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Enigmatic phenomenon of secondary association among bivalents in Guar (Cyamopsis tetragonoloba (L.) Taub.)
Cytogenetical assessment of pollen mother cells of Guar (Cyamopsis tetragonoloba (L.) Taub.) in response to treatment of chemical mutagen was done. Ethyl Methane Sulphonate (EMS) revealed possession of an enigmatic behaviour among chromosomal bivalents i.e. formation of secondary association. Chromosomes exhibited a unique tendency of getting associated in close proximity and form discrete pairs. The phenomenon of secondary chromosomal association has been ascribed to the existence of similar cohesive homology or malfunctioning in chiasma formation among the associating bivalents. The association of bivalents was witnessed to be executed in either end to end fashion or sideways association. Various other chromosomal abnormalities were found, but secondary association was the preponderant abnormality which exhibited a continuous increase in its magnitude with increase in EMS concentration. Alongwith secondary associations, stickiness of chromosomes at anaphase was observed in considerable frequency. A significant influence of chromosomal association on the post meiotic product was also experienced, which in turn, led to influence the meiotic index. Abnormal sporads in the form of triads, dyads, monads and polyads, alongwith normal tetrad were seen. Pollen fertility percent was seen to decline from control to the highest concentration of EMS due to anomalies of preceding stages.
Key words: Guar, pollen mother cells (pmc), secondary association, Meiotic Index (MI), pollen fertility
1. Kuwada, Y., A cytological study of Oryza sativa L., Bot. Mag., 1910, vol. 24, pp. 267–280.
2. Ishikawa, M., Cytologische Studien and von Dahlien, Bot. Mag., 1911, vol. 25, pp. 1–8.
3. Marchal, E., Recherches cytologiques sur le genre Amblystegium, Bull. Soc. R. Bot. Belg., 1912, vol. 51, pp. 189–200.
4. Darlington, C.D. and Moffett, A.A., Primary and secondary chromosome balance in Pyrus, J. Genet., 1930, vol. 22, pp. 129–151.
5. Darlington, C.D., Cytology, London: Churchill, 1965.
6. Jelenkovic, G., Shifriss, Q., and Harington, E., Association and distribution of meiotic chromosomes in a haploid of Ricinus communis L., Cytologia, 1980, vol. 45, pp. 571–577.
7. Lawrence, W.J.C., The secondary association of chromosomes, Cytologia, 1931, vol. 2, pp. 352–384.
8. Malgwi, M.M., Oyewole, S.O., and Khan, A.U., Chromosomes and secondary associations in tetraploid Cleome polyanthera L., Nucleus, 1997, vol. 40, pp. 20–25.
9. Kumar, G. and Chaudhary, N., Secondary chromosomal association in kidney bean (Phaseolus vulgaris L.), Jor. Jour. Bio. Sci., 2014, vol. 7, no. 1, pp. 71–74.
10. Kumar, D. and Singh, N., Guar in India, Scientific Publishers, 2002.
11. Mukhtar, H.M., Ansari, S.H., Ali, M., Bhat, Z.A., and Naved, T., Effect of aqueous extract of Cyamopsis tetragonoloba Linn. beans on blood glucose level in normal and alloxan-induced diabetic rats, Ind. J. Exp. Biol., 2004, vol. 42, pp. 1212–1215.
12. Shyale, S., Chowdary, K., Krishnaiah, Y., and Bhat, N.K., Pharmacokinetic evaluation and studies on the clinical efficacy of guar gum-based oral drug delivery systems of albendazole and albendazole-β-cyclodextrin for colon targeting in human volunteers, Drug Dev. Res., 2006, vol. 67, pp. 154–165.
13. Marks, G.E., An aceto-carmine glycerol jelly for use in pollen-fertility counts, Biotech. Histochemþ, 1954, vol. 29, pp. 277–278.
14. Matsuura, H., On the secondary association of meiotic chromosomes in Tricyrtis latifolia MAX. and Dicentra spectabilis MIQ, J. Fac. Sci. Hokkaido Imp. Univ. Serþ, 1935, vol. 3, pp. 251–260.
