РЕЗЮМЕ. Цитоміксис – це загадкове цитологічне явище, яке спостерігається між близько розташованими материнськими клітинами пилку (МКП) Cuminum cyminum L. Взаємне переміщення складу хроматину між двома прилеглими мейоцитами спостерігали при різних рівнях гаммаопромінення viz. 100, 125,
150 і 175 Гр у сорту зіри GC4. Дослідження мікроспорогенезу наборів, опромінених гаммапроменями, виявило міжклітинну міграцію різними каналами або безпосереднє злиття. У цій роботі цитоміксис набагато частіше спостерігали як мейоз I порівняно з пізнішим етапом, тобто мейозом II. Рівень повторюваності цитоміксису демонструє поступове зростання з одночасним збільшенням дози гаммаопромінення. Внаслідок цитоміксису було зафіксовано різні аномальні продукти постмейозу на пізнішій стадії клітинного циклу. Більше того, окрім цитоміксису, спостерігалися також інші хромосомні аномалії, які призвели до зниження родючості пилку. Синцитії спостерігали при низькій частоті, однак вони мали високу еволюційну значимість, оскільки вони виробляють гамети з високим рівнем плоїдності. Утворення гамет з незбалансованих хромосом можна використовувати надалі у технологіях селекції, оскільки вони є потужним індуктором змін.
Ключові слова: цитоміксис, поєднання цитоплазми, Cuminum cyminum, гаммапромені, гіперплоїдний, гіпоплоїдний, синцитій
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Bellucci, M., Roscini, C., and Mariani, A., Cytomixis in the pollen mother cells of Medicago sativa L., J. Hered., 2003, vol. 94, no. 6, pp. 512–516. https://doi.org/10.1093/jhered/esg096
Bhat, T.A., Parveen, S., and Khan, A.H., MMS-induced cytomixis in pollen mother cells of broad bean (Vicia faba L.), Turk. J. Bot., 2006, vol. 30, pp. 273–279.
Bione, N.C.P., Pagliarini, M.S., and de Toleredo, J.F.F., Meiotic behavior of several Brazilian soybean varieties, Genet. Mol. Biol., 2000, vol. 23, no. 3, pp. 623–631. https://doi.org/10.1590/S1415-47572000000300022
Boldrini, K.R., Pagliarini, M.S., and Valle, C.B., Cell fusion and cytomixis during microsporogenesis in Brachiaria humidicola (Poaceae), S. Afr. J. Bot., 2006, vol. 72, pp. 478–481. https://doi.org/10.1016/j.sajb.2005.11.004
Datta, A.K., Mukherjee, M., and Iqbal, M., Persistent cytomixis in Ocimum basilicum L. (Lamiaceae) and Withania somnifera (L.) Dun (Solanaceae), Cytologia, 2005, vol. 70, no. 3, pp. 309–313. https://doi.org/10.1508/cytologia.70.309
Dwivedi, H. and Kumar, G., Induced syncyte formation via cytomixis in Trachyspermum ammi (L.) Sprague (Apiaceae), Caryologia, 2018, vol. 71, no. 4, pp. 420–427. https://doi.org/10.108/00087114.2018.1499480
Ghaffari, S.M., Occurrence of diploid and polyploidy microspores in Sorghum bicolor (Poaceae) is the result of cytomixis, Afr. J. Biotechnol., 2006, vol. 5, pp. 1450–1453.
Guan, J.Z., Wang, J.J., Cheng, Z.H., and Li, Z.Y., Cytomixis and meiotic abnormalities during microsporogenesis are responsible for male sterility and chromosome variations in Houttuynia cordata, Genet. Mol. Res., 2012, vol. 11, pp. 121–130. https://doi.org/10.4238/2012pp
Guo, G.Q. and Zheng, G.C., Hypothesis for functions of intercellular bridges in male germ cell development and its cellular mechanisms, J. Theor. Biol., 2004, vol. 229, no. 1, pp. 139–146. https://doi.org/10.1016/j.jtbi.2004.03.010
Haroun, S.A., Al Shehr, A.M., and Al Wadie, H.M., Cytomixis in the microsporogenesis of Vicia faba L. (Fabaceae), Cytologia, 2004, vol. 69, pp. 7–11. https://doi.org/10.1508/cytologia.69.7
Kornicke, M., Uber ortsveranderung von Zellkarnern SB, Niederhein Ges Natur-U Heilkunde, 1901, pp. 14–25.
