Chromosome variability in the tissue culture of rare Gentiana species

Twardovska M.O., Strashniuk N.M., Mel’nyk V.M., Adonin V.I., Kunakh V.A.

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Cytogenetic analysis of plants and tissue culture of Gentiana lutea, G.punctata, G.acaulis has been carried out. Culturing in vitro was found to result in the changes of chromosome number in the calluses of the species involved. Species specificity for variation of the cultured cell genomes was shown. Contribution of the original plant genotypes to the cytogenetic structure of the tissue culture was established. Gentiana callus tissues (except for in vitro culture of G.punctata, derived from plant of Breskul’ska population) were found to exhibit modal class with the cells of diploid and nearly diploid chromosome sets.

Key words: plant tissue culture, Gentiana L., chromosomal variability in vitro

Tsitologiya i Genetika 2008, vol. 42, no. 4, pp. 12-17

E-mail: kunakh imbg.org.ua, strashniuk mail.ru

Twardovska M.O., Strashniuk N.M., Mel’nyk V.M., Adonin V.I., Kunakh V.A. Chromosome variability in the tissue culture of rare Gentiana species, Tsitol Genet., 2008, vol. 42, no. 4, pp. 12-17.