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Callose accumulation under treatment of tomato (Lycopersicon esculentum L.) cells with biotic elicitors
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Time-course of induced accumulation of callose in tomato cells has been studied. Localization of callose in L. esculenthum cells was investigated by fluorescent microscopy technique, and the optimal time for its determination was found. Callose accumulation in tomato cells treated with different biotic elicitors was determined. Non-linear dependence between callose accumulation and concentration of chitin oligomers (with 3–5 N-acetylglucosamine fragments) was established. Increasing of callose accumulation in tomato cells was proportional to the increase of chitin dimer and chitosan in the culture medium.
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