Kravets, E.A., Cellular and tissue mechanisms of recovery processes in Hordeum distichum L. under irradiation, Cytol. Genet., 2009, vol. 43, pp. 9–17. https://doi.org/10.3103/S0095452709010022
Kravets, E.A., Nature, significance, and cytological consequences of cytomixis, Cytol. Genet., 2012, vol. 46, pp. 188–195. https://doi.org/10.3103/S0095452712030061
Kravets, E.A., Cytomixis as a primary form of sexual process, Adv. Cytol. Pathol., 2018, vol. 3, no. 4, pp. 88–91. https://doi.org/10.15406/acp.2018.03.00059
Kumar, P., Singhal, V.K., Cytology of Caltha palustris L. (Ranunculaceae) from cold regions of Western Himalayas, Cytologia, 2008, vol. 73, pp. 137–143. https://doi.org/10.1508/cytologia.73.137
Kumar, G., and Singh, S., Induced cytomictic crosstalk behaviour among micro-meiocytes of Cyamopsis tetragonoloba (L.) Taub.(cluster bean): Reasons and repercussions, CARYAB, 2020, vol. 73, no. 2, pp. 111–119. https://doi.org/10.13128/caryologia-544
Kumar, P., Singhal, V.K., Male meiosis, morphometric analysis and distribution pattern of 2× and 4× cytotypes of Ranunculus hirtellus Royle, 1834 (Ranunculaceae) from the cold regions of northwest Himalayas (India), Comp. Cytogenet., 2011, vol. 5, pp. 143–161. https://doi.org/10.3897/CompCytogen.v5i3.1359
Kunakh, V.A., Ontogenetic plasticity of the genome as the basis of plant adaptability, Zhebrakovskie Chteniya III (3d Int. Sci. Conf. “Zhebrakov readings”), Minsk: Pravo Ekon., 2011.
Larrosa, F.H., Maune, J.F., Erazzu, L.E., and Camadro, E.L., Meiotic abnormalities underlying pollen sterility in wild potato hybrids and spontaneous populations, Plant Biol., 2012, vol. 14, pp. 223–233. https://doi.org/10.1111/j.1438-8677.2011.00470.x
Lattoo, S.K., Khan, S., Bamotra, S., and Dhar, A.K., Cytomixis impairs meiosis and influences reproductive success in Chlorophytum comosum (Thunb.) Jacq.—An additional strategy and possible implications, J. Biosci., 2006, vol. 31, pp. 629–637. https://doi.org/10.1007/BF02708415
Liu, H., Guo, G.Q., He, Y.K., Lu, Y.P., and Zheng, G.C., Visualization on intercellular movement of chromatin in intact living anthers of transgenic tobacco expressing histone 2BCFP fusion protein, Caryologia, 2007, vol. 60, nos. 1–2, pp. 1–20. https://doi.org/10.1080/00087114.2007.10589542
Lone, F.A. and Wafai, B.A., Chromsome conspectus and cytogenetic appraisal of some commercial cultivars of cherry (Prunus avium L., P. cerasus L.) and plum (P. domestica L.) using a partially modified staining technique, Phytomorphology, 2009, vol. 59, no. 1, pp. 29–34.
Malallah, G.A. and Talaat, A.A., Cytomixis and its possible evolutionary role in a Kuwaiti population of Diplotaxis harra (Brassicaceae), Bot. J. Linn. Soc., 2003, vol. 143, pp. 169–175. https://doi.org/10.1046/j.1095-8339.2003.00218.x
Malallah, G.A., Cytomixis and Its Possible Evolutionary Role in a Kuwaiti Population of Diplotaxis harra (Brassicaceae), Comp. Cytogenet., 2011, vol. 5, pp. 143–161.
Mamun, E.A., Alfred, S., Cantrill, L.C., Overall, R.L., and Sutton, B.G., Effects of chilling on male gametophyte development in rice, Cell Biol. Int., 2006, vol. 30, no. 7, pp. 583–591. https://doi.org/10.1016/j.cellbi.2006.03.004
Mursalimov, S.R. and Deineko, E.V., An ultrastructural study of microsporogenesis in tobacco line SR1, Biologia, 2012, vol. 67, pp. 369–376. https://doi.org/10.2478/s11756-012-0005-1
Mursalimov, S., Sidorchuk, Y., and Deineko, E., The role of spherosome like vesicles information of cytomictic channels between tobacco microsporocytes, Biol. Plant., 2013, vol. 57, pp. 291–297. https://doi.org/10.1007/s10535-012-0276-y
Mursalimov, S.R. and Deineko, E.V., How cytomixis can form unreduced gametes in tobacco, Plant Syst. Evol., 2015, vol. 301, pp. 1293–1297. https://doi.org/10.1007/s00606-014-1150-5
Nirmala, C. and Kaul, M.L.H., Male sterility in pea VI. Gene action duplicity, Cytologia, 1994, vol. 59, pp. 195–201.
