., Shen, Q., Wu, P., Miller, A.J., and Xu, G., Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation, Plant J., 2009, vol. 57, no. 5, pp. 798–809. https://doi.org/10.1111/j.1365-313X.2008.03726.x
7. Shen, J.B., Yuan, L.X., Zhang, J.L., Li, H., Bai, Z., Chen, X., Zhang, W., and Zhang, F., Phosphorus dynamics: from soil to plant, Plant Physiol., 2011, vol. 156, no. 3, pp. 997–1005. https://doi.org/10.1104/pp.111.175232
8. Wu, Z.Y., Zhao, J.M., Gao, R.F., Hu, G., Gai, J., Xu, G., and Xing, H., Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max, PLoS One, 2011, vol. 6, no. 6, pp. 1–12. doi.org/ https://doi.org/10.1371/journal.pone.0019752
9. Shin, H., Shin, H.S., Dewbre, G.R., and Harrison, M.J., Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low-and high-phosphate environments, Plant J., 2004, vol. 39, no. 4, pp. 629–642. https://doi.org/10.1111/j.1365-313X.2004.02161.x
10. Misson, J., Thibaud, M.C., Bechtold, N., Raghothama, K., and Nussaume, L., Transcriptional regulation and functional properties of Arabidopsis Pht1;4, a high affinity transporter contributing greatly to phosphate uptake in phosphate deprived plants, Plant Mol. Biol., 2004, vol. 55, no. 5, pp. 727–741.
11. Yang, G.Z., Ding, G.D., Shi, L., and Xu, F., Characterization of phosphorus starvation-induced gene BnSPX3 in Brassica napus, Plant Soil, 2012, vol. 350, nos. 1–2, pp. 339–351. https://doi.org/10.1007/s11104-011-0913-9
12. Wu, P., Shou, H.X., Xu, G.H., and Lian, X., Improvement of phosphorus efficiency in riceon the basis of understanding phosphate signaling and homeostasis, Curr. Opin. Plant Biol., 2013, vol. 16, no. 2, pp. 205–212. https://doi.org/10.1016/j.pbi.2013.03.002
13. Shockey, J.M., Gidda, S.K., Chapital, D.C., Kuan, J.C., Dhanoa, P.K., Bland, J.M., Rothstein, S.J., Mullen, R.T., and Dyer, J.M., Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum, Plant Cell, 2006, vol. 18, no. 9, pp. 2294–2313. https://doi.org/10.1105/tpc.106.043695
14. Cao, H., Chapital, D.C., Shockey, J.M., and Klasson, K.T., Expression of tung tree diacylglycerol acyltransferase 1 in E. coli, BMC Biotechnol., 2011, vol. 11, pp. 1–13. https://doi.org/10.1186/1472-6750-11-73
15. Han, X., Lu, M., Chen, Y., Zhan, Z., Cui, Q., and Wang, Y., Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree during seed development, PLoS One, 2012, vol. 7, no. 8. https://doi.org/10.1371/journal.pone.0043084
16. Livak, K.J. and Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2(–Delta Delta C(T)) method, Methods, 2001, vol. 25, pp. 402–408.
17. Loth-Peereda, V., Orsini, E., Courty, P.E., Lota, F., Kohler, A., Diss, L., Blaudez, D., Chalot, M., Nehls, U., Bucher, M., and Martin, F., Structure and expression profile of the phosphate Pht1 transporter gene family in mycorrhizal Populus trichocarpa, Plant Physiol., 2013, vol. 156, no. 4, pp. 2141–2154. https://doi.org/10.1104/pp.111.180646
18. Pao, S.S., Paulsen, I.T., and Saier, M.H., Major facilitator superfamily, Microbiol. Mol. Biol. Rev., 1998, vol. 62, no. 1, pp. 1–34.
19. Seo, H.M., Jung, Y., Song, S., Kim, Y., Kwon, T., Kim, D.H., Jeung, S.J., Yi, Y.B., Yi, G., Nam, M.H., and Nam, J., Increased expression of OsPT1, a high-affinity phosphate transporter, enhances phosphate acquisition in rice, Biotechnol. Lett., 2008, vol. 30, no. 10, pp. 1833–1838. https://doi.org/10.1007/s10529-008-9757-7
20. Hinsinger, Ph., Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes: a review, Plant Soil, 2001, vol. 237, no. 2, pp. 173–195. https://doi.org/10.1023/A:1013351617532
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