15. Katayama, T., On so-called secondary association in rice plants. I Cytological observations, Japan. J. Genetþ, 1965, vol. 40, no. 1, pp. 25–32.
16. Chaghtai, S.A. and Hasan, Z., Effect of EMS, MES and MMS on the chromosomal behaviour of Lens esculenta during microsporogenesis, Biol. Plant. (Praha). 1979. V. 21. ¹ 4. P. 280–3.
17. Wills, A.B., Meiotic behaviour in the Brassiceae, Caryologia, 1966, vol. 19, no. 1, pp. 103–116.
18. Thomas, P.T. and Revell, S.H., Secondary association and heterochromatic attraction. I. Cicer arietinum, Ann. Bot., 1946, vol. 10, pp. 159–164.
19. Gustafsson, A., Primary and Secondary Association in Taraxacum, Hereditas XX, 1934.
20. Riley, R., The secondary pairing of bivalents with genetically similar chromosomes, Nature, 1960, vol. 185, pp. 751–752.
21. Hirayoshi, I., The chromosomal relationships in Oryzeae and Zizanieae, Cytologia Suppl. Vol. Proc. Int. Genet. Symposia, 1956, pp. 293–297.
22. Brown, S.W., Spurious secondary association and asymmetric spindles in a Luzula, Cytologia, 1950, vol. 15, nos. 3–4, pp. 259–268.
23. Propach, H., Cytogenetische untersuchungen in der gattung Solanum, Sect. Tuberarium. I. die sekundarpaarung, Zeitschrift fur Inductive Abstummungs-und Vererbungslehre, 1937, vol. 72, pp. 555–563.
24. Buxton, B.H. and Newton, W.C.F., Hybrids of Digitalis ambugua and Digitalis purpurea, their fertility and cytology, J. Genet., 1928, vol. 19, pp. 260–79.
25. Moffett, A.A., Cytological studies in cultivated pears, Genetica, 1934, vol. 15, pp. 511–518.
26. Stebbins, G.L., Variation and Evolution in Plants, New York: Columbia Univ. Press, 1950.
27. Bhattacharya, A. and Datta, A.K., Secondary chromosome associations in Uraria picta (Jacq.) DC. (Family: Leguminosae), Cytologia, 2010, vol. 75, pp. 37–40.
28. Olson, M., Bayesian procedures for discriminating among hypotheses with discrete distributions: inheritance in the tetraploid Astilbe Biternub, Genetics, 1997, vol. 147, pp. 1933–1942.
29. Lichter, P., Multilocular FISHing: what’s the catch?, Trends Genet., 1997, vol. 13, pp. 475–478.
30. Rao, N.B. and Lakshmi, N., Gamma ray induced meiotic abnormalities in Capsicum annum L., Caryologia, 1980, vol. 33, pp. 509–518.
31. Bala, S. and Gupta, R.C., Effect of secondary associations on meiosis, pollen fertility and pollen size in cape gooseberry (Physalis peruviana L.), Chromosome Bot., 2011, vol. 6, pp. 25–28.
32. Kumar, G. and Rai, P., EMS induced karyomorphological variations in maize (Zea mays L.) inbreds, Turk. J. Biol., 2007, vol. 3, pp. 187–195.
33. Gaulden, M.E., Hypothesis: some mutagens directly alter specific chromosomal proteins (DNA topoisomerase II and peripheral proteins) to produce chromosome stickiness, which causes chromosome aberrations, Mutagenesis, 1987, vol. 2, pp. 357–365.
34. Golubovskaya, I.N., Meiosis in maize: mei genes and conception of genetic control of meiosis, Adv. Genet., 1989, vol. 26, pp. 149–192.
35. Pagliarini, M.S., Meiotic behavior of economically important plant species: the relationship between fertility and male sterility, Genet. Mol. Biol., 2000, vol. 23, no. 4, pp. 997–1002.
36. Rana, P.K., Kumar, P.K., and Singhal, V.K., Spindle irregularities, chromatin transfer, and chromatin stickiness during male meiosis in Anemone tetrasepala (Ranunculaceae), Turk. J. Bot., 2013, vol. 37, pp. 167–176.
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