Цитологія і генетика 2023, том 57, № 5, 82-85

Cytology and Genetics 2023, том 57, № 5, 466–490, doi: https://www.doi.org/https://doi.org/10.3103/S009545272305002X

Computational identification of Citrus reticulata L. microRNAs and the cis-acting regulatory elements to predict the expression probability of their respective MIR genes

Choudhury H.D., Rajwanshi R.

Department of Biotechnology, Assam University Silchar, India
Discipline of Life Sciences, School of Sciences, Indira Gandhi National Open University, New Delhi, India

РЕЗЮМЕ. МікроРНК (miRNA), невеликий клас некодуючих РНК, який регулює експресію генів, обмежені декількома видами рослин. У цьому дослідженні було застосовано підхід insilico з метою визначення мікроРНК у відомих маркерних експресованих послідовностях (EST) Citrus reticulata L. Загалом було передбачено 17 мікроРНК із 23 різних EST, а також їхні вторинні структури та цілі. До ідентифікованих 63 цілей входять декілька транскрипційних факторів, білки, що регулюють ріст, розвиток, цвітіння рослин та розвиток насіння, а також реакцію на стрес. Цисрегуляторний елемент, присутній у промотерній ділянці MIR генів C. reticulata, продемонстрував свою актуальність щодо реакції на світло, ауксин, гібберелліни, абсцизову кислоту (ABA), антоцианін, саліцилову кислоту, щодо анаеробної індукції, контролю циркадного ритму, нітратозалежного регулювання клітинного циклу та реплікації ДНК, захисту та реакції на стрес. У цьому дослідженні було встановлено мікроРНК та їхні регуляторні елементи в C. reticulate. Наше дослідження також буде корисним для вивчення можливості ідентифікації мікроРНК на основі геномних даних різних рослин та передбачення можливості експресії ідентифікованих генів мікроРНК на основі наявності висхідного промотера та інших регуляторних елементів.

Ключові слова: мікроРНК, Citrus reticulata, маркерна експресована послідовність, цисрегуляторний елемент, psRNATarget

Цитологія і генетика
2023, том 57, № 5, 82-85

Cytology and Genetics
2023, том 57, № 5, 466–490,
doi: https://doi.org/10.3103/S009545272305002X

Повний текст та додаткові матеріали

Цитована література

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