SUMMARY. At the present time, the uncontrolled interbreeding of bees that belong to different subspecies or races of Apis mellifera has become a threat in Ukraine. Conserving the gene pool of local bee breeds, which are well adapted to the local environmental conditions, is an urgent problem that cannot be solved without the application of molecular methods for monitoring of the genetic composition of local populations. To clarify the origin of the Carpathian bee and to develop molecular methods for its identification, sequencing and comparative analysis of the CoI-CoII region of the mitochondrial genome of the Carpathian bee and representatives of several subspecies of A. mellifera was performed. In the studied region, a mutation specific for the Carpathian bee was discovered. It was found that Carpathian bee originates from the Central European subspecies A. m. carnica and represents the ecotype that arose from the migration of this subspecies to the East, which resulted in the formation of Carpathian populations of honey bees.
Keywords: biodiversity, molecular evolution and phylogeny, mitochondrial DNA, cytochrome oxidase, Apis mellifera
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1. Van der Zee, R., Pisa, L., Andonov, S., Brodschneider, R., Charrière, J.D., Chlebo, R., Coffey, M.F., Crailsheim, K., Dahle, B., Gajda, A., Gray, A., Drazic, M.M., Higes, M., Kauko, L., Kence, A., Kence, M., Kezic, N., Kiprijanovska, H., Kralj, J., Kristiansen, P., Hernandez, R.M., Mutinelli, F., Nguyen, B.K., Otten, C., Özkırım, A., Perna, S.F., Peterson, M., Ramsay, G., Santrac, V., Soroker, V., Topolska, G., Uzunov, A., Vejsnæs, F., Wei, S., and Wilkins, S., Managed honey bee colony losses in Canada, China, Europe, Israel and Turkey, for the winters of 2008–9 and 2009–10, J. Apicult. Res., 2009, vol. 51, no. 1, pp. 100–114. https://doi.org/10.3896/IBRA.1.51.1.12
2. Brodschneider, R., Gray, A., Van der Zee, R., Adjlane, N., Brusbardis, V., Charriere, J.D., Chlebo, R., Coffey, M.F., Crailsheim, K., Dahle, B., Danihlík, J., Danneels, E., De Graaf, D.C., Dražić, M.M., Fedoriak, M., Forsythe, I., Golubovski, M., Gregorc, A., Grzęda, U., Hubbuck, I., Tunca, R.I., Kauko, L., Kilpinen, O., Kretavicius, J., Kristiansen, P., Martikkala, M., Martín-Hernández, R., Mutinelli, F., Peterson, M., Otten, C., Ozkirim, A., Raudmets, A., Simon-Delso, N., Soroker, V., Topolska, G., Vallon, J., Vejsnæs, F., and Woehl, S., Preliminary analysis of loss rates of honey bee colonies during winter 2015/16 from the COLOSS survey, J. Apicult. Res., 2016, vol. 55, no. 5, pp. 375–378. https://doi.org/10.1080/00218839.2016.1260240
3. Fedoriak, M.M., Timochko, L.I., Kulmanov, O.M., Volkov, R.A., and Rudenko, S.S., Winter losses of honey bee (Apis mellifera L.) colonies in Ukraine (monitoring results of 2015–2016), Ukr. J. Ecol., 2017, vol. 7, no. 4, pp. 604–613. https://doi.org/10.15421/2017_167
4. Ruttner, F., Biogeography and Taxonomy of Honeybees, Berlin: Springer-Verlag, 1988. https://doi.org/10.1007/978-3-642-72649-1
5. Savushkina, L.N., Races of bees regionalized in Russia, Sci. Herald Nat. Agrar. Univ., 2006, vol. 94, pp. 113–117.
6. Bagriy, I.G., About relatives of Ukrainian bees, Sci. Herald Nat. Agrar. Univ., 2006, vol. 94, pp. 90–93.
7. Cherevatov, V.F., Ferkaljak, V.Y., and Volkov, R.A., Uncontrolled hybridization of honeybees (Apis mellifera L.) in the territory of Ivano-Frankivsk region, Bull. Vavilov Soc. Genet. Breed. Ukraine, 2014, vol. 12, no. 2, pp. 234–240.
8. Cherevatov, V.F., Ferkaljak, V.Y., and Volkov, R.A., Hybridization of honey bees (Apis mellifera L.) in the territory of Chernivtsy region (Ukraine), National Museum of Ethnography and Natural History of Moldova Sci. Bull., 2016, vol. 24, no. 37, pp. 62–67.
9. Metlitska, O.I., Polishchuk, V.P., and Taran, S.I., The use of comparative and molecular-genetic evaluation under study of strain genuineness of Ukrainian bees, Anim. Biol., 2010, vol. 12, no. 1, pp. 254–259.