Pagliarini, M.S., Meiotic behaviour of economically important plant species: the relationship between fertility and male sterility, Genet. Mol. Biol., 2000, vol. 23, pp. 997–1002. https://doi.org/10.1590/S1415-47572000000400045
Papihin, R.V. and Yandovka, L.F., Cytomixis during microsporogenesis in Cerasus fruticosa Pall, Biomed. Pharmacol. J., 2014, vol. 7, pp. 473–479. https://doi.org/10.13005/bpj/514
Peng, Z.H.S., Yang, J. and Zheng, G.C.H., Cytomixis in pollen mother cells of new synthetic hexaploid amphidiploid (Aegilops tauschii, Triticum turgidum), Cytologia, 2003, vol. 68, pp. 335–340. https://doi.org/10.1508/cytologia.68.335
Popova, A.F., Ivanenko, G.F., Ustinova, A.Yu., and Zaslavsky, V.A., Localization of callose in microspores and pollen grains in Sium latifolium L. plants in different water regimes, Cytol. Genet., 2008, vol. 42, pp. 363–368. https://doi.org/10.3103/S0095452708060017
Ramanna, M.S. and Jacobsen, E., Relevance of sexual polyploidization for crop improvement—A review, Euphytica, 2003, vol. 133, pp. 3–18. https://doi.org/10.1023/A:1025600824483
Ramsey, J. and Schemske, D.W., Neopolyploidy in flowering plants, Annu. Rev. Ecol. Syst., 2002, vol. 33, pp. 589–639. https://doi.org/10.1146/annurev.ecolsys.33.010802.150437
Ranjbar, M., Karamian, R., and Nouri, S., Impact of cytomixis on meiosis in Astragalus cyclophyllos Beck (Fabaceae) from Iran, Caryologia, 2011, vol. 64, no. 3, pp. 256–264. https://doi.org/10.1080/00087114.2011.10589791
Reis, A.C., Sousa, S.M., Vale, A.A., Pierre, P.M.O., Franco, A.L., Campos, J.M.S., Viera, R.F., and Viccini, L.F., Lippia alba (Verbenaceae): A new tropical autopolyploid complex?, Am. J. Bot., 2014, vol. 101, pp. 1002–1012. https://doi.org/10.3732/ajb.1400149
Saraswathy Amma, C.K., Namboodiri, A.N., Panikkar, A.O.N., and Sethuraj, M.R., Radiation induced male sterility in Hevea brasiliensis (Willd. ex Adr. De Juss.) Muell. Arg, Cytologia, 1990, vol. 55, no. 4, pp. 547–551. https://doi.org/10.1508/cytologia.55.547
Shkutina, F.M. and Kozlovskaya, V.F., Cytomixis in meiosis of some hybrid forms of cereals of the Triticinae subtribe, Genetics, 1974, vol. 10, pp. 5–12.
Sidorchuk, Yu.V., Deineko, E.V., and Shumnyi, V.K., Characteristics of the cytomixis in the pollen mother cells of transgenic tobacco plants (Nicotiana tabacum L.) with mutant phenotype, Tsitologya, 2007, vol. 49, no. 10, pp. 870–875.
Singhal, V.K., Gill, B.S., and Dhaliwal, R.S., Status of chromosomal diversity in the hardwood tree species of Punjab State, Cytol. Genet., 2007, vol. 8, pp. 67–83.
Singhal, V.K. and Kumar, P., Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeate Royle), J. Biosci., 2008, vol. 33, pp. 371–380.
Singhal, V.K., Kaur, S., and Kumar, P., Aberrant male meiosis, pollen sterility and variable sized pollen grains in Clematis Montana Buch.-Ham. ex DC. from Dalhousie hills, Himachal Pradesh, Cytologia, 2010, vol. 75, no. 1, pp. 31–36. https://doi.org/10.1508/cytologia.75.31
Singhal, V.K., Gill, B.S., and Dhaliwal, R.S., Status of chromosomal diversity in the hardwood tree species of Punjab State, Cytol. Genet., 2007, vol. 8, pp. 67–83.
Soltis, D.E., Pires, J.C., Kovarik, A., Tate, J., and Mavrodiev, E., Recent and recurrent polyploidy in Tragopogon (Asteraceae): cytogenetic, genomic and genetic comparisons, Biol. J. Linn. Soc., 2004, vol. 82, no. 4, pp. 485–501. https://doi.org/10.1111/j.1095-8312.2004.00335.x
Wang, S.Yu., Yu, Ch.H., Li, S., Wang, Ch.Y., and Zheng, G.C., Ultrastructural aspects and possible origin of cytoplasmic channels providing intercellular connection in vegetative tissues of anthers, Russ. J. Plant Physiol., 2004, vol. 51, pp. 97–106. https://doi.org/10.1023/B:RUPP.0000011308.61339.fe
Yu, C.H., Guo, G.Q., Nie, X.W., and Zheng, G.C., Cytochemical localization of pectinase activity in pollen mother cells of tobacco during meiotic prophase I and its relation to the formation of secondary plasmodesmata and cytoplasmic channels, Acta Bot. Sin., 2004, vol. 46, no. 12, pp.143–145.
Zhou, S.Q., Viewing the difference between the diploid and the polyploid in the light of the upland cotton aneuploid, Hereditas, 2003, vol. 138, pp. 65–72. https://doi.org/10.1034/j.1601-5223.2003.01689.x