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Detection of alkalilabile sites on satellite DNA by DNA breakage coupled with fluorescence in situ hybridization (DNAFISH) monitor DNA damage in cervical epithelial cells

Garcíavielma C., Cortésgutiérrez E.I., García Salas J.A., Dávilarodríguez M.I.

 




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2020, 54, 2, C. 80-82

  1. Department of Genetics. Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, IMSS. 64720 Monterrey, México
  2. Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey, México

E-mail: :elvacortes cibinmty.net

Garcíavielma C., Cortésgutiérrez E.I., García Salas J.A., Dávilarodríguez M.I. Detection of alkalilabile sites on satellite DNA by DNA breakage coupled with fluorescence in situ hybridization (DNAFISH) monitor DNA damage in cervical epithelial cells, ., 2020, 54, 2, C. 80-82.

"Cytology and Genetics". , :
C. Garcia-Vielma, E. I. Cortés-Gutiérrez, Juan Antonio Garcia Salas & M. I. Dávila-Rodriguez Detection of Alkali-Labile Sites on Satellite DNA by DNA Breakage Coupled with Fluorescence in Situ Hybridization (DNA-FISH) Monitor DNA Damage in Cervical Epithelial Cells, Cytol Genet., 2020, vol. 54, no. 2, pp. pages1731
DOI: 10.3103/S0095452720020061


1. Tyler-Smith, C. and Willard, H.F., Mammalian chromosome structure, Curr. Opin. Genet. Dev., 1993, vol. 3, no. 3, pp. 390397. https://doi.org/10.1016/0959-437x(93)90110-b

2. Meyne, J., Goodwin, E.H., and Moyzis, R.K., Chromosome localization and orientation of the simple sequence repeat of human satellite I DNA, Chromosoma, 1994, vol. 103, no. 2, pp. 99103. https://doi.org/10.1007/bf00352318

3. Albright, K.L., Bartholdi, M.F., Cram, L.S., Deaven, L.L., Hildebrand, C.E., Joste, E.N., Longmire, J.L., Meyne, J., and Schwarzacher-Robinson, T., Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis, Chromosoma, 1987, vol. 95, no. 6, pp. 375386. https://doi.org/10.1007/bf00333988

4. Jeanpierre, M., Human satellite 2 and 3, Ann. Génét., 1994, vol. 37, no. 4, pp. 163171.

5. Aze, A., Sannino, V., Soffientini, P., Bachi, A., and Costanzo, V., Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression, Nat. Cell Biol., 2016, vol. 18, no. 6, pp. 86848691. https://doi.org/10.1038/ncb3344

6. Fernández, J.L., Goyanes, V., Ramiro-Diaz, J., and Gosálvez, J., Application of FISH for in situ detection and quantification of DNA breaks, Cytogenet. Cell Genet., 1998, vol. 82, nos 3-4, pp. 251256. https://doi.org/10.1159/000015112

7. Cortés-Gutiérrez, E.I., Dávila-Rodríguez, M.I., Fernandez, J.L., López-Fernández, C., and Gosálvez, J., DNA damage in women with cervical neoplasia evaluated by DNA breakage detection-fluorescence in situ hybridization, Anal. Quant. Cytol. Histol., 2011, vol. 33, no. 3, pp. 175181.

8. Vogt, P., Code domains in tandem repetitive DNA sequence structures, Chromosoma, 1992, vol. 101, no. 10, pp. 585589. https://doi.org/10.1007/bf00360534

9. Fernández, J.L., Valverde, D., Goyanes, V., Buño, I., and Gosálvez, J., Alu I in situ digestion of human alphoid and classical satellite DNA regions: high-resolution digital image analysis of FISH signals from condensed and extended chromatin, Cytogenet. Cell Genet., 1997, vol. 76, nos. 12, pp. 94100. https://doi.org/10.1159/000134522

10. Gosálvez, J., López-Fernández, C., Goyanes, V., and Mezzanotte, R., Chromosome differentiation using nucleases: an overview, in Chromosomes Today, Henriques-Gil, N., Parker, J.S., and Puertas, M.J., Eds., London: Chapman and Hall, 1997, vol. 12, pp. 2349. https://doi.org/10.1007/978-94-009-1537-4_2

11. Darzynkiewicz, Z., Huang, X., and Okafuji, M., Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication), Methods Mol. Biol., 2006, vol. 314, pp. 8193. https://doi.org/10.1385/1-59259-973-7:081

12. Gore, J., Bryant, Z., Nollmann, M., Le, M.U., Cozzarelli, N.R., and Bustamante, C., DNA overwinds when stretched, Nature, 2006, vol. 442, no. 7104, pp. 836839. https://doi.org/10.1038/nature04974

13. Fernández, J.L., Vázquez-Gundín, F., Rivero, M.T., Genescá, A., Gosálvez, J., and Goyanes, V., DBD-FISH on neutral comets: simultaneous analysis of DNA single- and double-strand breaks in individual cells, Exp. Cell Res., 2001, vol. 270, no. 1, pp. 102109. https://doi.org/10.1007/978-1-60327-409-8_11