10. Meixner, M., Pinto, M., and Bouga, M., Standard methods for characterising subspecies and ecotypes of Apis mellifera, J. Apic. Res., 2013, vol. 52, no. 4, pp. 1–28. https://doi.org/10.3896/IBRA.1.52.4.05
11. Achou, M., Loucif-Ayad, W., Legout, H., Hmidan, H., Alburaki, M., and Garnery, L., An insightful molecular analysis reveals foreign honeybees among Algerian honeybee populations (Apis mellifera L.), J. Data Mining Genom. Proteom., 2015, vol. 6, no. 1. https://doi.org/10.4172/2153-0602.1000166
12. Pentek-Zakar, E., Oleksa, A., Borowik, T., and Kusza, S., Population structure of honey bees in the Carpathian Basin (Hungary) confirms introgression from surrounding subspecies, Ecol. Evol., 2015, vol. 5, no. 23, pp. 5456–5467.
13. Polishchuk, V.P. and Gaidar, V.A., Apiary, Kiev: Perfect Style, 2008.
14. Franck, P., Garnery, L., and Loiseau, B.P., Genetic diversity of the Honey bee in Africa: microsatellite and mitochondrial data, Heredity, 2001, vol. 86, pp. 420–430. https://doi.org/10.1046/j.1365-2540.2001.00842.x
15. Martimianakis, M., Klossa-Kilia, E., Bouga, M., and Kilias, G., Phylogenetic relationships of Greek Apis mellifera subspecies based on sequencing of mtDNA segments (COI and ND5), J. Apicult. Res., 2011, vol. 50, no. 1, pp. 42–50. https://doi.org/10.3896/IBRA.1.50.1.05
16. Whitfield, C.W., Behura, S.K., Berlocher, S.H., Clark, A.G., Johnston, J.S., Sheppard, W.S., Smith, D.R., Suarez, A.V., Weaver, D., and Tsutsui, N.D., Thrice out of Africa: ancient and recent expansions of the honey bee, Apis mellifera, Science, 2006, vol. 314, pp. 642–645. https://doi.org/10.1126/science.1132772
17. Wallberg, A., Han, F., Wellhagen, G., and Dahle, B., A worldwide survey of genome sequence variation provides insight into the evolutionary history of the honeybee Apis mellifera, Nat. Genet., 2014, vol. 46, pp. 1081–1088. https://doi.org/10.1038/ng.3077
18. Alburaki, M., Bertrand, B., Legout, H., Moulin, S., Alburaki, A., Sheppard, W.S., and Garnery, L., A fifth major genetic group among honeybees revealed in Syria, BMC Genet., 2013, vol. 14, pp. 117–128. https://doi.org/10.1186/1471-2156-14-117
19. Rortais, A., Arnold, G., Alburaki, M., Legout, H., and Garnery, L., Review of the DraI COI-COII test for the conservation of the black honeybee (Apis mellifera mellifera), Cons. Genet. Res., 2011, vol. 3, pp. 383–391. https://doi.org/10.1007/s12686-010-9351-x
20. Ostroverkhova, N.V., Konusova, O.L., Kucher, A.N., Kireeva, T.N., Vorotov, A.A., and Belikh, E.A., Genetic diversity of the locus COI-COII of mitochondrial DNA in honeybee populations (Apis mellifera L.) from the Tomsk region, Russ. J. Genet., 2015, vol. 51, pp. 80–90. https://doi.org/10.1134/S102279541501010X
21. Garnery, L., Cornuet, J., and Solignac, M., Evolutionary history of the honey bee Apis mellifera inferred from mitochondrial DNA analysis, Mol. Ecol., 1992, vol. 1, pp. 145–54. https://doi.org/10.1111/j.1365-294X.1992
22. Rogers, S.O. and Bendich, A.J., Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues, Plant Mol. Biol., 1985, vol. 5, no. 2, pp. 69–76. https://doi.org/10.1007/BF00020088
23. Crozier, R.H. and Crozier, Y.C., The mitochondrial genome of the honeybee Apis mellifera: complete sequence and genome organization, Genetics, 1993, vol. 133, no. 1, pp. 97–117.
24. Thompson, J.D., Higgins, D.G., and Gibson, T.J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res., 1994, vol. 22, no. 22, pp. 4673–80. https://doi.org/10.1093/nar/22.22.4673
25. Kerek, S.S. and Kerek, P.M., Peculiarities of breed characteristics of native bees from lowland areas of Transcarpathia, Beekeeping Ukraine, 2017, vol. 2, pp. 115–128.
26. Cornuet, J., Garnery, L., and Solignac, M., Putative origin and function of the intergenic region between COI and COII of Apis mellifera mitochondrial DNA, Genetics, 1991, vol. 128, no. 2, pp. 393–403.
27. Franck, P., Koeniger, N., Lahner, G., and Crewe, R., Evolution of extreme polyandry: an estimate of mating frequency in two African honeybee subspecies, Apis mellifera monticola and A.m. scutellata, Insects Soc., 2000, vol. 47, no. 4, pp. 464–470. https://doi.org/10.1007/PL00001732
28. Delaney, D.A., Meixner, M.D., Schiff, N.M., and Sheppard, W.S., Genetic characterization of commercial honey bee (Hymenoptera: Apidae) populations in the United States by using mitochondrial and microsatellite markers, Ann. Entomol. Soc. Am., 2009, vol. 102, no. 4, pp. 666–673. https://doi.org/10.1603/008.102.0411