Біобутанол – один із видів біопалива, який одержують за допомогою мікробного синтезу (ацетон-бутанол-етанольна ферментація). Підвищити рівень накопичення цільового продукту можна за рахунок метаболічних змін у клітинах-продуцентах за умов впливу стресових факторів. З цією метою було досліджено вплив на функціонування клітин Clostridium sp. таких стресових чинників, як алопуринол, фурфурол, цинк, кисень, а також гліцеролу як неспецифічного субстрату. Показано, що концентрація бутанолу в порівнянні з контролем (0,6 г/л) була найвищою (1,2 г/л) за умов додавання 0,5 г/л фурфуролу в культуральне середовище. Використання ZnSO4 в концентрації 0,001 г/л підвищувало концентрацію бутанолу до 1,1 г/л, а найбільшу концентрацію бутанолу (1,3 г/л) було отримано при використанні глюкози та гліцеролу як джерел вуглецю у співвідношенні 1 : 3. Концентрація бутанолу у культуральному середовищі підвищувалась до 0,8 г/л за умов додавання 0,025 г/л алопуринолу. Обґрунтовано можливість використання вещезгаданих стресових чинників для підвищення накопичення цільового продукту за рахунок впливу на метаболічні шляхи у клітинах мікроорганізмів.
Ключові слова: Clostridium, штам продуцент, бутанол, стресові фактори, алопуринол, фурфурол, цинк, кисень, гліцерол
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Цитована література
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