TSitologiya i Genetika 2019, vol. 53, no. 4, 50-59
Cytology and Genetics 2019, vol. 53, no. 4, 307–314, doi: https://www.doi.org/10.3103/S0095452719040042

Cell selection of sugar beet in vitro for resistance to cultural filtrate of Fusarium оxysporum

Yerzhebayeva R.S., Abekova A.M., Bersimbaeva G.H., Konysbekov K.T., Bastaubaeva S.O., Roik N.V., Urazaliev K.R.

SUMMARY. Stepwise selection in vitro was conducted on well-proliferating callus tissue of sugar beet, capable of morphogenesis. The 20-day-old cultural filtrate of Fusarium оxysporum was used as a selective agent. Common specificities of the behavior of sugar beet callus cells, cultivated in stress conditions with increasing concentrations of the cultural filtrate were determined – 5, 10, 15 and 20 %. The research revealed that low concentration of the cultural filtrate (CF) of the pathogen (5 %) stimulated callusogenesis process considerably, whereas high concentrations (10–20 %) had an inhibiting effect. Cell and tissue selection allowed selecting the calluses, which preserved their capability of normal growth in the presence of sublethal concentrations of the selective agent. The method of stepwise selection was used to select 82 callus lines, characterized by their resistance to the toxic impact of CF. The isolated callus lines were used to obtain 47 regenerant plants, adapted to soil conditions. Only 24 plants passed the testing for resistance to Fusarium оxysporum in vivo.

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TSitologiya i Genetika
2019, vol. 53, no. 4, 50-59

Current Issue
Cytology and Genetics
2019, vol. 53, no. 4, 307–314,
doi: 10.3103/S0095452719040042

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References

1. McGrath, J.M., Townsend, B.J., Sugar Beet, Energy Beet, and Industrial Beet. Industrial Crops. Handbook of Plant Breeding, New York: Springer, 2015, vol. 9. https://doi.org/10.1007/978-1-4939-1447-0_5

2. Maui, A., Urazaliev, K., and Abekova, A., Diseases of sugar beet in Kazakhstan, in Agricultural Research Updates, New York: Nova Sci. Publ., 2016, vol. 12, no. 9, pp. 143–171.

3. Podvigina, O.A., Theoretical substantiation and approaches in using biotechnological methods in sugar beat breeding, Doctoral (Agricult.) Dissertation, Voronezh: Voronezh. Agrar. Univ., 2003. OD, 71:04-6/37-2

4. Soboleva, G.V., Influence of osmotic stress on processes of growth and morphogenesis in long-term callus cultures of pea (Pisum sativum L.), Sci. Prod. J. “Grain Legumes and Cereal Crops”, 2013, no. 1(5), pp. 8–15.

5. Rao, S. and Sandhya, H., In Vitro Selection of Disease-Resistant Plants. Plant Tissue Culture: Propagation, Conservation and Crop Improvement, Singapore: Springer, 2016. https://doi.org/10.1007/978-981-10-1917-3_17

6. Binarova, P., Nedelnik, J., Fellner, M., and Nedbalkova, B., Selection for resistance to filtrates of Fusarium spp. in embryogenic cell suspension culture of Medicago sativa L., Plant Cell Tiss. Organ Cult., 1990, vol. 22, no. 3, pp. 191–196. https://doi.org/10.1007/BF00033635

7. Chawla, H.S. and Wenzel, G., In vitro selection for fusaric acid resistant barley plants, Plant Breed., 1987, vol. 99, pp. 159–163. https://doi.org/10.1111/j.1439-0523.1987.tb01166.x

8. Hashem, E.A., Abdalla, H.E., Hussein, Y.A., and Abd-Elnabi, M.A., In vitro selection of soybean callus resistant to Fusarium oxysporum metabolites, in Proceedings Third Environment Conference, Faculty of Sci., Zagazig Univ., 2008, pp. 1–19.

9. Kasem, Z.A., Mesterházy, B., Bartyk, T., and Sági, F., In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. II. Culture filtrate technique and inheritance of Fusarium-resistance in the somaclones, Euphytica, 1996, vol. 91, no. 3, pp. 341–349. https://doi.org/10.1007/BF00033096

10. Khan, I.A., Alam, S.S., and Jabbar, A., Purification of phytotoxin from culture filtrates of Fusarium oxysporum f. sp. ciceris and its biological effects on chickpea, Pak. J. Bot., 2004, vol. 36, pp. 871–880.

