Цитологія і генетика 2022, том 56, № 2, 75-77
Cytology and Genetics 2022, том 56, № 2, 185–195, doi: https://www.doi.org/10.3103/S0095452722020116

MELATONIN IMPROVES LEFT VENTRICULAR MITOCHONDRIAL DYNAMICS IN RATS

Uzun M., Oztopuz R.O., Eroglu H.A., Doganlar O., Doganlar Z.B., M.A. Ovali, Demir U., Buyuk B.

  1. Faculty of Medicine, Department of Physiology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
  2. Faculty of Medicine, Department of Biophysics, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
  3. Faculty of Medicine, Department of Medical Biology, Trakya University, Edirne, Turkey
  4. Experimental Research Center, Çanakkale Onsekiz Mart University, Çanakkale, Turkey
  5. Faculty of Medicine, Department of Histology-Embryology, İzmir Democracy University, İzmir, Turkey

РЕЗЮМЕ. Зростає усвідомлення того, що ефективна та регулярна динаміка мітохондрій покращує функцію серця і впливає на якість життя. Мелатонін – це основний гормон епіфізу, який оптимізує динаміку мітохондрій при багатьох порушеннях серцевої діяльності. З огляду на цей факт, ми вводили здоровим щурам мелатонін упродовж усієї доби, щоб дослідити зміни у динаміці мітохондрій лівого шлуночка серця і наприкінці ночі, і протягом дня. Двадцять самців лінії Вістар (віком 3–4 міс) були рандомізовано розподілені у групу контролю (C; n = 10) та групу мелатоніну (MEL; додавання 10 мг/кг мелатоніну до питної води, n = 10). На п’ятий день дослідження рандомізовано обрали по 5 щурів з груп та піддали їх евтаназії о 08:00, а інших тварин, по 5 щурів з обох груп, умертвили о 20:00 і взяли зразки тканини з лівого шлуночка (LV). Результати кількісної ПЦР у реальному часі і Вестерн-блот аналізу продемонстрували, що мелатонін відіграє превентивну роль у злитті мітохондрій та мітофагії по осі DRP1/FIS1 та BNIP3/NIX, відповідно. Крім того, введення мелатоніну значно знизило активацію P21, індукувало зупинку клітинного циклу, P27, зрештою, регулювало сигнали каспазо-залежного мітохондріального апоптозу залежно від часу. Наші результати показують, що мелатонін може бути терапевтичним засобом захисту біоенергетичної динаміки мітохондрій у серці.

Ключові слова: мелатонін, серце, апоптоз, динаміка мітохондрій, мітофагія

Цитологія і генетика
2022, том 56, № 2, 75-77

Current Issue
Cytology and Genetics
2022, том 56, № 2, 185–195,
doi: 10.3103/S0095452722020116

Повний текст та додаткові матеріали

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