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Down regulation of Potato Virus Y (PVY) Coat Protein (CP) expression by Iberis gibraltarica protein extract

Bilal M., Tabassum B., Ali Q., Nasir I.A.

Реферат статті 




РЕЗЮМЕ. Y-вірус картоплі (PVY) є одним з найдеструктивніших вірусів картоплі і значною загрозою для її ефективного вирощування у всьому світі. Майже 70 % втрат урожаю картоплі спричинені раннім зараженням PVY. Ген білка оболонки (CP) відіграє значну роль в адаптації господаря до більшості вірусів, що вражають рослини. Білок оболонки у PVY та інших потивірусів почали вивчати все частіше та інтенсивніше з метою створення резистентності до них у різних рослин. У цьому дослідженні ми також вивчали ген CP та оцінювали in vitro потенціал екстракта загального білка Iberis gibraltarica у боротьбі проти експресії гена CP. Було виділено загальний білок Iberis gibraltarica з подальшою оцінкою його кількісних характеристик. Повнорозмірний ген CP ампліфікували з листків картоплі, інфікованої PVY, і клонували в екпресійний вектор ссавців. Далі його трансфектували на лінію клітин ссавців для отримання транзіентної експресії, експресію мРНК гена CP порівняно з загальним білком Iberis gibraltarica аналізували за допомогою ПЛР у реальному часі. Результати показали, що загальний білок Iberis gibraltarica знижує рівень експресії мРНК у in vitro дослідженнях більше, ніж на  90%.

Ключові слова: Iberis gibraltarica, Y-вірус картоплі, білок оболонки, пкДНК 3.1 (+)

Цитологія і генетика 2021, том 55, № 1, C. 93-95

  1. Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan
  2. Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
  3. Insitute of Molecualr Biology and Biotechnology,  Lahore University, Lahore, Pakistan

E-mail: bilal.camb pu.edu.pk, saim1692 gmail.com

Bilal M., Tabassum B., Ali Q., Nasir I.A. Down regulation of Potato Virus Y (PVY) Coat Protein (CP) expression by Iberis gibraltarica protein extract, Цитологія і генетика., 2021, том 55, № 1, C. 93-95.

В "Cytology and Genetics". Якщо тільки можливо, цитуйте статтю по нашій англомовній версії:
Muhammad Bilal, Bushra Tabassum, Qurban Ali & Idrees Ahmad Nasir Down Regulation of Potato Virus Y (PVY) Coat Protein (CP) Expression by Iberis gibraltarica Protein Extract, Cytol Genet., 2021, vol. 55, no. 1, pp. 80–86
DOI: 10.3103/S0095452721010102


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