11. Rutherford, R., Stuart, Snyman., Sandy, J., and Watt, M.P., In vitro generation of somaclonal variant plants of sugarcane for tolerance to Fusarium sacchari, Plant Cell Rep., 2013, vol. 32, no. 2, pp. 249–262. https://doi.org/10.1007/s00299-012-1359-0

12. Purwati, R.D. and Harran, S., Sudarsono. In vitro selection of abaca for resistance to Fusarium oxysporum f. sp. cubense, Hayati J. Biosci., 2007, vol. 14, no. 2, pp. 65–70. https://doi.org/10.4308/hjb.14.2.65

13. Esmaiell, N.M., Al-Doss, A.A., and Barakat, M.N., In vitro selection for resistance to Fusarium oxysporum f. sp. dianthi and detection of genetic polymorphism via RAPD analysis in carnation, Med. Plants Res., vol. 6, no. 23, pp. 3997–4004. https://doi.org/10.5897/JMPR12.150

14. Chen, W. and Swart, W.J., The in vitro phytotoxicity of culture filtrates of Fusarium oxysporum to five genotypes of Amaranthus hybridus, Euphytica, 2002, vol. 127, pp. 61–67.

15. Urazaliev, K., Abekova, A., Bazylova, T., Bersimbaeva, G., Daniyarova, A., and Massonichich-Shotunova, R., Somaclonal variation of sugar beet resistant to pathogenic root rot Fusarium oxysporum var. orthoceras, Genetika, 2013, vol. 45, pp. 629–640. https://doi.org/10.2298/gensr1303629u

16. Kalashnikova, E.A., Cell breeding of plants for resistance to fungal diseases, Doctoral (Biol.) Dissertation, Moscow: Timiryazev Moscow Agricultural Academy, 2003. OD, 71:04-3/116.

17. Murashige, T. and Skoog, F., A revised medium for rapid growth and bio assays with tobacco culture, Physiol. Plantarum, 1962, vol. 15, pp. 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

18. Xu, Q.L., Xie, Y.H., Ru, H., Hu, X., Wang, Ch.Y., and Wang, X.Yu., Efficient plant regeneration in vitro from red leaf beet via organogenesis, Russ. J. Plant Physiol., 2009, vol. 56, no. 4, pp. 546–550. https://doi.org/10.1134/S1021443709040153

19. Walker, J.C., Plant Pathology, New York: McGraw-Hill Book, 1969, 3rd ed.

20. Freytag, A.H., Anan, S.C., Rao-Ardelli, A.P., and Owens, L.D., An improved medium for adventitious shoot formation and callus induction in Beta vulgaris L. in vitro, Plant Cell Rep., 1988, vol. 7, pp. 30–34. https://doi.org/10.1007/BF00272972

21. Golovko, A.E., Dovzhenko, A.A., and Gleba, Yu.Yu., Genetic transformation of sugar beet: the evolution of attitudes and methodological approaches, Cytol. Genet., 2005, vol. 39, no. 3, pp. 30–36.

22. Hisano, H., Kimoto, Y., Hayakawa, H., Takeichi, J., Domae, T., Hashimoto, R., Abe, J., Asano, S., Kanazawa, A., and Shimamoto, Y., High frequency Agrobacterium mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritime, Plant Cell Rep., 2004, vol. 22, pp. 910–918. https://doi.org/10.1007/s00299-004-0773-3

23. Dovzhenko, A. and Koop, H., Sugar beet (Beta vulgaris L.): shoot regeneration from callus and callus protoplasts, Planta, 2003, vol. 217, pp. 374–381. https://doi.org/10.1007/s00425-003-1006

24. Mishutkina, Ya.V. and Gaponenko, A.K., Sugar beet (Beta vulgaris L.) morphogenesis in vitro: effects of phytohormone type and concentration in the culture medium, type of explants, and plant genotype on shoot regeneration frequency, Russ. J. Genet., 2006, vol. 42, no. 2, pp. 150–157. https://doi.org/10.1134/S1022795406020086