14. Cortés-Gutiérrez, E.I., Ortíz-Hernández, B.L., Dávila-Rodríguez, M.I., Cerda-Flores, R.M., Fernández, J.L., López-Fernández, C., and Gosálvez, J., 5-bp Classical satellite DNA loci from chromosome-1 instability in cervical neoplasia detected by DNA breakage detection/fluorescence in situ hybridization (DBD-FISH), Int. J. Mol. Sci., 2013, vol. 14, no. 2, pp. 41354147. https://doi.org/10.3390/ijms14024135

15. Cortés-Gutiérrez, E.I., Dávila-Rodríguez, M.I., Fernández, J.L., López-Fernández, C., and Gosálvez, J., Koilocytes are enriched for alkaline-labile sites, Eur. J. Histochem., 2010, vol. 54, no. 4. e32. https://doi.org/10.4081/ejh.2010.e32

16. Fernández, J.L., Vazquez-Gundin, F., Rivero, M.T., Goyanes, V., and Gosálvez, J., Evidence of abundant constitutive alkali-labile sites in human 5 bp classical satellite DNA loci by DBD-FISH, Mutat. Res., 2001, vol. 473, no. 2, pp. 163168. https://doi.org/10.1016/s0027-5107(00)00146-9

17. Cortés-Gutiérrez, E.I., Dávila-Rodríguez, M.I., López-Fernández, C., Fernández, J.L., and Gosálvez, J., Alkali-labile sites in sperm cells from Sus and Ovis species, Int. J. Androl., 2008, vol. 31, no. 3, pp. 354363. https://doi.org/10.1111/j.1365-2605.2007.00781.x

18. Vazquez-Gundin, F., Goyanes, V., Campos, A., Blasco, M., Gosálvez, J., and Fernández, J.L., High frequency of constitutive alkali-labile sites in mouse major satellite DNA, detected by DNA breakage detection-fluorescence in situ hybridization, Mutat. Res., 2001, vol. 483, nos. 12, pp. 4350. https://doi.org/10.1016/s0027-5107(01)00218-4

19. Rivero, M.T., Mosquera, A., Goyanes, V., Slijepcevic, P., and Fernández, J.L., Differences in repair profiles of interstitial telomeric sites between normal and DNA double-strand ALS in mammalian sperm break repair deficient Chinese hamster cells, Exp. Cell Res., 2004, vol. 295, no. 1, pp. 161172. https://doi.org/10.1016/j.yexcr.2003.12.031

20. López-Fernández, C., Arroyo, F., Fernández, J.L., and Gosálvez, J., Interstitial telomeric sequence blocks in constitutive pericentromeric heterochromatin from Pyrgomorpha conica (Orthoptera) are enriched in constitutive alkali-labile sites, Mutat. Res., 2006, vol. 599, nos. 12, pp. 3644. https://doi.org/10.1016/j.mrfmmm.2006.01.004

21. Cortés-Gutiérrez, E.I., López-Fernández, C., Fernández, J.L., Dávila-Rodríguez, M.I., Johnston, S.D., and Gosálvez, J., Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay, Front. Genet., 2014, vol. 27, no. 5, p. 404. https://doi.org/10.3389/fgene.2014.00404

22. Von Sonntag, C., Carbohydrate radicals: from ethylene glycol to DNA strand breakage, Int. J. Radiat. Biol., 2014, vol. 90, no. 6, pp. 416422. https://doi.org/10.3109/09553002.2014.908040

23. Bandyopadhyay, R., Berend, S.A., Page, S.L., Choo, K.H., and Shaffer, L.G., Satellite III sequences on 14p and their relevance to Robertsonian translocation formation, Chromosome Res., 2001, vol. 9, no. 3, pp. 235242. https://doi.org/10.1023/a:1016652621226

24. Lana, E., Megarbane, A., Tourriere, H., Sarda, P., Lefranc, G., Claustres, M., and De Sario, A., DNA replication is altered in Immunodeficiency Centromeric instability Facial anomalies (ICF) cells carrying DNMT3B mutations, Eur. J. Hum. Genet., 2012, vol. 20, no. 1, pp. 10441050. https://doi.org/10.1038/ejhg.2012.41

25. Jefferson, A., Colella, S., Moralli, D., Wilson, N., Yusuf, M., Gimelli, G., Ragoussis, J., and Volpi, E.V., Altered intra-nuclear organisation of heterochromatin and genes in ICF syndrome, PLoS One, 2010, vol. 5, no. 6. e11 364. https://doi.org/10.1371/journal.pone.0011364

26. Toubiana, S., Velasco, G., Chityat, A., Kaindl, A.M., Hershtig, N., Tzur-Gilat, A., and Francastel, C., Subtelomeric methylation distinguishes between subtypes of Immunodeficiency. Centromeric Instability and Facial Anomalies (ICF) syndrome, Hum. Mol. Genet., 2018, vol. 27, no. 20, pp. 35683581. https://doi.org/10.1093/hmg/ddy265

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