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Using micronucleus test in Pinus pallasiana D. Don and Picea abies (L.) Kàrst. to assess the influence of technogenic pollution
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SUMMARY. The results of the study of micronuclei (MN) frequency in the meristem of seedlings Pinus pallasiana D. Don and Picea abies (L.) Karst. from natural populations and plantings of technogenic contaminated territories are presented. The level of cells with MN was 0.95 ± 0.13 % in the control population of P. pallasiana. An increase in the level of cells with MN was observed in the conditions of introduction and technogenic pollution (2,41–3,38 %). Similar results are shown for P. abies. The pathological cell contained a different amount of MN (from 1 to 2 for P. pallasiana and from 1 to 6 for P. abies). The size of the MN have varied from 0,13 to 14,93 % diameters of the main nuclei in P. pallasiana cells and from 0,07 to 61,87 % in P. abies. Multinuclear cells and cells with an irregular shape nuclear were observed. Correlation dependence was established between cytogenetic disorders and MN frequency (r = 0,98). It is proposed to use the MN of seedlings of studied species to assess the genotoxicity of technogenically contaminated areas.
E-mail: ivivkor gmail.com, belonojkoua gmail.com, botgard ukpost.ua
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