Представлено результати поверхневої функціоналізації за допомогою бичачого сироваткового альбуміну напівпровідникових наночастинок (квантових точок) Ag2S, отриманих методом «зеленого» синтезу. Виявлено, що внаслідок функціоналізації максимум люмінесценції квантових точок Ag2S зсувається до короткохвильового діапазону (піки у межах 270–350 нм). У результаті вивчення морфології та розмірів функціоналізованих квантових точок Ag2S встановлено, що для них є характерною однорідна поверхня без структурних дефектів та сферична форма. Діаметр функціоналізованих квантових точок становив приблизно 20 нм, тоді як діаметр квантових точок без функціоналізації – до 10 нм. Отримано кон’югати функціоналізованих квантових точок Ag2S з моноклональними антитілами (на прикладі антитіл TU-01 проти α-субодиниці тубуліну – основного білка мікротрубочок). У результаті спектрофотометричного аналізу виявлено, що спектр люмінесценції отриманих біокон’югатів Ag2S – TU-01 мав декілька максимумів залежно від збудження. Максимуми відповідали діапазону довжин хвиль 410–470 нм. Продемонстровано, що отримані кон’югати Ag2S – TU-01 здатні візуалізувати мікротрубочки, зокрема в клітинах Nicotiana tabacum. Отже, нами вперше створено біокон’югати з моноклональними антитілами за використання квантових точок Ag2S, отриманих методом «зеленого синтезу», який суттєво знижує токсичність таких наночастинок, що дає можливість розглядати їх як ефективні люмінісценті зонди в клітинно-біологічних дослідженнях.
Ключові слова: Ag2S, квантові точки, функціоналізація, моноклональні антитіла, біокон’югація, спектрофотометрія, мікротрубочки
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