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Cell selection of sugar beet in vitro for resistance to cultural filtrate of Fusarium îxysporum
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SUMMARY. Stepwise selection in vitro was conducted on well-proliferating callus tissue of sugar beet, capable of morphogenesis. The 20-day-old cultural filtrate of Fusarium îxysporum was used as a selective agent. Common specificities of the behavior of sugar beet callus cells, cultivated in stress conditions with increasing concentrations of the cultural filtrate were determined – 5, 10, 15 and 20 %. The research revealed that low concentration of the cultural filtrate (CF) of the pathogen (5 %) stimulated callusogenesis process considerably, whereas high concentrations (10–20 %) had an inhibiting effect. Cell and tissue selection allowed selecting the calluses, which preserved their capability of normal growth in the presence of sublethal concentrations of the selective agent. The method of stepwise selection was used to select 82 callus lines, characterized by their resistance to the toxic impact of CF. The isolated callus lines were used to obtain 47 regenerant plants, adapted to soil conditions. Only 24 plants passed the testing for resistance to Fusarium îxysporum in vivo.
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