Effects of exogenous cytokinins on spore germination and gametophyte morphogenesis of Dryopteris filix­mas (L.) Schott in vitro culture

Romanenko K.O., Kosakivska I.V., Babenko L.M., Vasheka O.V., Romanenko P.O., Negretsky V.A., Minarchenko V.M.

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SUMMARY. The effects of exogenous cytokinin phytohormones: kinetin, zeatin, 6-benzylaminopurine, N⁶-2-isopenteny-ladenine on the pattern of spore germination as well as gametophyte morphology and growth features of Dryopteris filix-mas (L.) Schott in vitro culture have been studied. It was established that at the concentration of 10^–5 М all studied cytokinins significantly retarded spore germination, inhibited gametophyte growth, caused deformations and changes in the thallus size, suppressed the development of reproductive structures and sporophyte growth. The reduction of the hormone concentration to 10^–8 М stimulated the gametophyte development, induced cell divisions, particularly in the apical zone, due to which some of thalli were deformed, promoted the production of rhizoids, affected the formation of antheridia and archegonia and slowed the sporophyte development.

Key words: Dryopteris filix-mas, gametophyte, spores, prothallium, thallus, cytokinins, kinetin, zeatin, 6-benzylaminopurine, ​N⁶-isopentenyladenine

Tsitologiya i Genetika 2019, vol. 53, no. 3, pp. 12-24

E-mail: katerynaromanenko4 gmail.com, irynakosakivska gmail.com, lilia.babenko gmail.com, olena.vasheka gmail.com, petrorom ukr.net, negretsky ukr.net, valminar ukr.net

Romanenko K.O., Kosakivska I.V., Babenko L.M., Vasheka O.V., Romanenko P.O., Negretsky V.A., Minarchenko V.M. Effects of exogenous cytokinins on spore germination and gametophyte morphogenesis of Dryopteris filix­mas (L.) Schott in vitro culture, Tsitol Genet., 2019, vol. 53, no. 3, pp. 12-24.

In "Cytology and Genetics":
K. O. Romanenko, I. V. Kosakivska, L. M. Babenko, O. V. Vasheka, P. O. Romanenko, V. A. Negretsky, V. M. Minarchenko Effects of Exogenous Cytokinins on Spore Germination and Gametophyte Morphogenesis of Dryopteris filix-mas (L.) Schott in vitro Culture, Cytol Genet., 2019, vol. 53, no. 3, pp. 192–201
DOI: 10.3103/S0095452719030034

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