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Investigation of allilic diversity of HMW glutenins genes of wheat varieties and lines used in the breeding process in the republic of Belarus by PCR markers
SUMMARY. The allelic composition of genes encoding glutenins was studied in the collection of 236 winter wheat samples and 98 spring wheat samples of Belarusian and foreign breeding. 13 alleles among winter wheat samples and 11 alleles among spring wheat samples were revealed. This is generally less than in European, Asian and African countries. 36 different genotypes were identified among studied samples. Varieties and lines with genotypes Glu-A1c, Glu-B1c, Glu-D1d (16,2 % of the total number of studied samples), Glu-A1a, Glu-B1c, Glu-D1d (13,7%), Glu-A1b, Glu-B1c, Glu-D1d (12,7 %) are the most frequent among winter wheat samples. Varieties and lines with genotypes Glu-A1a, Glu-B1c, Glu-D1d (22,5 %), Glu-A1a, Glu-B1c, Glu-D1a (16,4 %), Glu-A1c, Glu-B1f, Glu-D1d (13,3 %) prevail among spring wheat samples. The allele of the gene encoding the primary structure of the Bx-subunit with a maximum identity degree 99 % relatively to the Bx-subunits represented in the GenBank database was revealed in the spring wheat line KP-406/11 genome. It contains an open reading frame with a length of 2367 bp and has the highest identity degree with the nucleotide sequence of the Bx14-subunit. The 789 amino acid sequence encoded by this allele differs from Bx14 by replacement of amino acid residues in three positions – 662, 780 and 788. It was designated as Bx14.1 (accession number in Genbank MH108092).
E-mail: E.Fomina igc.by, S.Malyshev igc.by, xaccka gmail.com, cytoplasmic mail.ru, O.Urbanovich igc.by
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