Dynamics of genome changes in Rauwolfia serpentina callus cells upon the switch to conditions of submerged cultivation

Spiridonova E.V., Adnof D.M., Andreev I.O., Kunakh V.A.

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Genome of Rauwolfia serpentina callus cells was found to fail undergo the noticeable changes for several early passages upon the switch from surface to submerged cultivation in the liquid medium of special composition. After subsequent 4-6 passages in submerged culture RAPD spectra polymorphism was revealed which may reflect the changes in DNA sequence as well as in the structure of cell population that forms the strain. Introduction of the intermediary passage on the agar-solidified medium of more simple composition prior to transfer into liquid medium appeared not to affect essentially the level and the pattern of genome changes.

Key words: культура тканей растений, Rauwolfia serpentina, геном растений, RAPD-анализ

Tsitologiya i Genetika 2008, vol. 42, no. 2, pp. 35-41

E-mail: i.o.andreev imbg.org.ua

Spiridonova E.V., Adnof D.M., Andreev I.O., Kunakh V.A. Dynamics of genome changes in Rauwolfia serpentina callus cells upon the switch to conditions of submerged cultivation, Tsitol Genet., 2008, vol. 42, no. 2, pp. 35-41.