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Molecular cloning and functional characterization of mouse innate immune sensor RIG

Tangjie Zhang, Saumendra N. Sarkar, Jianzhong Zhu

 




Innate immunity functions as the first line against infection, which is mediated by series of innate immune receptors. RIG-I like receptors (RLR) recognize the cytosolic nucleic acids mainly from viruses, trigger the type I interferon (IFN) production, and thus play essential roles in the anti-viral immunity. Here we cloned the mouse RIG-I (mRIG-I) cDNA into the pENTR vector using the TOPO cloning technology, and transferred the FLAG tagged mRIG-I gene from pENTR vector to destination pLenti viral vector. The expression of mRIG-I in transfected cells was detected by immunoblotting using anti-FLAG and anti-RIG-I antibodies. The transfected mRIG-I was active capable of inducing downstream IFN stimulated gene (ISG) gene transcription as well as protein expression.

Key words: Gateway cloning; ISG; mouse RIG-I; TOPO cloning

Tsitologiya i Genetika 2019, vol. 53, no. 4, pp. 70-71

  1. College Veterinary Medicine, Yangzhou University, Yangzhou 225009, P. R. China
  2. School of Medicine, University of Pittsburgh, Pittsburgh, USA, 15232

E-mail: slx yzu.edu.cn

Tangjie Zhang, Saumendra N. Sarkar, Jianzhong Zhu Molecular cloning and functional characterization of mouse innate immune sensor RIG, Tsitol Genet., 2019, vol. 53, no. 4, pp. 70-71.

In "Cytology and Genetics":
Tangjie Zhang, Saumendra N. Sarkar, Jianzhong Zhu Molecular Cloning and Functional Characterization of Mouse Innate Immune Sensor RIG-I, Cytol Genet., 2019, vol. 53, no. 4, pp. 325329
DOI: 10.3103/S0095452719040121


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