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Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior
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Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium-mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification scheme has been developed and included ammonium sulfate precipitation and Q-sepharose anion-exchange chromatography. It results in obtaining of a fraction with about 85 % GFP homogeneity and the protein yield of about 75 %.
Key words: Nicotiana excelsior; Agrobacterium tumefaciens; transient expression; recombinant protein; GFP
|Coded & Designed by Volodymyr Duplij||Modified 15.06.21|