In silico analysis of the structure of variable domains of mouse single-chain antibodies specific to the human recombinant interferon β1b

Flyak A.I., Pavlova M.V., Gilchuk P.V.

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In silico analysis of the DNA encoding single-chain Fv antibodies (ScFv) specific to the human recombinant interferon b1b and a2b (rhIFN-b1b, rhIFN-a2b) has been carried out. The V-, D- and J-gene segments, the complementarity-determining (CDR) and framework (FR) regions, n-nucleotides as well as mutation rates which take place during the affinity maturation of the examined sequences have been determined. For the panel of ScFv against rhIFN-b1b isolated from an immune combinatorial cDNA library uniqueness of the CDRH3 loop by the length and amino acid composition has been shown. Multiple alignments with the nearest homologies from the NCBI databases have revealed that the sequences of ScFv obtained are new.

Key words: DNA sequencing, ScFv, variable domains, CDR, in silico analysis

Tsitologiya i Genetika 2009, vol. 43, no. 1, pp. 54-60

• National Taras Shevchenko University of Kyiv, Ukraine
• Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv

E-mail: gilchuk ukr.net, flyaka mail.ru

Flyak A.I., Pavlova M.V., Gilchuk P.V. In silico analysis of the structure of variable domains of mouse single-chain antibodies specific to the human recombinant interferon β1b, Tsitol Genet., 2009, vol. 43, no. 1, pp. 54-60.