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Цитологія і генетика 2022, том 56, № 2, 21-37
Cytology and Genetics 2022, том 56, № 2, 125–138, doi: https://www.doi.org/10.3103/S0095452722020086

Вплив імплантації фібринового матриксу, асоційованого з клітинами неонатального головного мозку, на перебіг експериментальної спінальної травми

Медведєв В.В., Олексенко Н.П., Пічкур Л.Д., Вербовська С.А., Савосько С.І., Драгунцова Н.Г., Лонтковський Ю.А., Васлович В.В., Цимбалюк В.І.

  1. Національний медичний університет імені О.О. Богомольця, бульвар Тараса Шевченка, 13, Київ, 01601, Україна
  2. ДУ «Інститут нейрохірургії ім. акад. А.П. Ромоданова НАМН України», 04050, вул. Платона Майбороди, 32, Київ, Україна
  3. КНП «Кам’янець­Подільська міська лікарня» вул. Пушкінська, 31, Кам’янець­Подільський, Хмельницька область, Україна
  4. Національна академія медичних наук України, вул. Герцена, 12, Київ, 04050, Україна

Досліджено вплив негайної імплантації фібринового матриксу, асоційованого з алогенними клітинами неонатального головного мозку щура, на відновлення рухової функції після травми спинного мозку (ТрСМ). Тварини – дорослі білі безпородні щури (~260 г, 4–5 місяців. (Модель ТрСМ – лівобічний перетин половини спинного мозку на рівні сегментів ~Т13–L1. Відновне лікування – негайна трансплантація у зону травми фібринового матриксу людини, асоційованого з клітинами неонатального головного мозку щура (neonatal brain cells, NBC, n = 9). Референтні групи – ізо-льована ТрСМ (trauma, Tr, n = 7) та імплантація у зону травми ацелюлярного фібринового матриксу людини (fibrin, Fb, n = 6). Оцінка рухової активності задньої паретичної кінцівки – шкала ВВВ, оцінка спастичності – шкала Ashworth; патогістологічне дослідження – забарвлення поздовжніх зрізів спинного мозку, отриманих у віддаленому періоді травми, методом імпрегнації сріблом. Фібриновий матрикс сприяє життєдіяльності, росту і диференціюванню інкорпорованих клітин неонатального головного мозку щура. Починаючи з 2–3-го тижня після імплантації у епіцентр травми рівень рухової функції паретичної кінцівки у групах Fb і NBC відповідав ~11-м балам ВВВ, у групі Tr – ~6-м балам ВВВ. Значущих відмінностей рівня рухової функції паретичної кінцівки між групами NBC і Fb, а також між групами Fb і Tr впродовж усього експерименту не зафіксовано. Достовірні відмінності показника між групами NBC і Tr виявлено через 2, 4 і 8 тижнів, а також через 3 і 5 місяців після травми. Істотну перевагу рівня спастичності у групі Tr над групами NBC і Fb виявляли, відповідно, через 6 і 7 тижнів після травми. Негайна імплантація фібринового матриксу у комплексі з алогенними клітинами неонатального головного мозку та без них спричиняє достовірний позитивний вплив на відновлення рухової функції після лацераційної ТрСМ.

Ключові слова: нейротрансплантація, фібриновий матрикс, травма спинного мозку, клітини неонатального мозку, відновлення локомоторної функції, спастичність

Цитологія і генетика
2022, том 56, № 2, 21-37

Current Issue
Cytology and Genetics
2022, том 56, № 2, 125–138,
doi: 10.3103/S0095452722020086

Повний текст та додаткові матеріали

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